Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,620	T→A	V245V (GTT→GTA)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,620	0	T	A	100.0%	115.6 / NA	35	V245V (GTT→GTA)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/15);  total (20/15)

CGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAA  >  NC_000913/1098384‑1098864                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     cGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTTGTGTTCATGTTGTGGAAGATGTCCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGtt                                                                                                                                                                                                                                        <  1:191906/251‑1 (MQ=255) cGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGtt                                                                                                                                                                                                                                        <  2:323365/251‑1 (MQ=255)    aCCCGTAAAGCGTGGCCTGGCGTGCAAAGCGTCGCCGGACGGGAATAACTGTCTGCATTTCTGAGCCAATGTCGGGTATAGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCTCAATGTTGGa                                                                                                                                                                                                                                     <  2:514925/251‑1 (MQ=255)                           gCAAAGCTTTGCCGGACGGGAAGATCTGTCTGCAGTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGTAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCACCCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTa                                                                                                                                                                                                               <  2:363273/250‑1 (MQ=255)                                            gggAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGACAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGctc                                                                                                                                                                                             >  1:250505/1‑251 (MQ=255)                                             ggAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGctct                                                                                                                                                                                            <  1:26756/251‑1 (MQ=255)                                               aagaaCTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTgg                                                                                                                                                                                          >  1:366084/1‑251 (MQ=255)                                                      gTCTGCATTTCTTAGCCAAGGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATAAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATc                                                                                                                                                                                   <  2:375993/251‑1 (MQ=255)                                                      gTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCTGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATc                                                                                                                                                                                   <  2:39023/251‑1 (MQ=255)                                                                    gCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGAt                                                                                                                                                                      >  1:341808/1‑250 (MQ=255)                                                                                   tGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTcgcc                                                                                                                                                      >  2:123241/1‑251 (MQ=255)                                                                                   tGATTAATTTGTTACCGAATACCCCTGAAACCGCCGACATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTcgcc                                                                                                                                                      >  1:177423/1‑251 (MQ=255)                                                                                      ttAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCCCGCGTGACGATAACACCACCTGTCGCcgcg                                                                                                                                                   >  2:516306/1‑251 (MQ=255)                                                                                             gTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTAccc                                                                                                                                            <  2:441012/251‑1 (MQ=255)                                                                                               tACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTTCCCGt                                                                                                                                          >  1:504320/1‑251 (MQ=255)                                                                                                           cTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCt                                                                                                                              >  1:114105/1‑251 (MQ=255)                                                                                                                  cGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTCTCTCCTCAACCTAGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAACGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGACAGTCCGCTCTGGCAACATCCACGCGTGCCGATAACACCACATGTCGCAGCGATTACCCGTCCCGCTGACGCTGCGGAGt                                                                                                                       >  2:79817/1‑251 (MQ=255)                                                                                                                      ggtattattAATCAACAATTACTCGAAAAATTACCGGATGGCTCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAATTAAAAGGCGCAATGTGGGAGGTTTTTAATCGTGAACCCGTGCCGCCTGAATGTCCGCTTTGGTATCATCCACGCGGGACTATAACACCACATGTCGCCGCGATTGCCCGTGCGGCTGAAGCTGGTGAGTACAt                                                                                                                   <  2:506081/248‑1 (MQ=255)                                                                                                                         attattAATCAACAATTACTCGAAAAATTACCGGATGGCGCTTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGACGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAACAGGCGCAATGGTGGATGTTTTTAATCGTGAACCCTTCCCGCCTGAATGTCAGCTATGGCAACATCCACGCGTGACGCTAAAACCACATGTCGCCTCGATTACCCGTCCCGCTGCAGCTGTGGAGTCCAtttt                                                                                                                >  2:401100/1‑250 (MQ=255)                                                                                                                         attattAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTtc                                                                                                                >  1:125423/1‑251 (MQ=255)                                                                                                                                  caacaaTTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCa                                                                                                        <  2:495402/250‑1 (MQ=255)                                                                                                                                     caaTTAATCGAAAAATTACAGGATGGCGCGTATCTCCTCAACCTGTCGCGTGGTGTTCATGTTGAGGAAGAGGACCTGCTCGAGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGAAACATCCACGCGTGACGATAACCACACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGTAGTACATTTCTCGCACCATTGc                                                                                                    >  2:410174/1‑251 (MQ=255)                                                                                                                                              gAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGGGTTCATGTTGTGGCAGATGACATGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCACTGTTgg                                                                                                                                                                                                                                      >  2:521868/1‑112 (MQ=255)                                                                                                                                              gAAAAATTACCGGATGGCGCGTATCCCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGTCCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTgg                                                                                                                                                                                                                                      <  1:521868/112‑1 (MQ=255)                                                                                                                                                     tACCGGATGGCGCGTATCTCCTCAACAAAGCGCGTGGTGTTAATGTTGTGGAAGATGAACTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGAAATGTTGGCTGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTATGGCAACATCCACGCGTGACGACAACACCACATGTCGCCGCGATTACACGTCCCGCTGACGCTGTGGAGTACATTTATAGCACCATTGACCAGGTCGTAAAAg                                                                                      >  2:353360/1‑249 (MQ=255)                                                                                                                                                             ggCGCGTATCTCCTCAACCGGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCTAAAAAGGGGAGAGGGt                                                                            <  2:113486/251‑1 (MQ=255)                                                                                                                                                                   tATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCgg                                                                      >  2:160533/1‑251 (MQ=255)                                                                                                                                                                                           gtTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGct                                               >  1:424282/1‑250 (MQ=255)                                                                                                                                                                                                                   cTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCCATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGGCGATAACACCACATGTCGCCGCGATTAGCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGGAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGc                      >  1:393082/1‑251 (MQ=255)                                                                                                                                                                                                                       cgtcgcTGGATAGCGGCAAAGTAAAAGGCGCAATGTTTGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTTATAAAGCATCAGGATTCCTGCTATc                  <  1:353360/248‑1 (MQ=255)                                                                                                                                                                                                                          cgcTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCtt               >  1:407038/1‑251 (MQ=255)                                                                                                                                                                                                                               gATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGgaga                                                                                <  2:7311/181‑1 (MQ=255)                                                                                                                                                                                                                               gATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGgaga                                                                                >  1:7311/1‑181 (MQ=255)                                                                                                                                                                                                                               gATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTATAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCCCATGTCGCCGCGATTACCCGTCCAGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGCCCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCgg          >  2:455558/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACTCCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGaa  <  1:516306/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     CGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAA  >  NC_000913/1098384‑1098864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑