Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,126,625	G→A	G157S (GGT→AGT)	yceH →	DUF480 domain‑containing protein YceH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,126,625	0	G	A	100.0%	166.0 / NA	52	G157S (GGT→AGT)	yceH	DUF480 domain‑containing protein YceH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/30);  total (22/30)

CACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACC  >  NC_000913/1126390‑1126867                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   cACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAAcc                                                                                                                                                                                                                                     <  1:447790/251‑1 (MQ=255)      aaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTAc                                                                                                                                                                                                                                <  1:9551/251‑1 (MQ=255)                 cGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCtttt                                                                                                                                                                                                                     >  1:379055/1‑251 (MQ=255)                        gTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAgtggt                                                                                                                                                                                                              <  1:454423/251‑1 (MQ=255)                              cAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTGTTCAGTGGTGAGGtt                                                                                                                                                                                                        <  1:377432/251‑1 (MQ=255)                                 aaTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGAGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGCGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCCGTGGTGAGGTTGa                                                                                                                                                                                                      >  1:46518/1‑250 (MQ=255)                                  aTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAg                                                                                                                                                                                                    <  1:218844/251‑1 (MQ=255)                                   tttGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGa                                                                                                                                                                                                      >  1:421956/1‑248 (MQ=255)                                     tGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGa                                                                                                                                                                                                      >  1:24042/1‑246 (MQ=255)                                     gggCGATCTGAAACTGTGCGCAGCGGAAGTGGCGTGAATCACCTCGTTGTTATTGCGTGGTTCCCAGACGCCGTGTGAACGTCTCTGCCGCGCCGCGCGAATGTATGAATTCAGCGATAGGGCGGAAGTGGAGGCTGCGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTTTGGTGTGTCTGGCTCGCGAACCGAGTAAACGCGGAAAACGCTACTTGGATCTTTTCAGTGGGGAGGTTGAAGATc                                                                                                                                                                                                 <  2:488191/250‑1 (MQ=255)                                             tGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGAGGAGTCGACGCTGGAACAAATGGCAAATCGCGAAGATGGGCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTTAGTGGTGCGGTTGAAGATCAGCcggcg                                                                                                                                                                                         >  1:60431/1‑251 (MQ=255)                                                     aGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACgg                                                                                                                                                                                  >  1:23270/1‑250 (MQ=255)                                                        gcAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGACGGatat                                                                                                                                                                              >  1:412247/1‑251 (MQ=255)                                                        gcAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGACGCTGGAACAAATGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAAACGAGTAAACGCGAAAACCGCGCAATGCATCTTTTCAGTGGTGAGGTTGAAGAACAGCCGGCGGTGACGGatat                                                                                                                                                                              >  2:293484/1‑251 (MQ=255)                                                          aGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGCACCAAGACCACAGGCGAACTGCGCAGCAGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGGAGGATATGt                                                                                                                                                                            >  1:22061/1‑251 (MQ=255)                                                                   ggCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCCGTGGTGAGGGTGAAGATCAGCCGGCGGTGGCGGATTTGTCGacagggg                                                                                                                                                                   >  2:12478/1‑245 (MQ=255)                                                                     cGTTAATCACCACGTTGTTATTGCGTTGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGGCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACTGATATGTCGAACGCGGtt                                                                                                                                                                 <  2:374042/251‑1 (MQ=255)                                                                         aaTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGACTTCAGCGATATGGCGGAAGTGGAGTCGCCGCTGGAACCAATGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTTGCCCGCGAACCGAGTAAACACGAAAACCGCCACATGCATCTTTTCCGTGGGGAGGTTGAAGATCAGCCCGCGGTGACGGAAAAGTCGGACGCGGTTggcg                                                                                                                                                             >  2:475189/1‑248 (MQ=255)                                                                                 cGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGACTGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                                      >  1:209544/1‑250 (MQ=255)                                                                                 cGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCAACTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGGCGGATATGTCGAACGCGGTTGACGGGGAtttt                                                                                                                                                     >  1:248489/1‑250 (MQ=255)                                                                                      ttATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGa                                                                                                                                                                                                   <  1:102402/200‑1 (MQ=255)                                                                                      ttATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCAGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGGCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGa                                                                                                                                                                                                   >  2:102402/1‑200 (MQ=255)                                                                                           gCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCcgcg                                                                                                                                           <  1:164532/251‑1 (MQ=255)                                                                                            cGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGt                                                                                                                                          >  1:203206/1‑251 (MQ=255)                                                                                               ggtgCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCTATATGGCGGAAGTGGAGTCGACGCTGGAACAACGGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGGCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCGACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGACGGATATGTCGATCGCGGTTGACGGTGATTTACAGGCCCGCGTCg                                                                                                                                        <  1:320635/250‑1 (MQ=255)                                                                                                  gCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAg                                                                                                                                     <  1:209148/250‑1 (MQ=255)                                                                                                   cccAGACGCCAGGTGAACTGGGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTTCATTCATCTTTTCAGTGGTGAGGTTTAAGATCAGCCGTCGGTGACTGATATGTCGAACGCGGTTGACGGTGATTGACAGGCCTGCGTCGAAGcc                                                                                                                                   <  2:60431/251‑1 (MQ=255)                                                                                                   cccAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGAGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGGGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCGCGCGTCGAAGcc                                                                                                                                   <  2:236169/251‑1 (MQ=255)                                                                                                        aCGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGa                                                                                                                              <  1:293484/251‑1 (MQ=255)                                                                                                         cGCCAGGTGAACGGCGCCGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATTGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGaa                                                                                                                             <  2:209544/251‑1 (MQ=255)                                                                                                                   aCTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAGCTGGCAAATCGCGAAGATGGTCCTTTTGGGGTGCGTCTGGCCCGCTAACCGATTAAACGCGAAAACCGCTACATTCATCTTTTCAGTGGCGAGGTGGAATATCAGCCGGCGGTGACGGATATGTCGAACGCGTTTGACGGTGATTTACGGGCCCGCGTCGAAGCCCTGGAAATCGAAGTgg                                                                                                                   <  1:310464/251‑1 (MQ=255)                                                                                                                    cTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGGAATCGAAGTGGc                                                                                                                  >  1:406239/1‑251 (MQ=255)                                                                                                                               cgccgcgcGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTTGCAAATCGCGAAGATGGTCCTTTAGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGGTGACGGTGATTTACAGGCCTGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAAc                                                                                                       <  1:62030/251‑1 (MQ=255)                                                                                                                                      cgAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGGGGCAGAACTGCAACAGCGTCt                                                                                                >  1:194014/1‑251 (MQ=255)                                                                                                                                        aaTGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAAACGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGAGCAGCCGGCGGTGACGGATATGTCGAAAGCGGTTGACGGTGATTTACAGGCCCGCGTCGGAGCCCTGGAAATCGAAGTGGTAGAACTGAAACAGCGACTTg                                                                                              >  1:134337/1‑251 (MQ=255)                                                                                                                                             aTGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTc                                                                                          <  1:55659/250‑1 (MQ=255)                                                                                                                                             aTGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTc                                                                                          <  1:214944/250‑1 (MQ=255)                                                                                                                                             aTGAATTCAGAGATATGGCGGAAGTGGAGTCGTCGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGGGTCTGGCCCGCGAACCGAGTAAACGGGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGGCGAAGCCCTGGAAATCGAAGTGTCAGAACTGAAACAGCTTCGTGATTc                                                                                          <  2:367457/250‑1 (MQ=255)                                                                                                                                                aaTTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCACATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAAACGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGcc                                                                                                                                                                                             >  2:308405/1‑148 (MQ=255)                                                                                                                                                aaTTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGcc                                                                                                                                                                                             <  1:308405/148‑1 (MQ=255)                                                                                                                                                       cGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGccc                                                                               <  2:23270/251‑1 (MQ=255)                                                                                                                                                        gATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCGTGTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCa                                                                              <  2:134337/251‑1 (MQ=255)                                                                                                                                                                ggAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGGAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGgag                                                                      <  2:421956/251‑1 (MQ=255)                                                                                                                                                                   aGTGGAGTCGACGCGGGAACAACTGGCAAAGCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGAtt                                                                   <  2:406239/251‑1 (MQ=255)                                                                                                                                                                       gAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGTGAGATTAAAg                                                               <  2:145454/251‑1 (MQ=255)                                                                                                                                                                             aCGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGCCGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTCGATTCGTTGCTGGCCCATCCGGGAGATTAACGTGaaa                                                          >  2:124607/1‑250 (MQ=255)                                                                                                                                                                                           ggCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCg                                             <  1:12478/249‑1 (MQ=255)                                                                                                                                                                                                     gAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACGGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAAAGCGGTTGACGGTGATTTCCAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCTGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGGGATTAAAGAGAAAAAATTACGTATCGGCGAAGTGGGAt                                 >  1:253774/1‑251 (MQ=255)                                                                                                                                                                                                           ggTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCGTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTTGGATTAGGTg                           <  2:361130/251‑1 (MQ=255)                                                                                                                                                                                                                  ttgtgaagCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACAGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGGTGACGGTGATTTACAGGCCCGCGTCGAAGACCTGGAAATCGAAGCGGCAGAACTGAAACAGCGTCTGGATTCGTTGCTGGCCCATCTGGGAGATTAAAGGGAACAAATTACGTATCGGCGGAGTGGGGTTAGGTGGGATTgt                    >  2:112583/8‑250 (MQ=255)                                                                                                                                                                                                                  ttGTGGTGCGTCTGGCCCGCGAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTg                     <  2:63068/250‑1 (MQ=255)                                                                                                                                                                                                                                    gcgAACCGAGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTAcc  <  1:133677/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   CACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACC  >  NC_000913/1126390‑1126867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑