Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	201.7 / NA	58	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/23);  total (35/23)

CTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATAC  >  NC_000913/3682268‑3682759                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     cTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACAGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACCCGATTAACAGCAGGATTGGGCGGACGATAAC‑CATCTGACTGTT‑AGTGGCGTGGGCGGT‑AAACA‑CCGCCGCTAtt                                                                                                                                                                                                                                                   >  2:439235/1‑251 (MQ=255)  tACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCCACCGGCAAATGGCCCACCGCAGCGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGACCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGAGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTg                                                                                                                                                                                                                                                  >  1:304559/1‑251 (MQ=255)       cGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTccgg                                                                                                                                                                                                                                             >  2:162845/1‑248 (MQ=255)         ttACCGACCAGGTTATTCCGCGCACGTGCTTCTCCCATACAGCGGCCGATACCGAGGATCAGCCCCCGACCCGCTCCCGGCAAATGGACCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGCGGACGCTATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGt                                                                                                                                                                                                                                            >  2:74107/1‑250 (MQ=255)                 cAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGAtg                                                                                                                                                                                                                                   >  1:358853/1‑251 (MQ=255)                    gttaggcagcactcgcgCTTCTGACATAAAGAGGTCGATCAAGCGGATAAGCGACCGACCCTCTCCCCGCAAATGGCACACCGAAGAGAGCGTCGCCGCCAGCACGATAAAGCCACAACCCCTTACCCCTGCCCCCCCTTTAGACGACAGCAGC‑A‑ACACGCTTCACAGCGTGATTTGGTGGCCGATATC‑CCTCTGACTGTT‑CGTGGCCTCGGCGCTCAACCA‑CC‑CCGCTATTGTCAGGTAAATCGTtgtcc                                                                                                                                                                                                                                >  2:529963/17‑249 (MQ=255)                       cccgatcccACGGGCTTCTGACATAAAGCGGTCGATCCCGAGGATCAGCCCCAGACCCGCTACCGGCAAATGGCCCCCCGCAGAGAGCGCCGCCGCCAGCACGATAAAGCCACTCCCCTTTCCCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑CCCCTATTAACAGCGTGATTTGGTGAACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCa                                                                                                                                                                                                                              <  1:408291/242‑1 (MQ=255)                            ccgcccGAGCTTCTGACTTAAGGCGTCCGATCCCGAGGACCAGCCCCAGCCCCGCTCCCGGCAAATGGCCCCCCGCAGAGAGCGTCCCCCCCAGCACGATAAAGCCACTCCCCTTCCCCCCGGCCTCCCCTTTAGAAGAAAGCAGC‑A‑TCACTTTTCACACCGTTTTTTGTTGGACGATACC‑CATCGGACTTTT‑TGTGGCCTGGGCATT‑AAACT‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAgg                                                                                                                                                                                                                        <  1:263275/246‑1 (MQ=255)                             cgcACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGCGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGt                                                                                                                                                                                                                       >  1:449961/1‑251 (MQ=255)                              gcACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGtt                                                                                                                                                                                                                      >  1:96822/1‑251 (MQ=255)                               cACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCaagctcgga                                                                                                                                                                                                                      >  2:408291/1‑242 (MQ=255)                                        cTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGt                                                                                                                                                                                                              >  1:95470/1‑249 (MQ=255)                                          gACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGcc                                                                                                                                                                                                          >  1:198393/1‑251 (MQ=255)                                          gACAAAAAGCGGTCGATACCGAGGATCAGCGCCAGCCCCGCTCCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTCCCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAg                                                                                                                                                                                                            <  1:162776/249‑1 (MQ=255)                                                        gatcccgggaaTCAGCGCCAGCCCCGCTACCGGCAAAGGGCCCCCCGCAGAGAGGGTCGCCGCCAGCACGATAAAGCCACTACCCGTTCCCCCTGCCGCCCTTTTAAAAGAAAGCAGC‑A‑ACACGATTAACGGGGTGATTGGGTGGACGATATC‑CATCTGACTTTT‑AGGGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCa                                                                                                                                                                                            <  1:187922/241‑1 (MQ=255)                                                        gATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGCGTAGCCTGTCGGGATGAcc                                                                                                                                                                                             >  1:62679/1‑250 (MQ=255)                                                          tACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGc                                                                                                                                                                                          >  1:106848/1‑251 (MQ=255)                                                           aCCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATAGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGcc                                                                                                                                                                                         >  1:440312/1‑251 (MQ=255)                                                                    cagagcCAGACCCGCTACCGGCAAAGGGACCACCGCAGAGAGCGTCGCCGCCAGCACGATAACGCCACTACCCGTTACCCCTGCCGCCCATTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTGGCCTGTCGGGCTGACCAGCCCcaccac                                                                                                                                                                                  >  2:158311/5‑249 (MQ=255)                                                                    caccaccggccccGCTACCGACAAATGCCCCACCGCAGGGAGCGTCGCCCCAAACAAGATAAACCCACTCCCCTTCCCCCCTCCCCCCCCTTTAAAAAAAAGCAGC‑A‑ACACGATTAACAGGGTGTTTTGGGGGCCGATACC‑CTTCTTTCTGTT‑AGTGCCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATTACCAGCCCCACCACCg                                                                                                                                                                                <  2:440101/241‑1 (MQ=255)                                                                         ccAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGAttt                                                                                                                                                                            >  1:185476/1‑250 (MQ=255)                                                                            gACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCAGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGACAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACgg                                                                                                                                                                        >  1:51562/1‑251 (MQ=255)                                                                                cgcacccgccAAATGCCCCCCCGCAGAGAGCGTCCCCGCCAGCACAATAAACCCCCTCCCCGTCCCCCCGCCCGCCCCTTTAGAAAAAAGCAAC‑A‑ACCCGATTAACAGCGTGATTTGGGGGACGATATC‑CATCTGACTGTT‑AGTGCCCGGGGCGAA‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGc                                                                                                                                                                    <  1:158311/242‑1 (MQ=255)                                                                                 gCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGcc                                                                                                                                                                   >  1:231165/1‑251 (MQ=255)                                                                                  cTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGCATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGTTGACAAGCCCCACCACCGATTTACGGccgccg                                                                                                                                                                  >  1:413819/1‑251 (MQ=255)                                                                                    aCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAg                                                                                                                                                                                           >  1:265061/1‑224 (MQ=255)                                                                                          aaagggCCCCCCGCAAAGAGCGCCGCCCCCACCACGAAAAGGCCCCTACCCGTCCCCCCTCCCGCCCCTTAAAAAGAAGGCA‑C‑ACACCCGATTAACAGCGGGTTTTGGGGGACGATATC‑CATCTTACTGTT‑AGGGGCTTGGGTGAT‑AACCC‑CCTCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTtctc                                                                                                                                                          <  1:95462/247‑1 (MQ=255)                                                                                          aaaTGGCAAACCGCCGCGAGCGTCGCCGCCAGCACGATCCAGCCACTAACCGTTCCCCCTGCCGCCCCCTTCGAAGAAAACAGC‑A‑ACGCGATTAACAGCGAGCTTTGGTGGACGATATC‑CATCTGACTGTT‑CGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGACCGGTCTATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATAACCAGCACCACCACCGATTTACGGCAgacacgtttcgc                                                                                                                                                          >  2:59082/1‑240 (MQ=255)                                                                                                        agagagCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGCGTTTCTCCATCTTGTCGAGc                                                                                                                                             >  1:210150/1‑250 (MQ=255)                                                                                                        agagagCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGcc                                                                                                                                            >  2:412770/1‑250 (MQ=255)                                                                                                         gagggcgccgccgcCAGCCCGATAAAGCCACTCCCCGTTCCCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGCCTGTT‑AGTGGCCTGGGGGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCAt                                                                                                                                           <  1:237831/243‑1 (MQ=255)                                                                                                              cgaccccgccAGCACGATAATGCCCTTACCCGTTCCCCCTCCCGCCCCTTTAGAAAAAAGTGGA‑A‑ACACGATAAATAGTGTGATTTGTGGGACGTTACC‑CTTCGGACTGGT‑AGGGGTCGGGGCGAA‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGAGTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATAcgcg                                                                                                                                      <  1:211397/246‑1 (MQ=255)                                                                                                                 cgccgcCAGCACGATAAAGCCACTACCCGTAACCACTGCAACCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGCTTTGGTGGACGCTATC‑AATCTGACTGTT‑AGTGGCCTAGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCATGTCGGGATGACCACCACCACCACCGATTTACGGCAGCCGCGTTTCACAAACTTGTCGAGCATACGCGGc                                                                                                                                    >  1:474726/1‑250 (MQ=255)                                                                                                                  cccgcccgcACAATAAAGCCACTCCCCTTTCCCCCTGCCGCCCCTTAAGAAGAAAGCAGC‑A‑ACACGTTTACCAGCGTGTTTTGGTGGACGATACC‑CTTCTGACTGTT‑AGTGGCCGGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAg                                                                                                                                  <  1:137839/250‑1 (MQ=255)                                                                                                                      ccagcccaataaaccccctccccGTTCCCCCTGCCCCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATGTGGTGGACGATACC‑CATCTGACTGTT‑AGGGGCCGGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGcc                                                                                                                              <  1:162845/231‑1 (MQ=255)                                                                                                                      ccAGCACGATAAGGCCCCTACCCTTTCCCCCGGCCCCCCCTTAAGAAAAAAG‑AGCCA‑CCACGATAAACAGCGGGATTTGTGGGACGATATC‑CATCTGACTGTT‑AGGGGCCGGGGCGAT‑AACCA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGcc                                                                                                                              <  1:44863/251‑1 (MQ=255)                                                                                                                                 aaGCCCCTCCCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGTTTTGGTGGACAATATC‑CACCTGACTGTT‑AGTGGCCGGGGCGAA‑AAACC‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGa                                                                                                                   <  1:86945/251‑1 (MQ=255)                                                                                                                                   gCCACTCCCCTTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑CCACGATTAACCGGGTTATTTGTTGATCGATATC‑CATCTGA‑TGTTTAGTGGCCGGGGCGAT‑AACCA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATg                                                                                                                 <  1:272131/251‑1 (MQ=255)                                                                                                                                   gCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTGGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATg                                                                                                                 >  2:211120/1‑251 (MQ=255)                                                                                                                                      cctccccGTTCCCCCGCCCGCCCCTTTAAAAAAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACAATATC‑CATCTGACTGTT‑AGTGGCCTGGGCTAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCGCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAg                                                                                                              <  1:74107/247‑1 (MQ=255)                                                                                                                                       cTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGAGATTTGGTGGACGATATC‑CATCTGAATGAT‑AGTGGACTGGGAGAT‑AAACA‑CCGCCGCTATTGTCAGGAATATCGATGTGCCATCAAGGTTAACCGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGAATACGCGGCAGCGCCGACTCGGAAGATGAAgg                                                                                                             >  1:461137/1‑250 (MQ=255)                                                                                                                                             gtccccccgcccgccCCTTTAAAAAAAAGCAAA‑A‑ACACTATAAACAGCGTGTTTTGGTGGACGATATC‑CATTTGACTTTT‑AGGGGCCGGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAg                                                                                                                    <  2:398062/228‑1 (MQ=255)                                                                                                                                             gTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAg                                                                                                                    >  1:398062/1‑238 (MQ=255)                                                                                                                                               tACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGt                                                                                                      >  1:436048/1‑250 (MQ=255)                                                                                                                                                    cTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATc                                                                                                >  1:177544/1‑251 (MQ=255)                                                                                                                                                              tttAGATGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttctt                                                                                      >  1:147538/1‑251 (MQ=255)                                                                                                                                                                tAGAAGAAAACAGC‑A‑ACACGATAAACAGTGTTATTGGGGGGCCGATACC‑TATCTGTTTTTT‑AGGGGCCTGGG‑GAA‑AAAAATCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                                     <  2:231165/250‑1 (MQ=255)                                                                                                                                                                   aagaaAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCAGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTAGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACACGGac                                                                                                                     >  1:372437/1‑214 (MQ=255)                                                                                                                                                                      aaaGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATg                                                                              >  1:44238/1‑251 (MQ=255)                                                                                                                                                                       aaGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGCTATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATAAGCAGTTCTTCACGGATgg                                                                             >  2:349005/1‑250 (MQ=255)                                                                                                                                                                         gaacc‑a‑ACGCGATTAACAGCGTGTTTGGGTGGACTAT‑TCACATCTGTTTGTT‑AGTGGCGGGGGGAAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAg                                                                           <  2:95470/247‑1 (MQ=255)                                                                                                                                                                           agc‑a‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCg                                                                         >  1:359014/1‑251 (MQ=255)                                                                                                                                                                                    cGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGACTATCGATGTGCCATCAAGGATAAACGAGTAGCCTGTCGGGATGACCAGCCCAACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTCCAATCAGCAGTTCTTCACGGATGTAGCGGATacat                                                                  >  1:541491/1‑248 (MQ=255)                                                                                                                                                                                          acggCGTGATTTGGGGTACTTTATC‑CATCTGCCTGTG‑AGGGGCCGGGGCTAA‑AAACA‑CCGCCGCTATTGTCAGGTATAGCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCATCCGAGTTTCTCCATCTTTTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAg                                                            <  1:503814/248‑1 (MQ=255)                                                                                                                                                                                               gTGATTTGGGGAACGATATC‑CATCTGTCTGTT‑GGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACt                                                       <  1:412770/251‑1 (MQ=255)                                                                                                                                                                                                ggATTTGGTGGACGATACC‑CATCTGTCTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTg                                                      <  1:276293/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggcgaaaatccccgccgCTAGTGGCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGTCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGcacca              <  2:62679/240‑1 (MQ=255)                                                                                                                                                                                                                                                   acacccgccgCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGGCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATGTGAAGATACTGAAACCAGTCTCTTTAGCGATTGAACCCAATACCAGCACCACAAACAg       <  2:498921/247‑1 (MQ=255)                                                                                                                                                                                                                                                          ccgctATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACCCGGAAGATGAAGTCCCCAGTACAATCCGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATAc  >  2:158212/1‑249 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGC‑A‑ACACGATTAACAGCGTGATTTGGTGGACGATATC‑CATCTGACTGTT‑AGTGGCCTGGGCGAT‑AAACA‑CCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATAC  >  NC_000913/3682268‑3682759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑