| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,396,772 | T→C | F236F (TTT→TTC) | amiB → | N‑acetylmuramoyl‑L‑alanine amidase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,396,772 | 0 | T | C | 98.6% | 234.2 / ‑4.8 | 72 | F236F (TTT→TTC) | amiB | N‑acetylmuramoyl‑L‑alanine amidase B |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (37/34); total (38/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGA > NC_000913/4396532‑4397016 | ggCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCCGGACCCTGGCGCTATCGGCCCCGGGGGTACGCGGGCGAAAAATGTCACCATCGCCATCGCCCGCAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAACGGCGttt > 2:191939/1‑251 (MQ=255) gcgcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTTGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCGGGT‑GTTCAAAGGCGtttt < 2:198280/251‑1 (MQ=255) cgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGACAGGACCCTGGCGCTATCGGCCCCGGGGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAAc > 1:194302/1‑251 (MQ=255) gcAATCCGTTTCAAACGGAAAGAAACCGCACTACGGGTGTTATCAGCAGAAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTAGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAACAACGCCATCGCGCGGAAATTGCGTAATTTGCTCAAAGACCATACGAT‑GTTCAAAGGGGTTTTAAcc > 2:352714/1‑251 (MQ=255) aaTCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCCTCGCGCGGAAATTGCGTACTTTGCTCAATGACGCTCCGAT‑GTTCAAAGGCGTTTTaa > 2:403171/1‑247 (MQ=255) aaTCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTaa < 1:403171/247‑1 (MQ=255) aaaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGa < 1:37299/251‑1 (MQ=255) aaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGGCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGAtt < 1:336406/251‑1 (MQ=255) aCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACTCTGGCGATAAAAGTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCTGCCCCGGTGGTACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTATCCCGTGACGGGGATTa < 1:318279/251‑1 (MQ=255) aaGTAACCGCACTACGGGGGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCAGCGCTAGTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTa < 2:431747/251‑1 (MQ=255) gTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAAGGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACTGGGATTACTTTAtc < 1:434446/251‑1 (MQ=255) cGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑t < 2:27168/251‑1 (MQ=255) ttATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCCTCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAgg > 1:125833/1‑250 (MQ=255) aTCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTg < 1:266335/250‑1 (MQ=255) cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGAt < 1:181558/250‑1 (MQ=255) gcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAgggaagtg > 1:530782/1‑244 (MQ=255) gcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGAtgtg < 1:306324/251‑1 (MQ=255) gcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGAACCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGCAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTAATTTATCTCGG‑TGATGGGGCGCAGTGAtgtg > 2:90242/1‑251 (MQ=255) aaTACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAAGACCCTGGCGCTATCGGCCCCGGGGGTACGCGGGAGAAAAATGTCACCCTCGCCATCGCGCGTAAATTGCGTACTTTGCTTAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACg > 1:229352/1‑251 (MQ=255) aaCGGTAACGCGTCCGGCAGCGCGCGAGACGGCTAACACTGGCGATAAAATTATCCTCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAGTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTa < 2:263203/250‑1 (MQ=255) aCGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTaa < 1:219472/251‑1 (MQ=255) aaCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCaaa < 1:244282/251‑1 (MQ=255) gcgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAACTTATCATCGCTATTGATGCCGGACACGGCGGTCAAGACCCTCGCGCTATCGGCCCCGGGGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAAATGCGTCCTTTACTCAATGACAATCCGATAGTCCAAAG‑CGTTTTAACCCGGGAAGGGGATTACTTTATATCGGATG‑TGGGgcgcctggaggtataaccgaagcaaaaac > 2:376927/1‑226 (MQ=255) gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGGACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCTCGTAAGCAAAAC‑Gcc < 2:306214/251‑1 (MQ=255) cgtcAGCCCGCGCGACGGCTAACACTGGCGATAAAATTATCAGCGCGATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGTCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACGTTATCTCGG‑GGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCaaa < 2:316549/248‑2 (MQ=255) cgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAAccc < 1:472283/176‑1 (MQ=255) aaCACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCAc < 1:383990/249‑1 (MQ=255) cacTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTg > 1:201596/1‑251 (MQ=255) tGGCGATAAAATTATCATCGCTCTTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCCGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGGACTTTGCTCAATGACGCTCCGAT‑GTTCAAAGGCGTTTTAACCCGGGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCCATTTCCTCGTGTCGATTCACGCTggtg > 2:516394/1‑248 (MQ=255) ggCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGAACCGGTGGTACGCGGGAGCAAAATGTAACCAGCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGAAGATCCGCT‑GTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGG‑GGGTGGGGCGCCGTGATGTGGCACGTAAGCAAAAA‑CCCAAATTCCTCGGGTCGCTTCAAGCtgctgc > 2:350149/1‑251 (MQ=255) aTAAAATTATCATCGCTATTGATGCCGGACACGGCGGGAAGGACCCTAGCGCTATCGGCCCCGGAGAGACGCGGGAGGAAAATGTCACCAACGCCATCTCGCGTAAATTGCGTACTTCGCTCAATGACGATACGAT‑GTTTAAAGGCGTTTTAACCCGTGGAGGAGATTAATTTATCTCGG‑TGGTGGGGCGAAGGGATGTGGGACGGAAGCAAAAC‑GCCCATTTCCTCggggcagttcacgactggacctcc > 2:295329/1‑228 (MQ=255) cGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAATGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAAACGCAGt < 1:333802/250‑1 (MQ=255) tATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCAAGCTGATGCCGCACCGAACCGCAGTGCg > 1:328248/1‑250 (MQ=255) ttGATGACGGACACGGAGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGGACGCGGGGGCAAAATGTCACCATCGCCATAGCGCGTAAATTGCGTACTTTGCTCAATGACGAACCGAT‑GTTCAAAGGAGTTTTAAACCGTGGCGGGGAGTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTACG‑AAAACAGCCCATTTCCTCGGGTCGCATCACGCTGATTCCCCCACGAACCGCAGTGccgct > 2:480559/1‑247 (MQ=255) gCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGgcgc > 1:111218/1‑251 (MQ=255) ggACACGGCGGTCAGGACCCTGGCGCTATCGGCACCGGTGGGACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGACCCGCAGTGCGACTTGCGCTTc > 1:527083/1‑251 (MQ=255) acGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGGGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCCCGCTGATGCCGCCCCGACCCGCCGTGCGACTGGCGCTTCCGtt > 1:1781/1‑250 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTTATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTg < 1:299201/251‑1 (MQ=255) gTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTg > 2:298163/1‑251 (MQ=255) gACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGACAAAAACGACACCCTCGCCACCGCGCGTAAATTGCGTACTTTGCTCAATGAAGATCCGAA‑GTTCAAAGGCGTCTTACCCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGCGTCGATTAACGCTGATGCCGCACCGAACCGCGGTGCGACTGGCGCTTCCGTATGGGTActctc > 1:75586/1‑251 (MQ=255) cccTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTa < 2:422133/251‑1 (MQ=255) gcgcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCAGCGCCATCGCGCGTAAATTGCGTACTTTGATCAATGACGATCCGAG‑GTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGt < 2:415044/251‑1 (MQ=255) cGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGGAAATTGCGTACTTTGCTCAATTACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGGGCTCTCTAACCGTCGCGcc < 2:192573/250‑1 (MQ=255) cGGCCCCGGTGGTACGCGGGAGAAAAATGTAACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑CGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACAGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGcc > 1:487684/1‑250 (MQ=255) gCCCCGGCGGTACGCGGGAGACAAATGCCACCATCGCCATCGCGCGCAAATTGCGTACTTTGCTCAATGACAATCCGCT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTACCTCGG‑CGCTGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGACGCACCGAACCGCAGAGCGACTGGCGCTTCCGCATGCGTGCTCTCTAACCGTCGCGCCAAc > 2:68508/1‑251 (MQ=255) ccGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGt < 1:191939/251‑1 (MQ=255) cGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTg < 2:240865/251‑1 (MQ=255) gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGaa < 1:82873/251‑1 (MQ=255) ggtccgcgGGAGAAAAATAGCACCAGCGCCATCGCGCGTAAATTGCGTTCTTTGCGTAATGACGGTCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAGCTCGG‑TGATGGGGCGCAGTGAGGTGGCGCGTAAGCAAAAC‑GCCAATTTCCTCGTTTCGATTCACGCTGATGCCGCACCGAGCCGCAGTACGACTGGCGCTTCCGTATGAGTGCTCTCTAACCGTCGCGCCAACAATGaaa < 2:23988/246‑1 (MQ=255) ggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAAt > 1:459622/1‑251 (MQ=255) gagaAAAATGTCACCCTCGCCATCGCGCGTAACTTGCGCACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGCGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAACCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAgctg > 1:211202/1‑251 (MQ=255) tGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTTAAATGGCCAGCTGGCTGGagc > 2:219893/1‑251 (MQ=255) cACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGT‑TGATGGGGCGCAGTGATGTGGCACGGAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCGCGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTGCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGTagcagc < 2:328248/251‑1 (MQ=255) cACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcagc > 1:458639/1‑251 (MQ=255) aCCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcagc > 1:382913/1‑250 (MQ=255) tccatcgcGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACgaga > 2:142530/2‑250 (MQ=255) atcgcTCGTAAATTGCGTACCTTGCTCAATGACGACCCGAT‑GATCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACTTAAGCAAAAC‑GCCAATGTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACGGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGTGCCAACAGTGAAATGTCCATCTGGCTGGGGCAGCACGAGAAACa < 2:530782/251‑1 (MQ=255) cgcgcgTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGGCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGt < 2:194302/251‑1 (MQ=255) gcgTAAATTGCTTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATTGCCAGCTGGCTGGAGCAGCACGAGAATCAGTCgg < 2:47201/251‑1 (MQ=255) gTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg > 1:293549/1‑249 (MQ=255) tAAATTGCGTCCTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGGTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTTCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAg < 2:330288/207‑1 (MQ=255) tAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAg > 1:330288/1‑207 (MQ=255) aaTTGCGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGCGAAACAGTCGGAgctg > 1:260731/1‑251 (MQ=255) cGTACTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtggg > 1:274807/1‑251 (MQ=255) aCTTTGCTCAATGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg < 1:444990/250‑1 (MQ=255) ttttGCTCAATGACGATCCTCT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCCAGG‑TGATGGGGCGATGTGATGTGGCACGTGAGCAAAAC‑GACAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCACAGTGAGACTGGCGATTCAATATGGGTGCACTCTACCCGTCGCGACAACAGTTGAATGGCCAGCTGGCTGGCGCCGCAAgagacaaaataggtgctgctggctggg > 2:294684/2‑228 (MQ=255) aaTGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGa < 2:382913/251‑1 (MQ=255) aTGACGATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGAGGGTg > 1:119970/1‑249 (MQ=255) gATCCGAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtttccggggaatgacga > 1:19643/1‑234 (MQ=255) gAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGCGCTGCTGGGTGGGGCGGGGGGTGTGCTGGCgg > 1:479566/1‑250 (MQ=255) gAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGGTGTGCTGGCgg > 2:523693/1‑250 (MQ=255) gAT‑GTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGATCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCg > 1:328363/1‑250 (MQ=255) | GGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGAT‑GTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGG‑TGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAC‑GCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGA > NC_000913/4396532‑4397016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |