Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,525	G→A	A78T (GCC→ACC)	rbsK →	ribokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,525	0	G	A	100.0%	255.0 / NA	73	A78T (GCC→ACC)	rbsK	ribokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

GGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATA  >  NC_000913/3937281‑3937771                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ggACATCCCGAAGATGCAAAACGCAGGCAGCCTCGTTGTTTTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTGCTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCGGCTGGGCGTGGCGGTGCGTATATCGCGGTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTg                                                                                                                                                                                                                                                  <  2:521328/251‑1 (MQ=255)       cccGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAAc                                                                                                                                                                                                                                             <  2:386981/250‑1 (MQ=255)            atatGCAAAACGCAGGCAGCCTCGTTGTTCGTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGAGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGat                                                                                                                                                                                                                                       <  1:21464/251‑1 (MQ=255)              atGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGatat                                                                                                                                                                                                                                     <  1:199253/251‑1 (MQ=255)                gCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATAtt                                                                                                                                                                                                                                    <  1:259356/250‑1 (MQ=255)                gCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAAGATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATAtt                                                                                                                                                                                                                                    <  2:250758/250‑1 (MQ=255)                  aaaaCGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACt                                                                                                                                                                                                                                 <  1:499026/251‑1 (MQ=255)                   aaaCGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTc                                                                                                                                                                                                                                >  1:38626/1‑251 (MQ=255)                              gcctcttgtgtCTTGGCAGCATTAATTCTGTCCACATTCTTAATCTTCAATCGTTTCCTCCTCCAGGCTAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAATGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGTAATGGTGAGAGCGTTCGCCAGCAGCTCACCACTGAGAACATTGATAGTACTCCGGCCAGCGTg                                                                                                                                                                                                                     <  2:523855/241‑1 (MQ=255)                                    ttgttATTGGCAGCATTAATGCTGACCACATTCTTACTCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGAGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGGTCaac                                                                                                                                                                                                               >  2:425733/1‑250 (MQ=255)                                      gttCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAg                                                                                                                                                                                                              <  1:149137/250‑1 (MQ=255)                                           tGGCAGCATTAATTCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGAGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGAGATCAAAGGCgcat                                                                                                                                                                                                        >  2:469338/1‑248 (MQ=255)                                           tGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAAt                                                                                                                                                                                                        >  1:372823/1‑251 (MQ=255)                                              cagcaTTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCa                                                                                                                                                                                                      >  1:130674/1‑250 (MQ=255)                                                gcaTTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAAc                                                                                                                                                                                                    >  1:275251/1‑250 (MQ=255)                                                  aTTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAgg                                                                                                                                                                                                 >  1:317713/1‑251 (MQ=255)                                                    tAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTAGCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGgt                                                                                                                                                                                                <  2:93400/250‑1 (MQ=255)                                                      aTGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGtgtg                                                                                                                                                                                             <  1:71138/251‑1 (MQ=255)                                                                caTTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTCACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGAttt                                                                                                                                                                                   >  1:136227/1‑251 (MQ=255)                                                                 actCTTAATCTTCAAGTTTTTTCTACTCCAGGCGAAACCGTAACCGGTAACCACTATAAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGGTTATTGCCTGTACGTGTGATGACAGCATTGGTGAGAGCGTTCGCCTGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGAtttt                                                                                                                                                                                  <  1:157296/249‑1 (MQ=255)                                                                     ttAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGtt                                                                                                                                                                              >  1:63872/1‑251 (MQ=255)                                                                       aaTCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTa                                                                                                                                                                             >  1:205457/1‑250 (MQ=255)                                                                         tCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTCAACACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATAAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATg                                                                                                                                                                          >  1:518747/1‑251 (MQ=255)                                                                          cTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGAGTGGCGCTGATTTTTGTTAATgg                                                                                                                                                                         >  2:383950/1‑251 (MQ=255)                                                                          cTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGGGAGAGTATCGCGTTTATCGCCTGTACGGGTGATGACAGCATTGGTGCGAGCGTTCGCAAGCAGCTCACCACTGATAGCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGGGTGGCGCTGATTTTTGTTAATgg                                                                                                                                                                         >  1:113360/1‑251 (MQ=255)                                                                                         aCTCCAGGCGAAACCGTAACCGGTACCCACGATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGAACAGAGGCGAATCGACAGGTATGGCGCTGCTTTTTGTTAATGGCGACGGTGAGaaagt                                                                                                                                                          >  2:357256/1‑248 (MQ=255)                                                                                         aCTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGGTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTTGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGCTAACATTGTTATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGGGTGGCGCTGATTTTTGTTCATGGCGAAGGTGAGAATGt                                                                                                                                                          >  2:323653/1‑251 (MQ=255)                                                                                              aGGCTAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCg                                                                                                                                                     <  2:140894/251‑1 (MQ=255)                                                                                                  gAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTAt                                                                                                                                                 >  1:230850/1‑251 (MQ=255)                                                                                                  gAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTAt                                                                                                                                                 >  1:366854/1‑251 (MQ=255)                                                                                                   aaaCCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTAtt                                                                                                                                                >  1:321273/1‑251 (MQ=255)                                                                                                       cGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATg                                                                                                                                            <  1:316643/251‑1 (MQ=255)                                                                                                       cGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATg                                                                                                                                            <  1:319154/251‑1 (MQ=255)                                                                                                            ccGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGgc                                                                                                                                       <  2:412244/251‑1 (MQ=255)                                                                                                              ggTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGgcgc                                                                                                                                     >  1:161842/1‑251 (MQ=255)                                                                                                                   ccACTATCAGGTTGCCTTTGGCGGCCAAGGCGCGAATCAGGCTGTGGCTGCTGGGCCTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCACTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCACCGCGATCCAAGGCGAATCAACCGGCGGGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGCATTCATGCCGGCGCTAAtg                                                                                                                                >  2:540951/1‑251 (MQ=255)                                                                                                                     aCTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCTAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAAtgct                                                                                                                              <  2:87297/251‑1 (MQ=255)                                                                                                                                   tttGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCCTCGGTATTCATGCCGGCGCTAATGCTGACCTTTCCCCgtc                                                                                                                >  2:463171/1‑249 (MQ=255)                                                                                                                                         ggcAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTTCCCTTTCCCCGGCGCtggt                                                                                                          <  2:474223/251‑1 (MQ=255)                                                                                                                                               ggCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTAGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAgc                                                                                                    >  1:484130/1‑251 (MQ=255)                                                                                                                                                    gAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATAGTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCAGCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAAc                                                                                               <  2:443009/251‑1 (MQ=255)                                                                                                                                                        cAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGa                                                                                           <  2:180542/251‑1 (MQ=255)                                                                                                                                                                ggCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                    <  1:125264/250‑1 (MQ=255)                                                                                                                                                                  cggctgGGCGTAGCGGTGCGAAGAGCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCGGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGcc                                                                                 <  2:382893/249‑1 (MQ=255)                                                                                                                                                                   tgctgGGCGTAGCGGTGAGAATATCGAGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACCTTGCTATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGACGGGGAGAATGTCTTCGGTATTCATGCCGGCGCTCATGCTGCCCTTTCCCCGGCGCTGGTGGAAGTGCCACGTGcgcggatggca                                                                                 >  2:230234/1‑243 (MQ=255)                                                                                                                                                                       gggCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAAGGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCTTATTGCCAAcgc                                                                            <  2:19404/251‑1 (MQ=255)                                                                                                                                                                        ggCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAAcgcg                                                                           <  1:469338/251‑1 (MQ=255)                                                                                                                                                                           gTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATAAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTcc                                                                        >  2:375794/1‑250 (MQ=255)                                                                                                                                                                            tAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGTTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGAATTGCCAACGCGTc                                                                         >  2:455314/1‑249 (MQ=255)                                                                                                                                                                            tAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTTCCAACGCGTc                                                                         >  1:156041/1‑249 (MQ=255)                                                                                                                                                                            tAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTc                                                                         >  2:2525/1‑249 (MQ=255)                                                                                                                                                                             aGCGGTGCGAATATCGCGTTTATTGCCTGAACGGGTGATGACAGCATTGGTGAGAGAGTTCGCCCGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGCATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGc                                                                      >  2:520856/1‑251 (MQ=255)                                                                                                                                                                              gCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                     <  2:130674/251‑1 (MQ=255)                                                                                                                                                                                   gCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCattat                                                                 <  1:36821/250‑1 (MQ=255)                                                                                                                                                                                    cGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGAGTTCGCCAGCAGCTCAACACTGCTAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGGGAGAATGTCATCGGTATTAATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCCACGTGAGCGTATTGCCAACGCGCCAGCattatt                                                                >  2:521731/1‑250 (MQ=255)                                                                                                                                                                                             cgTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                      <  1:397684/251‑1 (MQ=255)                                                                                                                                                                                                     gCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcac                                               <  1:383950/250‑1 (MQ=255)                                                                                                                                                                                                      ccTGTACGGTTGATGACAGCATTGGTGAGAGCGTTCGCCAGCATCTCACCACTTATATAATTGATATTACTGCGGTCAGCGTGATCAAAGGCGAATCAACAGGTTTGGCGCTGATTTTTGTTAATGGCTAAGGTGAGAATGTCATCGTTATTCATGCGGTTTCTAATGCTGCCCTTGCCGCGGGGCTTGTGGAGGCGCAACGTGAGCGTTTTGCCGGCGGGTCAGCAttattattgtggcttgtatcacc                                              <  2:517272/250‑18 (MQ=255)                                                                                                                                                                                                               gTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACAACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGAGAGAATGTCATAGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATAACCACTCGAAAgg                                    >  2:517633/1‑250 (MQ=255)                                                                                                                                                                                                               gTGATGACAGCATTGGTGAGAGCGTGCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGGCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTGTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAGCGCGTCAGCATTATTAATGCAGCTGGAATCACCACCCGAAAgt                                    <  2:180953/251‑1 (MQ=255)                                                                                                                                                                                                                 gatgaCAGCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGGCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgtgt                                  <  2:317713/251‑1 (MQ=255)                                                                                                                                                                                                                 gacgaCAGCATTGGTGAGAGCGTTCTACAGCATCTCACCGCTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACATGTTGGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGTTATTCATGCCGGCTCTAATGCTGCTCTTTCCCCGGCGCTGGTGTAAGCGCAACGTGATCTTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgtgt                                  <  1:521731/248‑1 (MQ=255)                                                                                                                                                                                                                        gCATTGGTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGc                            >  1:475547/1‑250 (MQ=255)                                                                                                                                                                                                                             ggTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAAGGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggc                      <  1:540951/251‑1 (MQ=255)                                                                                                                                                                                                                              gTGAGAGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggcg                     >  1:371392/1‑251 (MQ=255)                                                                                                                                                                                                                                gagaGCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTGCTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGTTGAGAATGTCATCGGTATTCATGCCGTCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGGCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGAGGCGa                    >  1:368482/1‑250 (MQ=255)                                                                                                                                                                                                                                    gCGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATc               <  1:463171/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcg                        >  2:418911/1‑241 (MQ=255)                                                                                                                                                                                                                                     cGTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcg                        <  1:418911/241‑1 (MQ=255)                                                                                                                                                                                                                                      gTTCGCCAGCAGCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATGTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGGTAATCGc             <  2:155042/251‑1 (MQ=255)                                                                                                                                                                                                                                            cagcagCTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCa       <  1:111255/251‑1 (MQ=255)                                                                                                                                                                                                                                                agcTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAAt   <  1:2525/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gcTCACCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCTCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATa  <  2:161842/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAATGCTGACCACATTCTTAATCTTCAATCTTTTCCTACTCCAGGCGAAACCGTAACCGGTAACCACTATCAGGTTGCATTTGGCGGCAAAGGCGCGAATCAGGCTGTGGCTGCTGGGCGTAGCGGTGCGAATATCGCGTTTATTGCCTGTACGGGTGATGACAGCATTGGTGAGAGCGTTCGCCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATA  >  NC_000913/3937281‑3937771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑