Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,088	C→T	intergenic (‑133/+154)	bssS ← / ← dinI	regulator of biofilm formation/DNA damage‑inducible protein I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,088	0	C	T	100.0%	45.2 / NA	14	intergenic (‑133/+154)	bssS/dinI	regulator of biofilm formation/DNA damage‑inducible protein I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

AGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAAT  >  NC_000913/1120876‑1121331                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aGCGCATCATAGCTATCAACGGTGCTGATGTCCCCCCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATTGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACATATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGtcatt                                                                                                                                                                                                               <  2:393279/251‑1 (MQ=255) aGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGtcatt                                                                                                                                                                                                               <  1:454241/251‑1 (MQ=255)                         cTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGc                                                                                                                                                                                        >  1:6531/1‑250 (MQ=255)                                cccACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAattatt                                                                                                                                                                                 <  1:125776/250‑1 (MQ=255)                                     ccTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAgg                                                                                                                                                                           >  1:5943/1‑251 (MQ=255)                                                                                tcatGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGtaaaataaa                                                                                                                                 >  2:73863/1‑250 (MQ=255)                                                                                                                        aGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCAtattat                                                                                        <  1:176647/251‑1 (MQ=255)                                                                                                                                  cGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGGGTAGAAATTTTGTTCTCAAAATTCGTAAGTAAATTAATAAAGCCGGGGTGACCGGGTAAAATAGGTCTCAGCATATTATTCGCTGACaa                                                                              <  2:265038/251‑1 (MQ=255)                                                                                                                                            tGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACTGAATTTTTAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTGTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCAt                                                                     <  1:437249/250‑1 (MQ=255)                                                                                                                                                                                          ttAAAGGGGGAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGttt                       <  1:73863/250‑1 (MQ=255)                                                                                                                                                                                               gggggAAACAGATAAATTTCTGTTGTTAAGATTTATATGAACAATAAAACAGCATGTCAGTCATATTTTTTGAGCATATTGTGCAATTATTTTGATGAAGTGTAGAAATTTTGTACTCAAAATGCGTAAGTAAAATAAACAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATGTTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCGGGAGAATTTCACGAATGCGCTGTGTAtcttc                 <  2:210049/251‑1 (MQ=255)                                                                                                                                                                                                  ggAAACAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCttt              >  1:507521/1‑251 (MQ=255)                                                                                                                                                                                                    aaaCAGATAAATTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTATAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCCCTGAAAAACCAGTCATCGGCGCTTTCCCACGTTTTCTTGAGAATTTCAATAATGCGCTGTTTATCCTCttttt            >  2:106764/1‑250 (MQ=255)                                                                                                                                                                                                              aTTTCTGTTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAAt  >  1:135768/1‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTTTTTCCATCATGGCCCCCTAATTCGTGTTACTAGACAACGTTATTCGAGGTTCAATGCGTTTTTATCTGAAGCAACTTCAGTATACCAATAAGTAAGGGTATTCACCGAATTTTTAAAGGGGGAAACAGATAAATTTCTGCTTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAAT  >  NC_000913/1120876‑1121331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑