Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,552	G→A	F172F (TTC→TTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,552	0	G	A	100.0%	179.5 / NA	57	F172F (TTC→TTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

AGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTT  >  NC_000913/4393311‑4393785                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           aGATAAGCTGGCCGTCATCGCAACCGAAAATACTGATTACCATTAACGGACGCATCTATTCCGGGATCGCAAATTCAAGTTCGATGGTGAGATAAGAGATACCGCGGCGAGCATAGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGACATACAGGCCAAGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCAGCATATCGACCAGCACTTCGCCTAAAAAAacgaacga                                                                                                                                                                                                                                    >  2:337373/1‑250 (MQ=255)       gCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGcc                                                                                                                                                                                                                             <  1:122723/251‑1 (MQ=255)          ggCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTGCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTc                                                                                                                                                                                                                           <  1:340446/250‑1 (MQ=255)               tCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGAtt                                                                                                                                                                                                                     >  1:185059/1‑251 (MQ=255)               tCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGACCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCACATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGAtt                                                                                                                                                                                                                     >  1:306093/1‑251 (MQ=255)                       aCCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATaa                                                                                                                                                                                                             <  2:206005/251‑1 (MQ=255)                         cGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCACCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGCATATGGCTCGACGATGGCACCGGTCGGCCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATa                                                                                                                                                                                                              >  1:320257/1‑248 (MQ=255)                             aagaCGGTTTCCCATTAACGGTCGCTACTCTTCCGGGTGCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCTGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAagtgagt                                                                                                                                                                                                       <  2:404373/248‑1 (MQ=255)                                tcgttttCCCATTAACTGTAGCAACTCGTCCGTGAGCGCCCCTGCAAGTGCTATGGTGAGATAAGAGATACAGGGGCGAGCGTCGGTGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGACCACGCATCTGCCGCATCCGTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATGCGCCTAAAAAAAAGGACGAACCTGCTGCGCGATTGAGGATAAGTGAGTGc                                                                                                                                                                                                     <  2:480274/246‑1 (MQ=255)                                                     cAACTATTCCGGGATCGAACCTTCAAGTTAGATGGTGAGATAAGAAATACAGCGGCGAGCATCGCCGGCATATGGCCCGACGATGGCACCGGTCGGGCAAATCGCCATACAGGCCCCGCCTTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGCATAACGACCAGCAATTCGCCTAAAAAAAAGAACCAACCGGCCTCGCGATTGAGGATAAGTGAGGACTTACCTGTCCAGCCGAcccccg                                                                                                                                                                               >  2:450373/1‑249 (MQ=255)                                                                ggATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCt                                                                                                                                                                   >  2:405412/1‑251 (MQ=255)                                                                   tCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAAc                                                                                                                                                                >  1:530582/1‑251 (MQ=255)                                                                     gCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCAGCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAATTTGTCATACAGGCCACGCATTTGCCGCAGCCTTCCTCGACTGGTTGATCCATGGGCAGCGGAATTTCTTCCAGCAATTCTGCTAATAAAAAGAACGAACCGGCCTCGCAATGGGGAATAATTGAGTGCGTACCGGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACgg                                                                                                                                                              <  1:515455/251‑1 (MQ=255)                                                                      ccccTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGATCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGGGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACggg                                                                                                                                                             >  2:469520/1‑251 (MQ=255)                                                                        ccTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGgcg                                                                                                                                                           <  1:228305/251‑1 (MQ=255)                                                                           tCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCt                                                                                                                                                         <  2:458069/250‑1 (MQ=255)                                                                             aaGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGGATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATAAAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCCAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCgc                                                                                                                                                      >  1:341994/1‑250 (MQ=255)                                                                                ttCGATGGTGAGATAAGCGATACAGCGGCGAGCATCGACGGAATATGGCTCGACGATGGCACCGGGCGGGCAACTCGTCATACAGGACATGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAAGGGCAGAGGACTATCAACCAGCAATTCGCCTAACACAAAGAACGAACCGGCCTAGCGGATGAGGATAAGGGAGTGATTACCTGACCAGCCGAGCCCGG‑CTTTTTCCGCCAACAGGCGCTCgaga                                                                                                                                                    >  2:500880/1‑250 (MQ=255)                                                                                    aTGGTTAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGTCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCCGAATATCGTCCAGCAATTCGTCGAAAAAAAAGAACGAACCGGCCTCGCGATGGAGGATAAGTGAGTTCTTCCCTGGCCATCCTTGCCCGG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAgg                                                                                                                                               <  1:502882/251‑1 (MQ=255)                                                                                    aTGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAgg                                                                                                                                               >  1:206661/1‑251 (MQ=255)                                                                                      ggTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCGCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGgcg                                                                                                                                             <  2:144866/251‑1 (MQ=255)                                                                                             aaaGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATc                                                                                                                                      <  2:134724/250‑1 (MQ=255)                                                                                                 agaTACAGCGGCGCGCATCGAAGAAATATGGCTAGACGATGCCACCAGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGACTGATCCACGGGCAGCGGAATATCTACCAGCAATTCACCTAAAAAAAAGCACGAACCGGCCTCGCGATTGAGGATAAGTTAGTGCTTAACTGTCCCACCGAGCAAAGACTTTTT‑ACCTAACGGGCGCTCGAGAATAggagaagacgcgac                                                                                                                                   >  2:450976/1‑238 (MQ=255)                                                                                                   aTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACaaa                                                                                                                                <  1:525205/251‑1 (MQ=255)                                                                                                     aCAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGTCAAAc                                                                                                                               <  1:166046/250‑1 (MQ=255)                                                                                                       aGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATCGGCGCAGAATCGACAAACGGt                                                                                                                            >  2:145779/1‑251 (MQ=255)                                                                                                          ggcgAGCATCGACGGGATATGGCTCGTCGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCAACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGGGCCCCG‑CTTTTTCAGCTAACGGACGCTCGAGAATAGGCGCAGAATCGACAAACgggct                                                                                                                          >  2:450760/1‑247 (MQ=255)                                                                                                                        gTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAac                                                                                                           >  2:360005/1‑251 (MQ=255)                                                                                                                              ggctcgccataggaaCCGGTCAGGCACATCGGCCTACAGGCCACGCATCTGCCGAAACCTTCCTCGACTGGTTGCTCCACGGACAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTACTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGTATCGGCGCTGAATCGACAAACGATATAAAATTCAGCGAAaccaaatg                                                                                                     >  2:520875/15‑245 (MQ=255)                                                                                                                                cTCGACAATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGtt                                                                                                   >  1:57950/1‑251 (MQ=255)                                                                                                                                  cgacgaTGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATGCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCGAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGGCTAAAATTCAGCGAAACACAATGTTg                                                                                                  <  2:516690/250‑1 (MQ=255)                                                                                                                                      gaTGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGaa                                                                                             <  1:170719/251‑1 (MQ=255)                                                                                                                                      gaTGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGa                                                                                              >  2:379946/1‑250 (MQ=255)                                                                                                                                         ggCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGaaac                                                                                           >  1:117114/1‑248 (MQ=255)                                                                                                                                         ggCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAatc                                                                                           <  1:383348/250‑1 (MQ=255)                                                                                                                                            aCCGGTCGGGCAAATCGTCATACAGGACACGCCTTTGCCGCATCCTTACTCGACTGGTTGATCAACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATCGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCAtct                                                                                       >  2:321167/1‑251 (MQ=255)                                                                                                                                             ccGGTCGAGCAACTCGACATACAGCCCACGCATTTGCCGCATCATTCCTCGACTGGTTGATCCACGGGCAGAGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGCGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCCGAATCGACACACGGTCTAAAATTCAGCGAAACACAATGATGCTGAATAAtctc                                                                                      >  2:540271/1‑251 (MQ=255)                                                                                                                                                   gggCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAg                                                                                <  2:176666/251‑1 (MQ=255)                                                                                                                                                       aaaTCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCttt                                                                            <  2:206661/251‑1 (MQ=255)                                                                                                                                                           cgtcttccgggCCACGCATTTGCCGCCTCCCTCCTCGACTGGTTGATCCACGCGCAGCGCAATATCGACCTGCAATTCTCCTAAAAAAAAGTACGAACCCGCCTCGCGATTGAGGATAAGTGAGTTCTTACCTGCCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTATAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGa                                                                        <  2:103044/242‑1 (MQ=255)                                                                                                                                                                  cAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGTCAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGtt                                                                 <  2:233558/251‑1 (MQ=255)                                                                                                                                                                             tttGCCGCATCCTTCCTCGACTGGATGATCCACGGGCCGCGGAATATCGCCCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACACACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGa                                                          >  2:36244/1‑247 (MQ=255)                                                                                                                                                                               tGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCAAGCCGAGCCCAG‑CTTTTTCAGATAACGGGCGCTCGAGAATAGGAGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCCCGCCCAGCTTTTTGAGTCGGTTgcggcagaggttg                                                    >  2:195816/1‑241 (MQ=255)                                                                                                                                                                                 ccGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTtgtg                                                  <  1:321167/251‑1 (MQ=255)                                                                                                                                                                                            ctCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGc                                       >  1:292410/1‑251 (MQ=255)                                                                                                                                                                                               gACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTc                                                                                                                           >  2:94087/1‑164 (MQ=255)                                                                                                                                                                                               gACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTc                                                                                                                           <  1:94087/164‑1 (MQ=255)                                                                                                                                                                                                            ccGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGTGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCt                       <  1:500880/250‑1 (MQ=255)                                                                                                                                                                                                                    cGGAATATCGCCCAGCAATTCGCCTAAAAAAAAGAACGAACCCGCCTCGCGATTGAGGATAAGGGAGTGCTAACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAACCACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGc               <  2:179948/251‑1 (MQ=255)                                                                                                                                                                                                                    cGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCTCAGAATCGACAAACGGGCTAAAATTGAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGTTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGc               <  1:358596/251‑1 (MQ=255)                                                                                                                                                                                                                     ggAATATCGACCAGCAATTCGCCTAAAAAAAAGCACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGCCAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACCTAGcc              >  2:299071/1‑251 (MQ=255)                                                                                                                                                                                                                          atCGAGCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTGTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGGTAACATAGCCGAGt          <  2:165513/250‑1 (MQ=255)                                                                                                                                                                                                                          atCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGt          <  1:524428/250‑1 (MQ=255)                                                                                                                                                                                                                            cGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTg       <  2:105052/251‑1 (MQ=255)                                                                                                                                                                                                                              aCCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGCGTTTggg     >  1:282385/1‑251 (MQ=255)                                                                                                                                                                                                                                 aGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGGGTTTGGGGtt  >  1:518666/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAatcatc                                                                                        <  1:233036/157‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           AGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAG‑CTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTT  >  NC_000913/4393311‑4393785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑