Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,347	T→A	intergenic (+138/‑131)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,347	0	T	A	100.0%	71.5 / NA	22	intergenic (+138/‑131)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/12);  total (10/12)

GCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCT  >  NC_000913/4215102‑4215584                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTTCCAGATCACGACATACGATCTACGGCACATGAATCCAACGCTGGAGTAATCTTCTGTGATAGTCGATCGTTTAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTTCCTTTTTTATTTCCGAAACGTTCCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTataatt                                                                                                                                                                                                                                          <  1:539972/251‑1 (MQ=255)             cgTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCACAGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCTGGTGCCTTTTTTATTTCCGAAACGTACCTCAACAGGTGAATAAATTTTATTCATATTGTTATCACCAAGTTATCAAATATTTATAATTAAAATGGAAAtt                                                                                                                                                                                                                              >  2:470381/2‑251 (MQ=255)                      aaTTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGAt                                                                                                                                                                                                                     <  1:94499/251‑1 (MQ=255)                         ttACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGAttt                                                                                                                                                                                                                   <  1:112373/250‑1 (MQ=255)                            cTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCCTACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGc                                                                                                                                                                                                                >  2:363516/1‑250 (MQ=255)                              gTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCa                                                                                                                                                                                                               <  1:338840/249‑1 (MQ=255)                                        tggctaacccaTTACGTCTACCAGCTCACGCCATACGATCAACGCAACATGAATCCAACGCTGGACTCCTCTCCTGTGCCAGTCGATCGTTACGCGCTTCAGCACCTTACCTCCGCCACCTTCGGGTGCCTTTTTTATTTCCGAAACCTACCTCAGCAGCCGCATAACTTTTATTACTATTTTTATCACCACATTCTCAAGTATTTATAATTAATATGGAAATTGTTTTTGCTTTTGCATTATAAATGagt                                                                                                                                                                                                   >  2:262502/11‑251 (MQ=255)                                            tCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGATAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTAACTAAGCAGGGGAATAAGTTTTATTCTGATTGTTATCAACAAGTTCTCAAGAATTTATAATTAAACTGGACTTTGTTTTTGATTTTGCATTTTAAATGAGTAGTc                                                                                                                                                                                               >  2:87541/1‑251 (MQ=255)                                               aCTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTa                                                                                                                                                                                            <  1:352227/251‑1 (MQ=255)                                                cTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCtt                                                                                                                                                                                             >  1:271724/1‑249 (MQ=255)                                                cTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCtt                                                                                                                                                                                             >  1:323712/1‑249 (MQ=255)                                                                                                              cttctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATAAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGATGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCAt                                                                                                                             >  1:460107/1‑251 (MQ=255)                                                                                                                     gATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACgagg                                                                                                                      >  1:167018/1‑251 (MQ=255)                                                                                                                                gTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAATCGTACCTCATCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATGTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCTCAGTGGGGAAGGTTTCGGAGCCATGACGAGGAGCGGCACGAt                                                                                                           <  1:202398/251‑1 (MQ=255)                                                                                                                                                          ggCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGGTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATg                                                                                 <  2:323712/251‑1 (MQ=255)                                                                                                                                                                           tttttATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACaa                                                                  <  2:167018/249‑1 (MQ=255)                                                                                                                                                                              ttATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACCGGCTTTCACAAGGcc                                                              <  1:261984/250‑1 (MQ=255)                                                                                                                                                                                                                   tATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGaa                         <  2:452413/250‑1 (MQ=255)                                                                                                                                                                                                                      tCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCg                      <  1:171631/250‑1 (MQ=255)                                                                                                                                                                                                                                aTCAACAAGTTATCAAGTATTTATAATTAAAATGGAACTTGTGTTTGATTTTGCATTTTAAATGAGTATTCTTAGTTGTGCTGAATGAAAAGAGCACAACGATCCTTCGTTCACAGTTGGGAAGTTTTCGGATACATGACGAGGAGCTGAACGATGACTGAACAAGCAACAACAACAGATGACCTGGCTTTCACAAGGCCGTATGGCGCGCAGGCGAAGCAAATTCTTCCTGCCGAAGCGATGGAATTTct           >  2:519571/1‑251 (MQ=255)                                                                                                                                                                                                                                          tATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCt  <  1:485745/250‑1 (MQ=255)                                                                                                                                                                                                                                            tCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACt                                                                             <  1:497658/173‑1 (MQ=255)                                                                                                                                                                                                                                            tCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACt                                                                             >  2:497658/1‑173 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCT  >  NC_000913/4215102‑4215584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑