Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,409	T→G	intergenic (‑758/+28)	sgcX ← / ← yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,409	0	T	G	100.0%	247.5 / NA	70	intergenic (‑758/+28)	sgcX/yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/32);  total (38/32)

AAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCG  >  NC_000913/4532168‑4532653                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aaaCCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGa                                                                                                                                                                                                                                              <  1:184472/251‑1 (MQ=255)                   tcCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCtg                                                                                                                                                                                                                               <  1:30933/248‑1 (MQ=255)                   ccccAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGAGCACTGGCACTGTGTGGATCTGGGGCCGACATCGGCATTCCGCAAGTCTGACAGAAATGGTTATGTTGGGACATATGATGCTCCTTATGGTGACATATTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGTACCCtgttg                                                                                                                                                                                                                            <  2:254482/250‑1 (MQ=255)                       aGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGACTATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTAc                                                                                                                                                                                                                        >  1:263266/1‑251 (MQ=255)                          gTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCgg                                                                                                                                                                                                                     <  1:75859/251‑1 (MQ=255)                           tttGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCggg                                                                                                                                                                                                                    >  1:303485/1‑251 (MQ=255)                                    gCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGt                                                                                                                                                                                                            <  1:10392/250‑1 (MQ=255)                                      cGTTGCTGTCGCTGCATTACGCACAATATTGATCGCTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCGTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAATCAAAGGTAAAGCCTCGATATTGCCAGCGAGAAATACCGGGAACCTGTTGTTACCGGGCGATGGGTGCg                                                                                                                                                                                                         <  1:186464/251‑1 (MQ=255)                                         tGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAac                                                                                                                                                                                                      >  2:379038/1‑251 (MQ=255)                                             gTCGCTGCATTACGCACAATAGTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACAGCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAAGACCGGGAACCTGTTGGTACCGGGCGATGAGTGCgcactcg                                                                                                                                                                                                   <  2:119818/250‑7 (MQ=255)                                              tCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGcc                                                                                                                                                                                                 >  2:349314/1‑251 (MQ=255)                                                   gCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑Gc                                                                                                                                                                                           >  1:296448/1‑251 (MQ=255)                                                               atatTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTACTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGAAAAGCCTCGATATTGCAAGCCAGAAATACCGGGAACCTGTGGTAACCGGGCGAGCAGGGGGAACA‑GCCCCAAGCAAAAGCATTccc                                                                                                                                                                               >  2:176581/1‑251 (MQ=255)                                                                 atTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGTCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCcg                                                                                                                                                                              <  1:100908/250‑1 (MQ=255)                                                                          tGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTc                                                                                                                                                                    >  2:428660/1‑251 (MQ=255)                                                                                 gtgtgCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGc                                                                                                                                                             <  1:379038/251‑1 (MQ=255)                                                                                    tgCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGtt                                                                                                                                                          >  1:162860/1‑251 (MQ=255)                                                                                    tgCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGtt                                                                                                                                                          >  1:226991/1‑251 (MQ=255)                                                                                     gCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGttt                                                                                                                                                         >  2:446870/1‑251 (MQ=255)                                                                                           ggTGCCGACATCGGCAGTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGGGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCGCGTAGCGTTTCg                                                                                                                                                       <  2:44845/247‑1 (MQ=255)                                                                                              gCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGAt                                                                                                                                                >  1:477760/1‑251 (MQ=255)                                                                                              gCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGAt                                                                                                                                                >  1:157649/1‑251 (MQ=255)                                                                                                       ggCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTc                                                                                                                                       >  2:516250/1‑251 (MQ=255)                                                                                                         cATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCgg                                                                                                                                     >  1:159484/1‑251 (MQ=255)                                                                                                             ccGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACGCGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGGCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCt                                                                                                                                 <  1:303794/251‑1 (MQ=255)                                                                                                                 aaGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACt                                                                                                                             >  1:89738/1‑251 (MQ=255)                                                                                                                   gCCTGACAGAACGGTTCATGGTGTGACATATGATGCTCCTTATTGTGACATCTTGATTAATAAACATACTGAATAGGTATTGGGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATGCCCTCGCGTATGTCACTTAGCGTTTCGGCGCGAGTTTTAGTTCGGCCCTGACTTc                                                                                                                           <  2:296448/251‑1 (MQ=255)                                                                                                                    ccTGACAGAACTGTTCATGTTATGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTcg                                                                                                                          <  2:337155/251‑1 (MQ=255)                                                                                                                     cTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGGCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTcgg                                                                                                                         >  1:539049/1‑251 (MQ=255)                                                                                                                       gACAGAACTTTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATGCCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGTCCCA‑GCCAAAGCAGTCCCGCTCGTTTGTCACGTAGCGTTGCGGCGTGATGTGTAGTTCGGCCCGGGCTTcggc                                                                                                                        <  2:524737/250‑1 (MQ=255)                                                                                                                                  tCATGTTGTGACATATGATTCTCCGTATGGTGACATCTTGATGAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCTCGTAGGTCACGTAGCGTTTCGGCGCGAGGTGTAGTTCGGCCCTGACTTCGGCGGCGAAGttat                                                                                                             <  2:306766/250‑4 (MQ=255)                                                                                                                                  tCATGTTGTGACATATGATGCTCCTTCTGGTGACCTCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGCTGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATc                                                                                                            >  2:484551/1‑251 (MQ=255)                                                                                                                                   tgttttgtGAAATTTGATGCTTCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATAAGATCAAAGCAAATGTAAAGCCTCGATAGTGCCAGCCATAAATACCGGCAACCTTTTGTTCACGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGGCTTCGGCGGCGATGTCATc                                                                                                            <  2:278260/246‑1 (MQ=255)                                                                                                                                   cATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATc                                                                                                            <  1:235905/250‑1 (MQ=255)                                                                                                                                         gtgACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAgg                                                                                                      >  2:455724/1‑250 (MQ=255)                                                                                                                                                  gatgcgtcTTATGGGTACATCTTGATTAATAAACATACTGAATATGTATTGGGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCATCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTcc                                                                                            <  2:413341/244‑1 (MQ=255)                                                                                                                                                     gCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagc                                                                                         >  2:467133/1‑251 (MQ=255)                                                                                                                                                                 gaaaTCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCAt                                                                              <  2:303485/247‑1 (MQ=255)                                                                                                                                                                 gACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATg                                                                             >  1:94063/1‑251 (MQ=255)                                                                                                                                                                    aTCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGt                                                                                                                                                                     >  1:512310/1‑160 (MQ=255)                                                                                                                                                                    aTCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGt                                                                                                                                                                     <  2:512310/160‑1 (MQ=255)                                                                                                                                                                       ttGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGc                                                                       >  1:163657/1‑251 (MQ=255)                                                                                                                                                                        tgagtaataaACATACTGAATATGTATTGTGCAGATGATACAGCAGATCAAAGCAAAGGTAAGGCCTCGATATTGCCAGCCAGAAATACCGTGAACCTGTTGTTACCGGGCGATGAGTGCGATCACGCCCCA‑GCTAAAGCATGCCCTCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCTGCCCTGATTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGc                                                                       <  1:44333/246‑1 (MQ=255)                                                                                                                                                                        tGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCTGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGc                                                                       <  1:467133/250‑1 (MQ=255)                                                                                                                                                                        tGATTAAAAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGTGAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCTCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTGCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGc                                                                       <  2:89738/250‑1 (MQ=255)                                                                                                                                                                            taataaACATACTGAATATGTATTGTGCAGGTGATACATCAGTTCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGCCCGGATTCGCTTCCAGCCAGCGGCGCATGGGCAGCCAttt                                                                  >  2:212070/1‑251 (MQ=255)                                                                                                                                                                             aataaACATACAGAATAGGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGAGGTTTAGTTCGGCCCTGAGGTCGGCGGCGAAGGCATCGACAGGAGTCTCTTCCAGGCATCGGCGCATGGTCAGCCAtttt                                                                 <  2:263266/251‑1 (MQ=255)                                                                                                                                                                                 aaCATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCgg                                                             >  1:174827/1‑251 (MQ=255)                                                                                                                                                                                   cATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTTAGCCATTTTGCGGCt                                                           <  1:428660/251‑1 (MQ=255)                                                                                                                                                                                   cATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCt                                                           >  1:371488/1‑251 (MQ=255)                                                                                                                                                                                   cATACTGAATATGTATGGTGCAGGTGAGACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATTGTCAGGATTCGCTTCCAGCCAGCGGCTCATGGTCAGCCATTTTGCGGCt                                                           <  2:371488/251‑1 (MQ=255)                                                                                                                                                                                         gAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGCCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCCTTTTGCGGCTTCATAc                                                     >  2:95519/1‑251 (MQ=255)                                                                                                                                                                                                 attgtgcagtggaaccATCAGATCAAAGCAAAGGTAAAGCCGCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGATCACGCCCCA‑GCCAAATCATGCCCGCGCGTAGGTCACGGAGCGTTTCGGCGCGATGTTTAGGTCGGCCCTTACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGTCATGTGCGCATGGTCAGCCATTTTGCGTCTTCATACTGGTccc                                              <  2:54378/235‑1 (MQ=255)                                                                                                                                                                                                 aTTGTGCAGGTGATACAACAGATCAAAGCAAAGGTAAAGCCTTGATATTGCCAGCCAGAAATACCGGGAACCTGGTGTTACCGGGCGATGAGTGCGGACACGCCCCA‑GCCAAAGCCTTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATTTTTAGTACGGCCCTGACTTCGGTGGCGATGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCACTTTGCGGCTTCATTCTGGTcca                                              <  2:2879/250‑2 (MQ=255)                                                                                                                                                                                                    gtgCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGcc                                          >  1:89839/1‑251 (MQ=255)                                                                                                                                                                                                    gtgCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGc                                           <  1:516250/250‑1 (MQ=255)                                                                                                                                                                                                       aaGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTGCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTc                                       <  2:122858/250‑1 (MQ=255)                                                                                                                                                                                                        aGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTcc                                      >  1:289617/1‑251 (MQ=255)                                                                                                                                                                                                         ggTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATAGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCCGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt                                     >  1:307005/1‑251 (MQ=255)                                                                                                                                                                                                           tGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTgg                                   >  2:430692/1‑251 (MQ=255)                                                                                                                                                                                                             ttACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCCGGATTCGCTTCCAGCCAGCGGCGCCTGGCCAGGCATTTTGCGGCTTCCTACAGGTCCCAGCCTTCCTGGTc                                 >  2:108362/2‑251 (MQ=255)                                                                                                                                                                                                             aTACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTc                                 >  1:133939/1‑251 (MQ=255)                                                                                                                                                                                                                   cAGATCAAAGCAAAGGAAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCttcccg                           >  2:354625/1‑246 (MQ=255)                                                                                                                                                                                                                    aGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGCAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGt                          >  1:463617/1‑251 (MQ=255)                                                                                                                                                                                                                     gATCAAAGCAAAGGAAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAAAACGCCCCA‑GCCACAGCATTCCCGCGCGTAGGTCACGAAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGt                          >  1:343748/1‑250 (MQ=255)                                                                                                                                                                                                                      aTCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTAc                        >  1:429490/1‑251 (MQ=255)                                                                                                                                                                                                                        caaagcaaagGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCCGGATTCGCTTCCAGCCAGCGGAGCATGGTCAGCCATTTTGCGGCTTCATAACGGTCCCAGCCTTCCTGGTCTGCCCGTAcc                       >  2:6402/1‑250 (MQ=255)                                                                                                                                                                                                                               aagGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGATACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAACGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGCTGTTTAGTTCGGCACTGACTTCGGCGGCGAAGTCATCGTCCGGATTCGCTTCCAGCCAGCGGCGCATGGGCAGCCAATGTGCGGCGTCATACCGGTCCCCGCCTTccgtggcatacagtaccatttccgc               >  2:104256/1‑228 (MQ=255)                                                                                                                                                                                                                                    aaaGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACg           <  1:176581/250‑1 (MQ=255)                                                                                                                                                                                                                                            gATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCg  <  1:484551/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCA‑GCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCG  >  NC_000913/4532168‑4532653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑