Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,410	C→T	intergenic (+8/+2)	bhsB → / ← ldtC	small RNA BhsB/L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,410	0	C	T	100.0%	46.5 / NA	16	intergenic (+8/+2)	bhsB/ldtC	small RNA BhsB/L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

ATCAGTGCTAACGCGGGGACAAATCTGGGATCGCTGGAAGAGCAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATC  >  NC_000913/1169187‑1169643                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          aTCAGTGCTAACGCGGGGACAAATCTGGGATCGCTGGAAGAGCAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTtgat                                                                                                                                                                                                                >  2:207481/1‑251 (MQ=255)                           gggATCGCTGGAAGAGCAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATccc                                                                                                                                                                                       <  1:298768/250‑1 (MQ=255)                                                              aTGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                                                                                                                                   <  2:376232/251‑1 (MQ=255)                                                                gagaTGGGCGCAAAATCTTTCCGTATTACTTCTGTCAACGGTCCGAATACACTCCATGGAACAGCCGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGATGATTTTTTCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTATACGGGTTTTACAGCGTTTGTGGGCTCACTTGATGCCGGCGGGCATCCACCGGAATCCCGGAACGGACATCCATAACCTGTTGCATCATTTCAgagt                                                                                                                                                 >  2:334645/1‑248 (MQ=255)                                                                   aTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATTCCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCAACGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAg                                                                                                                                              <  1:397891/251‑1 (MQ=255)                                                                       gcgcAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtg                                                                                                                                          >  2:81085/1‑251 (MQ=255)                                                                                    ccGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCttt                                                                                                                              >  1:418613/1‑250 (MQ=255)                                                                                              tCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattg                                                                                                                     <  1:101679/249‑1 (MQ=255)                                                                                                                         ggAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGgcagc                                                                                         <  1:81085/250‑1 (MQ=255)                                                                                                                                                          cTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCg                                                       >  2:518741/1‑251 (MQ=255)                                                                                                                                                                  tGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGCCATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAACTGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCCATCTTCTCTGACAGCGGCTGCTGt                                               >  2:243136/1‑251 (MQ=255)                                                                                                                                                                             gctgATGTTTTCCCCGCGACATGCCGTGTCGCGGGTATTTTTTTAGCCGGGTGTTACAGCGGTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTGAAATGATTGCATTGCGCGATTCAGCGTAATTGGCAGCAGCTGCGGATAGTCATCAATCTTCGCTGACAGCGGCTGATGTACTTCAACCa                                     <  2:339675/250‑1 (MQ=255)                                                                                                                                                                                              gACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGAt                                                                               >  1:68225/1‑191 (MQ=255)                                                                                                                                                                                              gACATGCCGTGTCGCGGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACGTCAGCGTCAGTTTGTGCTGCATCTTGAAATGATGGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGAt                                                                               <  2:68225/191‑1 (MQ=255)                                                                                                                                                                                                           gcgGGGATTTTTTTATCCGGGTTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGATTGGTTCGGCAGAGACttt      >  1:130536/1‑251 (MQ=255)                                                                                                                                                                                                               ggATTTTTTTATCCGGGTTTTACAGCGTTTGTGTGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAAGTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATc  <  1:334645/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          ATCAGTGCTAACGCGGGGACAAATCTGGGATCGCTGGAAGAGCAGCTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCATTGCGCTATTCAGCGTAATTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATC  >  NC_000913/1169187‑1169643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑