Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	363,292	G→T	S1005* (TCG→TAG)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	363,292	0	G	T	100.0%	158.8 / NA	43	S1005* (TCG→TAG)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/18);  total (25/18)

TTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACAT  >  NC_000913/363067‑363530                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               ttGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGCCACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTACAGGAggcgtc                                                                                                                                                                                                                       >  2:138709/1‑251 (MQ=255)          tGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGCACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAAt                                                                                                                                                                                                              >  1:79292/1‑251 (MQ=255)                         aaaagCGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAAcc                                                                                                                                                                                               <  1:35347/251‑1 (MQ=255)                             aCGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCg                                                                                                                                                                                           <  1:30234/251‑1 (MQ=255)                                      aGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGc                                                                                                                                                                                  <  2:28012/251‑1 (MQ=255)                                            ccATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTGTTTTAAATAGTACAGAATGGATTTCCTTATGCGAAATACGGGCAGACAGGGCCTGACCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGGCGTCGCCGCCAATCCCCATATGGAAACCGTCGATATTCAGCCAtgtg                                                                                                                                                                            <  2:24415/251‑1 (MQ=255)                                                    aaaaaGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCttcttc                                                                                                                                                                    <  1:138709/251‑1 (MQ=255)                                                         gTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGt                                                                                                                                                               <  1:133930/251‑1 (MQ=255)                                                          tAAAAGACAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAAcc                                                                                                                                                                                               >  2:95821/1‑218 (MQ=255)                                                          tAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAAcc                                                                                                                                                                                               <  1:95821/218‑1 (MQ=255)                                                          tAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTg                                                                                                                                                              >  1:367865/1‑251 (MQ=255)                                                                  aaagaaTAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTCCATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCACCTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCCCCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgat                                                                                                                                                      <  1:390002/251‑1 (MQ=255)                                                                      aaTAAACCGAACATCCAACAGTTTGTGTTTTTTACATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCATACCCGGTTATTATTATTTTTGCAACCAGACCAACTGGTAATGGTGGCGAACGACGCTCAGCTGGAATTCCGCCTATAGTGACGGGCTCCAGGAGACGTCGCCACGAATCTCCATATGCAAACCGTCGTTATTCAGCCATGTGCCTTCTTCCGCGAgcagcaaattgcc                                                                                                                                                  >  2:95883/1‑244 (MQ=255)                                                                              gAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTgg                                                                                                                                          >  1:88679/1‑251 (MQ=255)                                                                                      aaaaGTTTGTGTAATTTAAATAGTCCATAATGGATTTCCTTACGCGAAATACGGGCAGTCATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGAAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGATCCAGGAGTCGTCGCCACCAATCCCCATCTGGAAACAGTCGATATTCAGCCATGAGACTTCTCCCGCGTGCAGCAGATGGCGAAGGCTGGTTTCCATc                                                                                                                                  >  2:299194/1‑251 (MQ=255)                                                                                          gTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGTTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGcc                                                                                                                                                                                                                    <  2:532842/165‑1 (MQ=255)                                                                                          gTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGcc                                                                                                                                                                                                                    >  1:532842/1‑165 (MQ=255)                                                                                                        aaTAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGt                                                                                                                  >  1:416120/1‑249 (MQ=255)                                                                                                          tAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCTCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGACTTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGACACCGTCGATATTCCGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGATGTTGACTGTAGCg                                                                                                              >  2:7685/1‑251 (MQ=255)                                                                                                               cATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCCGTTGCTGTTGACTGTAGCGGCTg                                                                                                          >  2:157743/1‑250 (MQ=255)                                                                                                               cATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGGAGCGGCTgt                                                                                                         >  2:118529/1‑250 (MQ=255)                                                                                                                      gATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGaa                                                                                                  <  1:7685/251‑1 (MQ=255)                                                                                                                        tttCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACt                                                                                                >  1:26332/1‑251 (MQ=255)                                                                                                                        tttCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGACGCTCAGCTGGAATTCCGCCTATACTGCCGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGCCTGCAGCGGCTGATGTTGAACt                                                                                                >  2:173732/1‑251 (MQ=255)                                                                                                                           ccTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTgg                                                                                              <  2:367865/250‑1 (MQ=255)                                                                                                                            cTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGa                                                                                             >  1:248459/1‑250 (MQ=255)                                                                                                                              tACGCGAAATACGGGCAGACATGGCTTGCCCTGTTATTATTATTTTTGACACCAGACCAACTGGTAAGGGTAGCGACCGGCGCGCAGCTGGAATTCCGCCTATACTGACGTGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGGTGATCTGGAAg                                                                                           <  2:79292/250‑1 (MQ=255)                                                                                                                                          gggCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACt                                                                              <  1:157743/251‑1 (MQ=255)                                                                                                                                           ggCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTg                                                                             >  1:427327/1‑251 (MQ=255)                                                                                                                                              aGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTTATGTTGAACTGGAAGTCGCCGCGCCACTGgtg                                                                          <  1:524502/251‑1 (MQ=255)                                                                                                                                                  aTGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCGCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTggg                                                                      <  1:440057/251‑1 (MQ=255)                                                                                                                                                   tGGCCTGCCCGGTTATTATTATTTTTGACACAAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGgttggggc                                                                     >  2:141408/1‑245 (MQ=255)                                                                                                                                                          cccGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaatt                                                              >  1:267720/1‑251 (MQ=255)                                                                                                                                                            cGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATaattc                                                             >  1:412468/1‑250 (MQ=255)                                                                                                                                                                    attattTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCGCCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTcgcg                                                     <  1:84332/250‑1 (MQ=255)                                                                                                                                                                       attTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTc                                                 >  1:525191/1‑251 (MQ=255)                                                                                                                                                                                      ccAACTGGTCATGGTAGCGACAAGCGAACCGCTGCCATTCCGCCTACACTGACGGGCTCCAGGAGTCCTCGACACCAATCCCCATCTGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGCCTGTAGCGGCTGATGTTGGACTGGCAGAAGCCGCGCCACTGGTGTGGGCACTAATTCAATTCGCGCGTCCAGCAGCGCAGACCg                                  >  2:231328/1‑251 (MQ=255)                                                                                                                                                                                         aCTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGttt                               >  1:43562/1‑251 (MQ=255)                                                                                                                                                                                                aTGGTAGCGACCGGCGCTCAGCTGGAATTCCTCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCACTCCCCCTATGGACACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCCGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTTTTGGCCATCATTCCATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCg                        >  2:60282/1‑251 (MQ=255)                                                                                                                                                                                                     ggCGACCGGCGCTCAGCTGGAATCCCGCCTATACTGACGGGCTCCAGGAGTCGCCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAg                   <  2:525191/250‑1 (MQ=255)                                                                                                                                                                                                        gACCGGCGCTCAGCTGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACg                >  1:160726/1‑251 (MQ=255)                                                                                                                                                                                                           cGGCGCTCAGCTGGAATTCCGCCTATACTGCCGGGCTCCAGGAGGCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTAc             <  2:88679/251‑1 (MQ=255)                                                                                                                                                                                                                      tGGAATTCCGCCTATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTACACAt  >  2:330596/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               TTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTCGCTCGGGAAGACGTACGGGGTATACAT  >  NC_000913/363067‑363530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑