Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	98.6 / NA	30	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/15);  total (15/15)

GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAAAAAA  >  NC_000913/3707702‑3708193                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCacaaacaa                                                                                                                                                                                                                                                    >  1:469975/1‑250 (MQ=255)                     ggATGTGTGTTTTTTTATCCCTTTGCAGTGGGTTGGCGCTGCCTGAGTGTTGTTTTGTTTTGCCCGGTCGGGTCAACGTTATGAGGTGTGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCAGAAAAGGCCCTTCGTGACTTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAACGGGATTTCCACTAACGGTGATAATCCACAAACAAAGATTAAAAAAACGTCAAACa                                                                                                                                                                                                                               <  2:47119/250‑1 (MQ=255)                         tgttgttgTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTGTTTTGTTTTTTGCCCGGTCGTGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACAGCAGTTTAACAGTGCGGATCCTCCAGAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAATCGTCAATACAGCCAACCGGGATGTACACCAACGGTGAGAATCCACAAGCAAAGAGTAAATAAACTTCAAACAGCt                                                                                                                                                                                                                            <  2:307497/248‑1 (MQ=255)                         gtttgttGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCt                                                                                                                                                                                                                            <  2:86045/250‑1 (MQ=255)                               tgttTTAACCCGTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGTTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAACCTTCAAACAGCTATTTgc                                                                                                                                                                                                                      <  2:57028/250‑1 (MQ=255)                                      aCCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCACCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGcagcag                                                                                                                                                                                                                 >  2:234011/1‑248 (MQ=255)                                       cccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGCATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAgaga                                                                                                                                                                                                              >  2:479015/1‑250 (MQ=255)                                          tttGCAGTGGGTTGGCGGTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTAGTGACCTACAATATGTCACCAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAGGATTAAAAAAACTTCAACCAGCTATTTGCAGCAGAGAAAtt                                                                                                                                                                                                          >  2:375819/1‑251 (MQ=255)                                          tttGCAGCGGGGTGTCGCTTCCCGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAAAAGCTATTTGCAGCAGAGAAAtt                                                                                                                                                                                                          >  2:375839/1‑251 (MQ=255)                                                 ggggTTGTCGCTGCCGGACCTTTTTTGTTTTTTTCCCGTTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTTAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTa                                                                                                                                                                                                   <  2:100874/250‑1 (MQ=255)                                                          gCTGCCTGCCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGCTCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAACACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                                                                          >  2:86653/1‑251 (MQ=255)                                                          gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                                                                          <  1:234011/251‑1 (MQ=255)                                                           cTGCCTGACCTTTTTTGTTTTTCGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg                                                                                                                                                                                         >  1:417341/1‑251 (MQ=255)                                                                tGACCTATTTTGTTCATTGACCGTTCGGGTAAAAGTAATGAGGCGGGGTTGCCGTACTCATTACCATCAGTGTACCCGTACGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGCGAAAACGTCAATACAGTAAACCGGGATTTCCACCAACGGCGAGAATCCACAAACAAAGATTACAAAAACTTAAAACAGCTATCTGCAGCAGAGAACTTTGAGCAACTCCAACATGAcc                                                                                                                                                                                      >  2:195979/1‑249 (MQ=255)                                                                               tttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAat                                                                                                                                                                     >  1:400141/1‑251 (MQ=255)                                                                                          gggTCAACGTTATGAGGTGGGGATGCCGTACTCATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCCACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGCAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAAc                                                                                                                                                           >  2:155280/1‑250 (MQ=255)                                                                                          gggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACa                                                                                                                                                          <  1:479015/251‑1 (MQ=255)                                                                                           ggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                                                                                                                                         >  1:232150/1‑251 (MQ=255)                                                                                              cAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAttt                                                                                                                                                       <  1:422889/250‑1 (MQ=255)                                                                                                                  gCCGTACTAATTATCATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCTGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAATAAACTTCAAACAGCTATTGGCAGCTGAGAATGTTGTTCGGCTCCAACTTAATCAGTACTATCAGCTTAATATTTAACAGGATTTGGTggtgttgttgtatgcat                                                                                                                                   <  2:313128/250‑17 (MQ=255)                                                                                                                  gCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACGTCAAACAGCGATGTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTTATAGTCAGCAACATTTGGTGATTAAGTTTTATGCAt                                                                                                                                   <  1:253087/250‑1 (MQ=255)                                                                                                                    cGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTa                                                                                                                                        <  2:27300/243‑1 (MQ=255)                                                                                                                    cGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTa                                                                                                                                        >  1:27300/1‑243 (MQ=255)                                                                                                                                    aGTGTAACAGCGCGGATCCTCCATAAAATGCCCCTCGTGAACTACAATCTGTCAACAGAATGAGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACAAGAACAATCAGATTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAACAAATCTg                                                                                                                  >  2:394394/1‑249 (MQ=255)                                                                                                                                                                                  aTCTGTTAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGc                                                                  >  1:155663/1‑251 (MQ=255)                                                                                                                                                                                                 gtgAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGc                                                    <  1:494868/250‑1 (MQ=255)                                                                                                                                                                                                    aaaaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAgg                                                >  1:183736/1‑251 (MQ=255)                                                                                                                                                                                                                             tttACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaaa                                                                                                                          >  2:44896/1‑152 (MQ=255)                                                                                                                                                                                                                             tttACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaaa                                                                                                                          <  1:44896/152‑1 (MQ=255)                                                                                                                                                                                                                                           gagaATCCACAAACAAAGATTAAACAAACTTCAAACAGCTACTTGCAACAGAGAAATTTGTGCTACTACAACATGACCAGAACAATCATCTTAATATTTAGCAACATTTGGTGATTAAGTTTTAGGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATTCCAGGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCaaag          <  2:417341/250‑2 (MQ=255)                                                                                                                                                                                                                                             gaATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAGGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGAGATCTGAGCGATTAATACCATGAACGCTAAAACGCACTGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAATATTTAAGCAAAtata       <  2:469975/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cacaaacaAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATaaaaaa  <  1:86653/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAAAAAA  >  NC_000913/3707702‑3708193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑