| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 45.0% | 2.7 / 10.8 | 20 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base T (9/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT‑AAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTC > NC_000913/3206191‑3206676 | aCTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACa < 1:455925/251‑1 (MQ=255) gCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTGTCGCGTTTATTCACGAACTGTTAATTACATAGTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGGAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACa < 2:438488/251‑1 (MQ=255) gtgAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTTTT‑AGACAAAttaaaa > 1:324393/1‑245 (MQ=255) aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACAAAAACTGGAGTTGCCAtgatga < 1:465654/251‑1 (MQ=255) ggCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCCCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTT‑ACACAAATACAAAAACAAGTGGTGCCATGATGAggggtagaaataa > 2:64266/1‑238 (MQ=255) aGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑g < 1:352067/251‑1 (MQ=255) aaTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTACCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTC‑AGACAAATACCAAAAATGGAGTTGCCACGATGAGCGGAAGGAATAAACAACA‑GGCAGAGAATAAggtaacag > 1:76524/1‑241 (MQ=255) cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑AGAAAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAATAATAAACAACA‑GGCAGGGAATAAGTTGACAGAGATTGTCGCTAACATTTCCGCCATGagatcggaagagcacc > 1:211285/1‑234 (MQ=255) ttATTTTCGCTTTGATTCACGAACTGTTAATTTCATATTCCGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTA‑AATTTTTCC‑AGAAAAATACAAAACCTGGAGTTGCCATGATGAGCAAAATTAAAAAGCACAA‑GGCAGTAAATAAGTTGACTGAGTTTTTCGTTATCTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT‑AAAgg < 2:140503/250‑1 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑AGACAAAAACAAAAACTGGAGGTGCCATGATGAGCGGAAGTAATAAAAAAAA‑AGCAGGGGATAAGGTGTCAGAGATTTTTGCCTACTTTTCCCCCAAGA‑TTTTTTCCCCCATGCCTGATGACGGGGGGGGTAAACTAAAACaaccaaaagatggcgga > 1:32831/1‑234 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑AGACAAAAAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAACAACA‑GGCAGTGAATAAATTGACAGAGATTTTCGCTAACTTTACCGCCCTGA‑TTTTTACCCCAAAGCCTGGTGACGGGGTGGGTAAAATAAAAAAACT‑AAAGGAAGCCgcaaaggcgtcg > 1:267644/1‑240 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑AGACAAATAAAAAAAATGGGGGTGCCATGATGAGCGAAAATAATAAACAAAA‑GGCAGTGGATAAATTGGCAGAGGTTGTCGCTAACTTTAACGCCCTGA‑TTTCTACCCGAATGCCTGGTGGCGTGGGGGGTAAACTAAAAAAACT‑AAAAGATGGCGAAAAGTCGGCGATGGGGGAAAttttttac > 1:524393/1‑245 (MQ=255) gggTGGCAAAAAATATAAAACCACATTTTAAGTGGTAGTTTGTGGCGGTG‑AATTTTGCC‑AAACAAATACAAAAACGGGAGTGGCCATGAGGAGCGAAATAAATAAGCAACA‑GGCAGTGAAAAAGTTGACATAGATGTTCGTTAATTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAATACAGCT‑AAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa < 2:62676/250‑1 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTC‑ATACAAATACACAAAATGGAGTTGCCCTGCTGAGGGGAAGAAAAAAACAACA‑AGGAGAGAAAAAGATTACAGAGGTTGTTGCTAACTTTAACACCCTGAATTTTTACC‑GCATGACTGATGACGTGGGGGGTAAAATAAAACAGCTCAAAGGA‑GGCGCAACGCCGTCGATGGGGGAGATTATCTACCATACGATg > 1:469868/1‑251 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCC‑A‑ACAAATACAAAAAATGGAGTTTCCATGATGAGCGGAAATAATAAAAAACAAGGC‑GGGGATAAGTTGACAGAGAATGTCGCTAACTTTACCCCCACGA‑TTTTTACCCCCATGCCTGATGGCGGGGGGGGTAAAACAAAAAAGCT‑AAAGGATGCCGGAACGTCGTCGGTGGGGGAAATTATCTACCATAAGATGTTCGACAACATGCaaaaa > 1:221792/1‑250 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTC‑AGACAAAAACAAAAAATGGAGTTTCCATGATGAGCGGAAGTAATAAACAACA‑GGCAGGGAATAAATTGACAGAGATTGTCGCTAACttttcc > 1:185180/1‑124 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 2:185180/127‑1 (MQ=255) ggggcggg‑AATTTTTCCCAAACAAATAAAAAAACTGGAGTGGCCATGAGGAGCAAAAGTAATAAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT‑AAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc < 1:432936/243‑1 (MQ=255) ggcggcG‑AATTTTTCC‑AGACAACTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAAA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT‑AAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgc > 1:169270/1‑251 (MQ=255) aaTTTTTCC‑AGACACATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTCATACGCAACA‑GGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGA‑TTTCTACCCGAATGCATGATGACGTGGTGGATAAACTAAACCAGCT‑AAAGGATGCCGAAACGACGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc > 2:479316/1‑249 (MQ=255) | ACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCC‑AGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGA‑TTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT‑AAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTC > NC_000913/3206191‑3206676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |