Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,702	C→T	Q302Q (CAG→CAA)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,702	0	C	T	95.7%	144.3 / ‑0.9	48	Q302Q (CAG→CAA)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (28/17);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

CTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATA  >  NC_000913/1121475‑1121947                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      cTTCAATTAGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGAGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGAAACCCATAGAATTGCGGGCCGTTTacagaacaga                                                                                                                                                                                                                                 >  2:183495/1‑250 (MQ=255)        tCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTc                                                                                                                                                                                                                          <  1:506346/250‑1 (MQ=255)         cGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAAGTGCGGGCCGTTTACAGAACAGAATGCTTCa                                                                                                                                                                                                                         <  2:129142/250‑1 (MQ=255)           cATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAg                                                                                                                                                                                                                      <  1:491663/251‑1 (MQ=255)            ataataGCTCCCTGTTGAATATACTGGTTATTTATACATGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTGAACTGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCCGTCTGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGt                                                                                                                                                                                                                     <  2:415062/251‑1 (MQ=255)             taataGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTCCCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGtg                                                                                                                                                                                                                    >  1:517559/1‑251 (MQ=255)                        tGTTGAATAAACTGGCTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTAAGCTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCACTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTCCCAGTTCGTTGACTGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAAAATGCTTCAAAGTGCTGCATAGcat                                                                                                                                                                                                         >  2:451329/1‑251 (MQ=255)                           tGAATACACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAACGTGCTGCAAAGcattc                                                                                                                                                                                                       >  1:139177/1‑250 (MQ=255)                            gAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGACGCTACGATTTTTATTGTTTAACGGACCAGCGTCCCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGCTGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGcattcat                                                                                                                                                                                                     >  1:92081/1‑251 (MQ=255)                                    tGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCa                                                                                                                                                                                              >  1:411910/1‑250 (MQ=255)                                      gTTATTTATACTGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAGTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAg                                                                                                                                                                                           <  2:451917/251‑1 (MQ=255)                                             ataCAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCTATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGt                                                                                                                                                                                      >  1:233688/1‑249 (MQ=255)                                                   gTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAAc                                                                                                                                                                              <  2:66903/251‑1 (MQ=255)                                                     aaaaTAACCTAATGACAACAGGAAGCTACGATTTTCATTGTTTAACGGACCAGCGTAACGTTTCCCCGGCGAGGAATGCCACCAGCGAGTCATCAGTCAGTGCGATGCTTTCACCAACCTGTTGCTCTTCACGTACCAGTTCGATGACTGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAACGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTg                                                                                                                                                                            >  1:338826/1‑251 (MQ=255)                                                              tAATGACAACAGGAAGCTCCGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATAGCACAAGCGTGTAATCAGTCAGTGAGTTGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGACGTTTCCAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCATcgcg                                                                                                                                                                   >  2:79883/1‑251 (MQ=255)                                                               aaTGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGAACCAGCGAGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCCTTTCTTCAAAGACGGTAGCGTACCTGCCCAGCGCgg                                                                                                                                                                  >  2:235378/1‑251 (MQ=255)                                                                      acaGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGGGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGTCCAGCGCGGTTGGGGc                                                                                                                                                           <  2:109536/251‑1 (MQ=255)                                                                           aaGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTg                                                                                                                                                       >  2:454750/1‑250 (MQ=255)                                                                                 cGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGATTTCAGCAACCTGTTGCTCTTCACGTAACAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGATTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAagcagc                                                                                                                                                >  1:483256/1‑251 (MQ=255)                                                                                  gATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACTCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTCACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGcc                                                                                                                                               <  1:193257/251‑1 (MQ=255)                                                                                  gATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGcc                                                                                                                                               >  1:203483/1‑251 (MQ=255)                                                                                      tttATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCcgcg                                                                                                                                           >  2:110048/1‑251 (MQ=255)                                                                                       ttATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCgcgc                                                                                                                                          >  1:118979/1‑251 (MQ=255)                                                                                              ttAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGc                                                                                                                                    >  1:412178/1‑250 (MQ=255)                                                                                                                gTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATg                                                                                                                 >  1:448703/1‑251 (MQ=255)                                                                                                                   tCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACg                                                                                                              >  1:386045/1‑251 (MQ=255)                                                                                                                           gAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTg                                                                                                                                                                            >  1:517045/1‑181 (MQ=255)                                                                                                                           gAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATGGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTCCAAAGACGGTAGCGTAACTg                                                                                                                                                                            <  2:517045/181‑1 (MQ=255)                                                                                                                            aGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTgg                                                                                                     >  2:291777/1‑251 (MQ=255)                                                                                                                                 tGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGgcgc                                                                                                  >  1:79007/1‑249 (MQ=255)                                                                                                                                               cagcaggtagggCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGGCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAACGCTGCAAAGTGCTGCAAAGCAGTCATTTCTTCAAAGACTGTAGCTTAACTGCCCAGCGCGGTTGGGGCGTTGAGGCAGCCCTCGCAGCCGCAACTGGTCTCGGTGCGATGACGTGCAGTTGGCGCAGAAGCCGTACCGAg                                                                                   <  2:338826/239‑1 (MQ=255)                                                                                                                                                   agtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGaa                                                                                >  1:396979/1‑249 (MQ=255)                                                                                                                                                   agtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGaa                                                                                <  2:396979/249‑1 (MQ=255)                                                                                                                                                        gtGCGATGCTTTCAGCAAACTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACACATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGACGCAGCCCGCGCAGCCGCAACTGCGCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCg                                                                          >  2:209342/1‑250 (MQ=255)                                                                                                                                                             aTGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTaaa                                                                    <  2:79007/251‑1 (MQ=255)                                                                                                                                                                             gTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGATGAATACTCGATTAAAACCGCTGGCGACCAg                                                     <  1:317756/250‑1 (MQ=255)                                                                                                                                                                                  tctTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACg                                               >  1:244589/1‑251 (MQ=255)                                                                                                                                                                                     tCACGTACCAGTTCGATGAATGGGTCGTTGACCGGCAACCCATGGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCAGTCATTTCGTCAAAGACGGTAGCGTAACTGCCCAGCGCGGGTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGGGCAGGTGGCGCATAATCCGTACCGAGGAATACTCGATTAAAGCCGCTGGCGACCAGTTCACg                                               <  2:244589/248‑1 (MQ=255)                                                                                                                                                                                            ccAGTTCGATGAATGTGTCGTTGCCCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGt                                     <  2:411910/251‑1 (MQ=255)                                                                                                                                                                                             cAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGtt                                    >  2:415887/1‑251 (MQ=255)                                                                                                                                                                                                ttCGATGAATGTGTCGTTGACAGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTACAACCGCTGGCGACACGTTCACGCAATGCCTGTTGGt                                 >  2:270270/1‑251 (MQ=255)                                                                                                                                                                                                    aTGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAat                             >  1:316236/1‑251 (MQ=255)                                                                                                                                                                                                    aTGAATGTGTCGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAat                             >  1:9457/1‑251 (MQ=255)                                                                                                                                                                                                      gaatttggcGTTGACCGGCAACCTATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAata                            <  2:233688/242‑1 (MQ=255)                                                                                                                                                                                                              cGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGttt                    <  1:454750/250‑1 (MQ=255)                                                                                                                                                                                                              cGTTGACCGGCAACCCATAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTGTGCGATGACGTGCATGTGGCGCAGAATCAGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTg                   <  2:517559/251‑1 (MQ=255)                                                                                                                                                                                                                               tAGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAAt   >  2:369181/1‑250 (MQ=255)                                                                                                                                                                                                                                aGAATTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATa  <  1:291777/250‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATA  >  NC_000913/1121475‑1121947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑