Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,412	C→T	P287S (CCG→TCG)	modC →	molybdate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,412	0	C	T	98.2%	167.5 / NA	54	P287S (CCG→TCG)	modC	molybdate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/26);  minor base G (0/1);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTGGCTATAAATGAACTGGGCAAT  >  NC_000913/797166‑797645                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ggTCAGGTGAAAACCTTGGGCGGGCAGGAGGAAGTGTGGGGCAGTAGCGGGATGAATCCGTGGCGGCCGAAAGAGAAACAAAGTAGCATTCTGAAAGTGACGGGGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCATTAAGCTGGCCGAACCGCTGAAAGATGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACcgtcgcc                                                                                                                                                                                                                                       >  2:238889/1‑250 (MQ=255)    cAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGCAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTCCGTATCCGCATTCAGGCTTCAGATGTTTCTCTGGTTTTACAGCCGTCgcagc                                                                                                                                                                                                                                     >  2:72766/1‑250 (MQ=255)    cAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCgcagca                                                                                                                                                                                                                                    <  2:106311/251‑1 (MQ=255)      ggTGAAAGCCTGTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCaaa                                                                                                                                                                                                                                  <  2:111692/251‑1 (MQ=255)              ccTTTGGCGCGCTGGCGGAAGTGTGGGGAAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCCACAAAGTCGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCAtt                                                                                                                                                                                                                          >  2:526826/1‑251 (MQ=255)                    gcgcgcTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAAc                                                                                                                                                                                                                    <  1:226/251‑1 (MQ=255)                     cgcgcTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACg                                                                                                                                                                                                                   >  1:378290/1‑251 (MQ=255)                        gcTGGTGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCAGAAAGAGCAACTAAGTAGCATTCTGAAAGTGACGGCGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTAt                                                                                                                                                                                                                >  1:453757/1‑251 (MQ=255)                        gcTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTAt                                                                                                                                                                                                                <  2:378688/251‑1 (MQ=255)                         cTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGCAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACACCCGTCGCAGCAAACCAGCATTCGTAACGAAtt                                                                                                                                                                                                               >  2:63764/1‑251 (MQ=255)                             aggaAGCGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCgg                                                                                                                                                                                                           >  1:437715/1‑251 (MQ=255)                                   tgtgGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGGAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCaaa                                                                                                                                                                                                      >  2:59582/1‑250 (MQ=255)                                      ggggCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAgt                                                                                                                                                                                                   <  2:343732/250‑1 (MQ=255)                                       gggCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAgttg                                                                                                                                                                                                 <  2:410963/251‑1 (MQ=255)                                          cAGTAGTGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCGAACCAGCATTCGTAACGTATTGCGGGCAAAAgttgtt                                                                                                                                                                                               >  1:152505/1‑250 (MQ=255)                                               gCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGt                                                                                                                                                                                         <  2:144773/251‑1 (MQ=255)                                                 gTGAAGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCATGAGCATCATCCGCATTACTCGATCACCGCGCTTGTGCTGGGCGCTCATCGTTATTGGGACAGTATGCTTGACGAACCGGTGCAAGCTGCGTTACGTAGCCGCAATCAGTGTTCCGATGATTCACTAGTTTTACATCCGTCGCAGCAAAGCAGCAGTCGAAACGTATTGCGGGCAAAAGTTGTTAATAGTTa                                                                                                                                                                                       <  2:1804/251‑1 (MQ=255)                                                   gatgaATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATg                                                                                                                                                                                     <  1:346359/251‑1 (MQ=255)                                                      gaATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGACCCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATgac                                                                                                                                                                                   <  1:249791/250‑1 (MQ=255)                                                          ccGTGGCTGCCGAAAGGGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACa                                                                                                                                                                               <  1:9574/250‑1 (MQ=255)                                                            gTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACAGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACg                                                                                                                                                                            >  2:450188/1‑251 (MQ=255)                                                                 tGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGAGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATCAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGcc                                                                                                                                                                         >  1:63469/1‑249 (MQ=255)                                                                  gCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCGTGAGCATCATCCGCAGTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAAGAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCGGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATGGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAg                                                                                                                                                                       <  2:208284/250‑1 (MQ=255)                                                                             aacaaAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtggaagtgga                                                                                                                                                            >  2:435783/1‑250 (MQ=255)                                                                               caaAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACTAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAACAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACt                                                                                                                                                         >  1:261723/1‑251 (MQ=255)                                                                                aaaGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTg                                                                                                                                                        >  2:160010/1‑251 (MQ=255)                                                                                                aGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGTGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGGCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAgttgtt                                                                                                                                                                                               <  1:511753/196‑1 (MQ=255)                                                                                                         gCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGCTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTggg                                                                                                                               >  1:10475/1‑251 (MQ=255)                                                                                                                  tcatcCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATc                                                                                                                       >  1:412021/1‑250 (MQ=255)                                                                                                                      ccGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGcc                                                                                                                   <  1:72766/250‑1 (MQ=255)                                                                                                                        gCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTATGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGt                                                                                                                >  1:195476/1‑251 (MQ=255)                                                                                                                            tACGCGTTGACCGCGCTGGCGCTGTGCGATCAGCATTTGTGTGTCAATAAGCTGGACGAACCGCGTCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTGTCTCTGGGTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGGATTGCGGTCAAGAGTTGTTAATAGTTATGACTACAACGGCCAGGGGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCGGTGGGCGCGTATCAGCCCTTggg                                                                                                             <  2:453757/250‑1 (MQ=255)                                                                                                                            tACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtggaagt                                                                                                                                                               >  2:146294/1‑200 (MQ=255)                                                                                                                            tACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgaggaagt                                                                                                                                                               <  1:146294/200‑1 (MQ=255)                                                                                                                                                gctggGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATc                                                                                         >  2:388871/1‑250 (MQ=255)                                                                                                                                                     gCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCt                                                                                   >  1:68245/1‑251 (MQ=255)                                                                                                                                                         tCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGGATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGcc                                                                               >  2:273274/1‑251 (MQ=255)                                                                                                                                                           aGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCtg                                                                             >  1:394512/1‑251 (MQ=255)                                                                                                                                                             cATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCtgtg                                                                           >  1:112870/1‑251 (MQ=255)                                                                                                                                                                ttGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGc                                                                         <  1:288556/250‑1 (MQ=255)                                                                                                                                                                    gtggcAATAAGCGGGACGGACCGCTGCAAGCTGCGCTACGGATCCGCATTCAGGATTCAGATGTTTCTCTGGGTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTGATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCAAGGGATGAACTGGCGATCAAACCTGGTCTGTGGCTGTAc                                                                    <  2:315727/247‑1 (MQ=255)                                                                                                                                                                    gggTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTAc                                                                    <  1:526826/251‑1 (MQ=255)                                                                                                                                                                     ggTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTAcg                                                                   <  1:450188/251‑1 (MQ=255)                                                                                                                                                                      gTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTCCGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTAcgc                                                                  <  2:3027/251‑1 (MQ=255)                                                                                                                                                                         aaTAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACgcgc                                                                >  1:396910/1‑250 (MQ=255)                                                                                                                                                                             aGCTGGACGAACCGCTGCAAGCTGCGCTACGCATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTTCAACCGTCGCAGCAAACCAGCATTCGTACCGTCTTGGGGGCAAACGTTGTTACTAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATg                                                                                                    >  1:530620/1‑210 (MQ=255)                                                                                                                                                                                        ccGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTc                                                <  2:437715/251‑1 (MQ=255)                                                                                                                                                                                             gatatgttctctacgttgccgagatCAGGCTTCCGATGTTGTGATGTTTTTTCAACCGTCGCAGCAAACCAGAAGGCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGGCGGCGGTAAAACGCTGTGGGCTCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGGATGCGCAAATTAAAAGTGTGTCGAt                                             <  2:288916/225‑1 (MQ=255)                                                                                                                                                                                                    ccgcTACGTATCCGAATTCAGGCTTCCGATGCTTCTCTGGTTTTACAACCGTCGCAGCAACCCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTg                                    <  2:10475/250‑1 (MQ=255)                                                                                                                                                                                                       cTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGCGTGTCGATAACCGCCTGAtt                                 >  2:405635/1‑251 (MQ=255)                                                                                                                                                                                                            tATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGAAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCAAGGTGGCAGTGGAAATGGAAGTCGGCGGCAAAACGCTGTGGGCGCGAATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGGGTCGATAACCGCCCGATTAAATc                            >  1:434950/1‑251 (MQ=255)                                                                                                                                                                                                                  cATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTCCGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTgg                      >  2:148603/1‑251 (MQ=255)                                                                                                                                                                                                                         gCTTCCGATGTTTCTCTGGTTTTACAACCGTCGCAGCAAACCAGCATTCGTAACGCATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTGGCTATaaa               <  2:26541/251‑1 (MQ=255)                                                                                                                                                                                                                                        ctGGTTTTACATCCTTCGCAGCAAACCAGCATTCGTAACGTATGGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTTTGTCGATAACCGCCTGATTAAATCAGGTGGCTATAAATGAACTGGGCAAt  <  1:23378/249‑1 (MQ=255)                                                                                                                                                                                                                                        ctGGTTTTACAACCGTCGCAGCGAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGTGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTGGCTATAAATGAACTGGGCAAt  <  2:152505/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          GGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGATGACCGCGCTGGCGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTACGCGCAAATTAAAAGTGTGTCGATAACCGCCTGATTAAATCAGGTGGCTATAAATGAACTGGGCAAT  >  NC_000913/797166‑797645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑