Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,651,284 G→A intergenic (+622/‑7) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,651,2840GA96.5% 98.6 / NA 29intergenic (+622/‑7)arsC/yhiSarsenate reductase/putative uncharacterized protein YhiS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/13);  minor base C (1/0);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTTTAAAATGAGCGCATTTTGATAGTTGTTAACGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGGAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACATATA  >  NC_000913/3651061‑3651528
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
ttttAAAATGAGCGCATTTTGATAGTTGTTAACGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTATAcccc                                                                                                                                                                                                                            <  1:142898/250‑1 (MQ=255)
            cgcATTTTGATAGTTGTTAACGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATaataaat                                                                                                                                                                                                               >  1:448736/1‑251 (MQ=255)
              cATTTTGATAGTTGTTAACGATTTCCTTTACGCTACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCCTTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATAc                                                                                                                                                                                                             >  1:309983/1‑251 (MQ=255)
                       aGTTGTTAACGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACg                                                                                                                                                                                                     <  1:86220/250‑1 (MQ=255)
                          tggtAACGATTTCCTTGACGAAACTATCACTACACTTTGTTTACACACAAAGATGACCGGGTGGAGATAAAAGGGAAAGCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATTCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTGTATGTCAATTGACTTAACCCCAGGGATAATAAATACATATCACGGCg                                                                                                                                                                                                  <  2:391481/247‑1 (MQ=255)
                              aaCGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAAGCCATTCCTGTTCTAATTATCCAATTTAAGCATCTTAGCATAAAACTAATGATGAATAAGAAATTTGATGTCAATTGACTTATCCCGAGGTATAATAAATACATATCACTGCGatat                                                                                                                                                                                              <  1:198659/250‑1 (MQ=255)
                                  aTTTCCTTTCCGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTtat                                                                                                                                                                                          >  2:529188/1‑250 (MQ=255)
                                                                        acaAATATTACCGGGAGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGac                                                                                                                                                   >  1:385035/1‑251 (MQ=255)
                                                                          aaaTATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGacac                                                                                                                                                 <  2:448736/251‑1 (MQ=255)
                                                                                  ccGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTCCATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGt                                                                                                                                         >  1:32218/1‑251 (MQ=255)
                                                                                                                     tatCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCAtt                                                                                                      >  1:148827/1‑251 (MQ=255)
                                                                                                                      atCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCAtt                                                                                                      >  1:270163/1‑250 (MQ=255)
                                                                                                                                        aTTACATTTAGTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCATACACACCGAAGTGGTCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGgag                                                                                   <  2:438968/251‑1 (MQ=255)
                                                                                                                                          tACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTataa                                                                                                                                                                                                                    >  1:17082/1‑120 (MQ=255)
                                                                                                                                          tACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTataa                                                                                                                                                                                                                    <  2:17082/120‑1 (MQ=255)
                                                                                                                                              tttATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGat                                                                             >  1:419739/1‑251 (MQ=255)
                                                                                                                                                                aaaTCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATg                                                           <  2:405464/251‑1 (MQ=255)
                                                                                                                                                                                          aTTTAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGATAAAACGCCAGACACACCGAAGTGGCCTAGCGATAATTGGGAAGAACAAAAACCCATTAATTCCACATTCTCTGGAGAAGGAAATATCTCTGATCAATATGATTTAGCGCAACACCAACTTcaaca                                  >  2:452553/1‑250 (MQ=255)
                                                                                                                                                                                            ttAAACATCTTAGCATAAAACAAATGATGAATAAGCAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACAGATAATGATAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAAt                               >  2:67790/1‑251 (MQ=255)
                                                                                                                                                                                            ttAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGCTAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCCACTTCAACAAAt                               >  2:320805/1‑251 (MQ=255)
                                                                                                                                                                                             tAAACATCTTAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCGACAAAt                               <  1:140362/250‑1 (MQ=255)
                                                                                                                                                                                                      tAGCATAAAACAAATGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCg                        <  1:529188/248‑1 (MQ=255)
                                                                                                                                                                                                                  aaTGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAaca                                              <  1:85731/214‑1 (MQ=255)
                                                                                                                                                                                                                  aaTGATGAATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAaca                                              >  2:85731/1‑214 (MQ=255)
                                                                                                                                                                                                                    tgatgaATAAGAAATTTTATGTCAAGTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGAGAATTGGGAAGAACAACAACACATTAATGCCACATTTTCTGGAGAAGGATATATCTCTGATCACTATGATTTAGCGCAACACAAACTTCAACAAATTAATGCGAGCCACACGaaaccta        >  1:276485/1‑245 (MQ=255)
                                                                                                                                                                                                                      atgaATTAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACat     >  1:147245/1‑251 (MQ=255)
                                                                                                                                                                                                                        gaATAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACatat   <  2:6605/251‑1 (MQ=255)
                                                                                                                                                                                                                         aaTAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACatat   >  1:456867/1‑250 (MQ=255)
                                                                                                                                                                                                                         aaTAAGAAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACAtata  <  2:270163/251‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
TTTTAAAATGAGCGCATTTTGATAGTTGTTAACGATTTCCTTTACGATACTATCACTACCCTTTTTTTACACACAAATATTACCGGGTGGAGATAAAAGGGAAATCAAAGGTAATTATATCAGGAAGATATAACGCATTACATTTATTGTGTGTAGAAGAAAATCCATTCCTGTTCTAATTATCCAATTTAAACATCTTAGCATAAAACAAATGATGAATAAGGAATTTTATGTCAATTGACTTTACCCCAGGTATAATAAATACATATCACGGCGATATTTATAACTGCACAACAAATACCGATAATGCTAAAACGCCAGACACACCGAAGTGGCCTTGCGATAATTGGGAAGAACAACAACCCATTAATTCCACATTCTCTGGAGAAGGATATATCTCTGATCAATATGATTTAGCGCAACACCAACTTCAACAAATTAATGCGTGCCACACGAACACTACATATA  >  NC_000913/3651061‑3651528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑