Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,638,300 G→A G220D (GGC→GAC)  pitA → metal phosphate:H(+) symporter PitA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,638,3000GA98.6% 228.2 / NA 73G220D (GGC→GAC) pitAmetal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (47/25);  total (48/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGC  >  NC_000913/3638062‑3638546
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      
ccGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAGGTATTTTCGGTTCTCGGATCGTTCCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTTATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCGGACCCCAGCGGGGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGGGGCGttgtc                                                                                                                                                                                                                                            <  2:360228/250‑4 (MQ=255)
  gTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGGATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGc                                                                                                                                                                                                                                          >  2:95659/1‑251 (MQ=255)
     aGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTTTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCCCCTGACCCCAGCGGAGCGGGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGGTATCGACGGGGCGTTTTCGcccg                                                                                                                                                                                                                                       >  2:97494/1‑248 (MQ=255)
     aGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCAACAAGAAACGCGACCGTATCCACCTGAACCCAGCGGCGCGTGAAAAGAAAAACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTCTCGACGTGGCGTTTTCGcccg                                                                                                                                                                                                                                       >  2:312123/1‑248 (MQ=255)
           ggATGCACTCAATATCACGACAGCATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGCAACGCGCCCGCATCCACCTGACCCCAGCAGAGCGGGAAAAGAAAGACGGCAAGCAAAAGCCGCCGGTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGAGGCGTTTTCGCACGGcgcg                                                                                                                                                                                                                                  >  2:56165/1‑250 (MQ=255)
                    cAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGt                                                                                                                                                                                                                         <  1:168374/250‑1 (MQ=255)
                          cccGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTCCTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGGGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGGATTGCGCTGATCCCTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACCATGGTCCGaa                                                                                                                                                                                                                    >  2:29171/1‑249 (MQ=255)
                            cGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAACCGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGc                                                                                                                                                                                                                <  2:145165/251‑1 (MQ=255)
                              aaaGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAt                                                                                                                                                                                                              >  1:132918/1‑251 (MQ=255)
                                agtattaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGACTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGATGGGCACCAAGAAACGCGGCCGTATCCACCTGACCCCCGGGGAGCGCGAAAAGAAAGAAGGCAAGAAAAAACCGCCGTTCTGAACGCGTATTGCGCTGGTCCTTTCCGCTATCGACGTGGCGGTTTCGCCCGGCGCGAACGATGGTCAGCAAGGCAttt                                                                                                                                                                                                            >  1:91400/1‑250 (MQ=255)
                                 gtattaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGGGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTCTTGCGCTGATCCTTTTCGCTATCGACGTGGCGCTTTCGCCCGGCGCGAACGATGGTCAGAAAGGCATTgg                                                                                                                                                                                                           >  1:241839/1‑251 (MQ=255)
                                  tattaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGt                                                                                                                                                                                                          >  1:109827/1‑251 (MQ=255)
                                   attaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGt                                                                                                                                                                                                          <  1:312123/250‑1 (MQ=255)
                                   attaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTc                                                                                                                                                                                                         <  1:95659/251‑1 (MQ=255)
                                   attaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTc                                                                                                                                                                                                         <  1:265835/251‑1 (MQ=255)
                                     taagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTg                                                                                                                                                                                                       >  1:370954/1‑251 (MQ=255)
                                     taaggtttttCGGTTCTCGGATCGTTCCCCCTATGGTCGGCCGGGTGTTTGCGGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGGATTGCGCTGATCCTTTCCGCTATCGACGGGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCt                                                                                                                                                                                                        <  2:221467/244‑1 (MQ=255)
                                        gtattTTCGGTGCTCTGATCGTTTCCCCTATTGGCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCCCCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGtt                                                                                                                                                                                                    <  2:394486/251‑1 (MQ=255)
                                            tttCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGCGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAACCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGGCAGAAAGGCATTGGTCTGGGTATGt                                                                                                                                                                                                >  1:319260/1‑251 (MQ=255)
                                                   tctcTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTatt                                                                                                                                                                                         >  1:496480/1‑251 (MQ=255)
                                                     tctGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTattgt                                                                                                                                                                                       >  2:270637/1‑250 (MQ=255)
                                                      ctGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAGGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTattgat                                                                                                                                                                                      >  1:404064/1‑251 (MQ=255)
                                                        gttCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTattgattg                                                                                                                                                                                    <  2:97107/249‑1 (MQ=255)
                                                        gATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGCGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTTCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTattgattg                                                                                                                                                                                    >  1:10635/1‑251 (MQ=255)
                                                        gATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTattgattg                                                                                                                                                                                    <  1:486649/251‑1 (MQ=255)
                                                           cGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGCTGGTCAGAAAGGCATTGGTCTGGTTATGTTGGGATTGATTGGCg                                                                                                                                                                                 >  1:138068/1‑251 (MQ=255)
                                                           cGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGc                                                                                                                                                                                  <  1:269635/250‑1 (MQ=255)
                                                                ccccTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACGGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTGTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTcgcg                                                                                                                                                                            <  2:370954/251‑1 (MQ=255)
                                                                  ccTATTGTCGGCCTGGTGTTTGATGGCGGTCTGATTTTCTTTCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCCAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCCATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGGCGCGcc                                                                                                                                                                          >  1:84979/1‑251 (MQ=255)
                                                                                     ttGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCCCCAAGAAAACCGCCCGTATCCACCCGACCCCCGCGGAGCGTGAAAAGAAAGACGGCCAGAAAAAACCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTCTCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTTTGTTGGTATTGTTTAGCGTCGCGCCAGCAGGCTTCgtggtctcc                                                                                                                                                       >  2:96653/1‑247 (MQ=255)
                                                                                      tGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAAc                                                                                                                                                       >  1:367231/1‑250 (MQ=255)
                                                                                                             tgcGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGCCCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGATTGCCACTGGCTACGAAATc                                                                                                                               >  2:145444/1‑251 (MQ=255)
                                                                                                                     tACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCacccgtac                                                                                                                       >  1:393499/1‑251 (MQ=255)
                                                                                                                              ggCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGCGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGc                                                                                                              >  1:437600/1‑251 (MQ=255)
                                                                                                                                cACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCa                                                                                                            <  1:333701/251‑1 (MQ=255)
                                                                                                                                 aCCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCAt                                                                                                           >  1:295533/1‑251 (MQ=255)
                                                                                                                                   cAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATc                                                                                                          >  1:332136/1‑250 (MQ=255)
                                                                                                                                    aaGAAACGCGCCCGTATCCACCTGCCCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAACAAGCCGCCGTTCTGAACGCGTATTGCGATGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGCCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCAACTGGCTACGAAATCACCCGTACCCGTGATGCAATca                                                                                                         >  2:538912/1‑250 (MQ=255)
                                                                                                                                      gAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATcaac                                                                                                       >  1:429979/1‑250 (MQ=255)
                                                                                                                                          cgcgCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGt                                                                                                  <  1:270637/251‑1 (MQ=255)
                                                                                                                                             gcCCATATCCCCCTGCCCCCAGGGGGGCGTGAAATGAACGACGTCAAGAAAAACGCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTTCCCGTGATGCCATCCACAACGTCGa                                                                                               <  2:298836/251‑1 (MQ=255)
                                                                                                                                              cccGTAGCCACCGGTCCCCAGCGGAGCGTGAAAAGAAAGACGGCAATAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTTGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCATCAACGTCGaa                                                                                              <  2:319260/251‑1 (MQ=255)
                                                                                                                                                   aTCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCtt                                                                                          >  2:208594/1‑250 (MQ=255)
                                                                                                                                                   aTCCACCTGACCCCAGCGGAGCGAGAAAAGAAAGACGGCAAGAAAAAGCAGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGACATCACCCGTACCCGTGATGCCATTAACAACGTCGAAGCtt                                                                                          >  2:89274/1‑250 (MQ=255)
                                                                                                                                                           gACCCCATCGGAGCGGGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGTTATTGATTGGCGTCGCGCCAGCAGGCTTCTTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGa                                                                                   <  2:518501/249‑1 (MQ=255)
                                                                                                                                                              ccaaGCGGAGCGTGAAAAGAAAGACGGCAAAAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGagcagc                                                                              <  2:84979/248‑1 (MQ=255)
                                                                                                                                                                    ggAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATcaacaa                                                                                                     >  1:266837/1‑222 (MQ=255)
                                                                                                                                                                    ggAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAAGCACCCGTACCCGTGATGCCATcaacaa                                                                                                     <  2:266837/222‑1 (MQ=255)
                                                                                                                                                                        gtgaaaaaGAAAGACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCgct                                                                    <  2:249034/247‑1 (MQ=255)
                                                                                                                                                                             aaagaaagACGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTc                                                                >  1:473625/1‑250 (MQ=255)
                                                                                                                                                                              aagaaagaCGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCgctgctaa                                                               >  2:357037/1‑248 (MQ=255)
                                                                                                                                                                                gaaagaCGGCAAGAAAAAGCCGCCGTTCTGAACTCGTATTACGCTGATCCATTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATAGATTGGAGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGCCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGCGCAGCATCCTGCGCTGCTCaacc                                                            >  1:347985/1‑249 (MQ=255)
                                                                                                                                                                                gaaagaCGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGGATTGATTGGCGTAGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCAACCGTACCCGCGATGCCATCAACACCGTCGACGCTTACTTTGCGCAGCATCCTGCGCTGCTCaaa                                                             >  2:368004/1‑250 (MQ=255)
                                                                                                                                                                                 aaagaCGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAAc                                                            >  1:364564/1‑250 (MQ=255)
                                                                                                                                                                                 aaagaCGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACa                                                           <  2:224379/251‑1 (MQ=255)
                                                                                                                                                                                    gaCGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGc                                                        >  1:416931/1‑251 (MQ=255)
                                                                                                                                                                                      cGGCAAGAAAAAGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCAGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCt                                                       >  1:309286/1‑250 (MQ=255)
                                                                                                                                                                                              aaaaGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCgtggct                                              >  2:189845/1‑246 (MQ=255)
                                                                                                                                                                                               aaaGCCGCCGTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTg                                                                        >  1:323250/1‑224 (MQ=255)
                                                                                                                                                                                               aaaGCCGCCGTTCTGAACGCGGATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTAGTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTg                                                                        <  2:323250/224‑1 (MQ=255)
                                                                                                                                                                                                 aGCCGCCGGTCTGTACGCGTATTGCGCTGATCCTTTCCGCTATCGACGAGGCGTTTTCGCCCGGCGCGAACGATGGCCAGAAAGGCATTGGTCTGGTTATGATGGCATTGATTGGCGTCGCGCAAGCAGGCTTCAGGATGAACATGAATGCAACTGGCTACGAAATCACCCGTACCCGAGATGCCATCAACACAGTCGAAGCTTAATTTGAGCAGCCTCCTGCGATGCTCAAGCAGTCCACCGGTGGTGac                                           >  2:6639/1‑250 (MQ=255)
                                                                                                                                                                                                     gccgTTCTGAACGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGt                                       <  2:393499/251‑1 (MQ=255)
                                                                                                                                                                                                             gaaCGCGTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTAc                                 <  1:97494/247‑1 (MQ=255)
                                                                                                                                                                                                                cgcgTATTGCGCTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGcc                            >  1:5707/1‑250 (MQ=255)
                                                                                                                                                                                                                        gcgcTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGCGCTGATCAGTTAGTACCGGCTCCGGAAGc                    >  1:76721/1‑251 (MQ=255)
                                                                                                                                                                                                                         cgcTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGAGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCt                   >  2:212554/1‑251 (MQ=255)
                                                                                                                                                                                                                         cgcTGATCCTTTCAGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCCGCAAGGCATTGGTCTGGTTATGTTGGTATTGATTGACGTCGCGCCAGCAGGCTTCGTGTTGAACATGAATGCCCCTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCTAAGCTTACTTTGAACAGCCTCCTGCGCTGCTCAAACATGCTACCTGTGCTGATACGTTAGTACCGGCTCCGGAAGCt                   >  2:213083/1‑251 (MQ=255)
                                                                                                                                                                                                                          gcTGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTg                  <  1:29171/251‑1 (MQ=255)
                                                                                                                                                                                                                            tGATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGgc                >  1:129694/1‑251 (MQ=255)
                                                                                                                                                                                                                             gATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGgcg               >  2:17282/1‑251 (MQ=255)
                                                                                                                                                                                                                             gATCCTTTCCGCTATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGgcg               >  2:105481/1‑251 (MQ=255)
                                                                                                                                                                                                                                         tATCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATACCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCCACGGAAGCTGGCGCAACGCAACCTg   >  1:293941/1‑251 (MQ=255)
                                                                                                                                                                                                                                          aTCGACGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGc  >  2:235178/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      
ACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGC  >  NC_000913/3638062‑3638546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑