Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,907,139 G→T A147E (GCG→GAG)  phoU ← negative regulator of the pho regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,907,1390GT98.8% 292.3 / NA 83A147E (GCG→GAG) phoUnegative regulator of the pho regulon
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (51/31);  minor base A (1/0);  total (52/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCGCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCGCACGCAGGCTTCATCGATCGCCAC  >  NC_000913/3906894‑3907383
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
cATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAAc                                                                                                                                                                                                                                                 <  1:72715/251‑1 (MQ=255)
 aTCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGCATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAAcg                                                                                                                                                                                                                                                >  1:441282/1‑251 (MQ=255)
 aTCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAAcg                                                                                                                                                                                                                                                >  2:183386/1‑251 (MQ=255)
     ccACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTc                                                                                                                                                                                                                                            >  1:285340/1‑251 (MQ=255)
       aCCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAACTATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTccc                                                                                                                                                                                                                                          >  1:46285/1‑250 (MQ=255)
          gACGTGACGGAAATCCGGCCGCTTCACGTAGTAGAAGATAAACTCACAAATTTTCTGGCATCTGTCGCCAATCCGTTCGATAGAACGCGTGCAGATCAGCGCAGTATGTACTCTCGTAATGGTACGTGTACCTTCCATCATGTAGATCATCAGTAGACTAACAATTCCATCTTATTCCTGATCGACTTTTTTGTCTTCACGAGAAATGCGTACTGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCa                                                                                                                                                                                                                                       <  1:442910/251‑1 (MQ=255)
                     aaTCCTACCCCTTCCCGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGAACGCGAATCTTCCCTCATGTAGGTCATCAGTTGACGAACCATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTACAGCACGTCGTgcagc                                                                                                                                                                                                                            >  2:156329/1‑251 (MQ=255)
                           gCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCACTACGTTCGATAGAACTCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATTTCCATCCGCTCGACCGCGTCCAGCActtagatcgtaagcgcg                                                                                                                                                                                                                      >  2:461895/1‑235 (MQ=255)
                           gCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCTTCAGTTGACGAACAATTCCTTCGTATTCCTGATCGACTTTTTTATCTTCACGAGAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCAcgt                                                                                                                                                                                                                                    <  1:461895/237‑1 (MQ=255)
                           gCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTgg                                                                                                                                                                                                                      <  1:183386/251‑1 (MQ=255)
                                  cACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTAt                                                                                                                                                                                                               >  1:367979/1‑251 (MQ=255)
                                          agaagaTAAACTCACAAATACTCTGGCAGCTGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGCACGCTCGGAATCGTACGCGAATCCTCCATCATGTAGGTCATCAGTTTACTAACAATACCTTCTTATTCCTGATCGACTTGTTTATCTTCACGATAAATACGTACCGCTTCGTCAAGGTCCATCCGCTCGAACGCGTACAGCACGTCGTGCAGCATCTGGATGGTAGGACGGcc                                                                                                                                                                                                        <  2:271823/250‑1 (MQ=255)
                                            aagaTAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAg                                                                                                                                                                                                     <  1:457353/251‑1 (MQ=255)
                                             agaTAAACTCACAACTATTCTGGCAGCGGTCGCCAATACGTTAGATAGAACGCGCGCAGAACAGCGCAGCAGGTACGCTCGGAATGGTACGAGACTCTTCCATCATGTAGGTCATCAGTTGCCGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTAAATGTCCATCCGCTCGCACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCAAGc                                                                                                                                                                                                    >  2:74493/1‑251 (MQ=255)
                                              gaTAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCg                                                                                                                                                                                                   <  2:27581/251‑1 (MQ=255)
                                                   aCTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAACTACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTcc                                                                                                                                                                                              >  2:520155/1‑251 (MQ=255)
                                                       acaattagtctgggatcGGTCTCCAATACGGTCGAGAGAACGCGCGCATAACATCGCAGTAAGTACGCTCGTAATGGAACGCGAATCTTCCATCATGTAGGTCATCAGTGGACGAACAATACCTTCGTATTCCTGATCGACTTTTGTAGCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGTGGCCAGCACGTGGTGCATCATCTGGATTGTATGACGGCTTAGCGACTCCAGAc                                                                                                                                                                                          <  2:487427/235‑1 (MQ=255)
                                                       acaAATATTCTTGCAGCGGTCGCCAATACGTTCGAGAGAACGCGCGCAGAACAGCGCAGTTAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTGGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACTATAAATACGTACCTCTTCGTCAATGTCCAGCCGCTCGAACGCGTCCAGCGCGTCGTGCCGCATCTGGATGGTATGACGGCCCAGCGGCTCCAGAc                                                                                                                                                                                          <  2:272674/251‑1 (MQ=255)
                                                              ttCTGGCAGAGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTAcc                                                                                                                                                                                     >  2:190722/1‑249 (MQ=255)
                                                               tCTGGCAGCGGTCGCCAATACATTCGATAGAACCCGCGCAGAACACCGCAGTAAGTACGCTCGGAATGTTACGCGAGTCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTAAAGATAAATACGTACCGCTTCGTCAATGACCATACGCACGAACGCGTCCCGCACGTCGTGCAGCATCTGGATGGTGTGACGGCCACGCGACTCCGGCATTACCAGc                                                                                                                                                                                  >  2:335723/1‑251 (MQ=255)
                                                                 tGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAAc                                                                                                                                                                                                                                                 >  2:10796/1‑186 (MQ=255)
                                                                 tGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAAc                                                                                                                                                                                                                                                 <  1:10796/186‑1 (MQ=255)
                                                                 tGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCAc                                                                                                                                                                                                                                      >  2:416963/1‑197 (MQ=255)
                                                                 tGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCAc                                                                                                                                                                                                                                      <  1:416963/197‑1 (MQ=255)
                                                                   gCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACg                                                                                                                                                                              >  1:198890/1‑251 (MQ=255)
                                                                        ggTCGCCAATACGTTCGATAGAATGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGACTCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCCTCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGCCTCCAGACTTACCAGCAACGGCTgg                                                                                                                                                                         >  2:540953/1‑250 (MQ=255)
                                                                            gCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGAtgct                                                                                                                                                                     >  2:111467/1‑251 (MQ=255)
                                                                                                gcgcgcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAgc                                                                                                                                                 <  1:531211/251‑1 (MQ=255)
                                                                                                   cgcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAgcgc                                                                                                                                               >  1:89199/1‑250 (MQ=255)
                                                                                                      agatcAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACTATCAATACCTTCGTATTCCCGATCGACTTTTTTATCTTCACGATAAATACGTCCCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACTAGCACCGGCTGATGCTGCTGGGAGAATTTCTCCTGCGCAGTa                                                                                                                                           <  2:285340/247‑1 (MQ=255)
                                                                                                       gAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCAGCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTAc                                                                                                                                          <  2:142370/251‑1 (MQ=255)
                                                                                                                  taagtaCGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTg                                                                                                                               >  1:38267/1‑251 (MQ=255)
                                                                                                                   aagtaCGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAg                                                                                                                                                                                   <  1:73199/198‑1 (MQ=255)
                                                                                                                   aagtaCGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAg                                                                                                                                                                                   >  2:73199/1‑198 (MQ=255)
                                                                                                                    agtaCGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTAACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAAAGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTc                                                                                                                             >  1:18389/1‑251 (MQ=255)
                                                                                                                        cGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGcc                                                                                                                         <  1:415212/251‑1 (MQ=255)
                                                                                                                         gCTCGGAATGGGACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTCTTCCTGTTCGCGTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGACCGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCACCGGCTGATGATGCTGGGAGAATTTCTCCCGCGCAGTACGGCCGATATTGTCCGcc                                                                                                                         >  2:153309/1‑250 (MQ=255)
                                                                                                                          cTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCAc                                                                                                                       >  2:183271/1‑251 (MQ=255)
                                                                                                                            ctgaatggtacttgaagttgccATCATGTAGGTCATCAGTTGCCGAAAAATACCTTCGTATTCCTGAGCGACTTTTTTACCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCTCGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGGTGAGGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCAc                                                                                                                       <  2:229685/229‑1 (MQ=255)
                                                                                                                             ggAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTc                                                                                                                    >  1:258764/1‑251 (MQ=255)
                                                                                                                              gAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCg                                                                                                                   >  1:177612/1‑251 (MQ=255)
                                                                                                                              gAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGACCGGCAGATTTTGTCCGCCACGTCg                                                                                                                   >  2:376862/1‑251 (MQ=255)
                                                                                                                                aTGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTCCCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGcc                                                                                                                 >  2:111783/1‑251 (MQ=255)
                                                                                                                                  ggTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCa                                                                                                                >  1:413812/1‑250 (MQ=255)
                                                                                                                                  ggTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCa                                                                                                                >  2:470983/1‑250 (MQ=255)
                                                                                                                                   gTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAAt                                                                                                              <  2:367979/251‑1 (MQ=255)
                                                                                                                                   gTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAAt                                                                                                              >  2:6199/1‑251 (MQ=255)
                                                                                                                                                 catcatGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCgg                                                                                                <  1:33219/251‑1 (MQ=255)
                                                                                                                                                    catGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCa                                                                                              >  2:23387/1‑250 (MQ=255)
                                                                                                                                                        tAGGTCATCAGTTGACCAACCATACCTTCGTATTCCTGCTCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGccacggt                                                                                         >  2:480076/1‑245 (MQ=255)
                                                                                                                                                                caGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTg                                                                                  >  1:486508/1‑250 (MQ=255)
                                                                                                                                                                caGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGGCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACGTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGa                                                                                 <  1:533669/251‑1 (MQ=255)
                                                                                                                                                                caGTTGACGAACAATACCTTCGTATTCCTGATCGACTGTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTg                                                                                  <  1:207589/250‑1 (MQ=255)
                                                                                                                                                                 aGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGAt                                                                                >  1:34160/1‑251 (MQ=255)
                                                                                                                                                                  gTTGACGCACAATACCTTCGTATTCCTGATCGCCTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGGTGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCGGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTTATc                                                                               >  2:100983/1‑251 (MQ=255)
                                                                                                                                                                     gACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCAc                                                                             <  1:283676/250‑1 (MQ=255)
                                                                                                                                                                                ccTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCg                                                                 <  1:520155/251‑1 (MQ=255)
                                                                                                                                                                                ccTTCGTATTCATGCTAGACTTTTTTATCTTCACGATCAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCACAGCGACTCCAGACTTACCAGCAACAGCTGATGCTGCTGGGAGACTTTCTCCAGCGCAGTACGGCAGATTTTGTACGCCCAGACGCCAATACACTCCAGCGCGGCAATGGTTTTACTGATCAcactaacacgtcg                                                                 >  2:352343/1‑239 (MQ=255)
                                                                                                                                                                                   gcGTATTCCTGATCGACTTTTTTATCTTCACGATAAATTCGTACCGCTTCGTCAATGTCCATCCGCTCGAGCGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCa                                                               <  2:540291/249‑1 (MQ=255)
                                                                                                                                                                                           cTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGtcgctcg                                                      >  1:394396/1‑251 (MQ=255)
                                                                                                                                                                                           cTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGCCGCAGGtcgctcg                                                      >  1:24357/1‑251 (MQ=255)
                                                                                                                                                                                            tGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGGACGGCAGATTTTGTCCGCCACGTCGCCAATACGCCCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGtcgctcgc                                                     >  2:141134/1‑251 (MQ=255)
                                                                                                                                                                                             gATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATACCCAGTCGCCGGTCGCTCGcc                                                    >  2:420587/1‑251 (MQ=255)
                                                                                                                                                                                              aTCGACTTTTTTATCTTCACGATAAATACGTGCCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCATATGTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTGTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCg                                                   <  2:46285/251‑1 (MQ=255)
                                                                                                                                                                                                        ttATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACg                                         >  1:70696/1‑251 (MQ=255)
                                                                                                                                                                                                        ttATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCCCCAGCTCGGCCATGGTTTTACTGATCACCATACCCAGTCGCAGGTCGCTCGCCGTCGGCTGACg                                         >  2:271253/1‑251 (MQ=255)
                                                                                                                                                                                                         tATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGt                                        >  1:329232/1‑251 (MQ=255)
                                                                                                                                                                                                            cTTCACGATAAATACGTACCGCTTCGTCAATGTAAATCCGCTCGAACAAGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACAGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTAAGGAAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCCGGTCGCTCGCCGTCGGCTGAAGTTTg                                     >  1:501320/1‑251 (MQ=255)
                                                                                                                                                                                                             ttCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTgg                                    >  2:172970/1‑251 (MQ=255)
                                                                                                                                                                                                             ttCACGATAAATACGTACCGCTACGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTgg                                    >  1:423576/1‑251 (MQ=255)
                                                                                                                                                                                                              tCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGATCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGCACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGc                                   >  1:403433/1‑251 (MQ=255)
                                                                                                                                                                                                                actaTAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGACCGCGTCCAGCACGTCGTGCAGCATCGGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGa                                 <  2:106769/248‑1 (MQ=255)
                                                                                                                                                                                                                 cGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGTCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCTCCATAACCAGt                                                                   <  2:409296/216‑1 (MQ=255)
                                                                                                                                                                                                                 cGATAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGt                                                                   >  1:409296/1‑216 (MQ=255)
                                                                                                                                                                                                                   aTAAATACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATa                               >  1:206620/1‑250 (MQ=255)
                                                                                                                                                                                                                      aaTACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGACCAGCGACTCCAGACTTACCAGCCACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGACGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATgc                           >  2:455279/1‑251 (MQ=255)
                                                                                                                                                                                                                       aTACGTACCGCTTCGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATgcg                          <  1:6199/251‑1 (MQ=255)
                                                                                                                                                                                                                                   tcggcAATGTCCACCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGAGGGTATGACGGGCCAGCGACTCCAGACTTTCCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACTGCAGAGTTGGTCCGCCCCGTCGCCAATTCGCTCCAGCGCGGCAATGGTTTTTCTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCGCACGCAGGCtt               <  2:441226/246‑1 (MQ=255)
                                                                                                                                                                                                                                    cGTCAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTTACGTTTGGCGATAATGCGCACGCAGGCTTCa             <  1:224026/251‑1 (MQ=255)
                                                                                                                                                                                                                                       cAATGTCCATCCGCTCGAACGCGTCCATCACGTCGTGCATCATCTGGATGGTATGACGTCCCTGCGACTCCATACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGTCAATGGTTTTACTGATCACCATAACCAGTCTCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCTCACCCAGGCTTCatc           <  2:18389/250‑1 (MQ=255)
                                                                                                                                                                                                                                       cAATGTCCATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCGCACGCAGGCTTCatc           >  1:12337/1‑250 (MQ=255)
                                                                                                                                                                                                                                              cATCCGCTCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCGCACGCAGGCTTCATCGATCGccc   >  2:137822/1‑250 (MQ=255)
                                                                                                                                                                                                                                               aTCCGCTCGAACGCGTCCAGCACCTCGAGCAGCCTCTGGCTGGAATGACGGCCAAGAGACTCCAGACTTCCCAGCAACGGCTGCTGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCCCGTCGCCAATACGCCCCAGCTCGGCAATGGTTTTACTGATAACCATACCCAGACGCAGGTCGCTCCCCGGCGGCTGACGGTTGGCGATAATGCGCACGCCGGCCTCATAGATCGcccc  >  2:530903/1‑249 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
CATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGATAAATACGTACCGCTTCGTCAATGTCCATCCGCGCGAACGCGTCCAGCACGTCGTGCAGCATCTGGATGGTATGACGGCCCAGCGACTCCAGACTTACCAGCAACGGCTGATGCTGCTGGGAGAATTTCTCCAGCGCAGTACGGCAGATTTTGTCCGCCACGTCGCCAATACGCTCCAGCTCGGCAATGGTTTTACTGATCACCATAACCAGTCGCAGGTCGCTCGCCGTCGGCTGACGTTTGGCGATAATGCGCACGCAGGCTTCATCGATCGCCAC  >  NC_000913/3906894‑3907383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑