Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA29.2% 47.9 / 19.0 49E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (29/5);  new base A (0/14);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 2.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAA  >  NC_000913/3177499‑3177991
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
tGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTc                                                                                                                                                                                                                                                       >  1:389995/1‑250 (MQ=255)
 ggtcccccccGGGCTTGCCAGGAAACTTAACCCCGTTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTGCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCaa                                                                                                                                                                                                                                                     <  1:50616/247‑1 (MQ=255)
     ccccccGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCCCAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑t                                                                                                                                                                                                                                                >  2:138463/1‑251 (MQ=255)
                                acCGGATTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAACAA‑TTTCCCGCCGCACCTCATGACTCAtttt                                                                                                                                                                                                                     >  2:192792/1‑250 (MQ=255)
                                       tGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                                                                                                                              >  2:222045/1‑251 (MQ=255)
                                          aacacgccgctcttcctatctcccctTCAACCGCTCCGCGACGGGCAACATCTCCCACCCTTCCCCCCTCTTCAATCCCCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CACAACCAGCACAACTTCATCACGCACTGGGACAAAGGGAAGCAAGACT‑GCGGCGTGC‑CCGCTCAAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                                                                                                                           <  2:125223/227‑1 (MQ=255)
                                                tGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACCTCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCAGTTGAATAGACg                                                                                                                                                                                                      >  1:78430/1‑250 (MQ=255)
                                                   cTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCCAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                                                                                                                   >  2:26232/1‑250 (MQ=255)
                                                      tCAGTCTCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCACGTTGAATAGACGATGACg                                                                                                                                                                                                >  1:154499/1‑250 (MQ=255)
                                                            ccGCCTCTTCAATCGCTTCGCGACGGACAACCTCTTCCACCCTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGAGCTCAAAAA‑TTTCCCGCCGCCCCTCATGACTCATGTGCCCGTTGAATAGACGATGACGAAATCt                                                                                                                                                                                          >  2:357224/1‑250 (MQ=255)
                                                              cccgctccaaccGCTTCGCGCCGGGCAACACCTCCCCCTCTTCCCCCCTCTTCAAACACCCCGGCACCCTCCTCCAGTAGCAAAGGGTTTGCGCGGGGGTCGTACGCGGCAATCCGAATCTG‑CAAAACCAGCACAACTTCATCCCGCACTGGTTCAAAGGGGAGCAGGCCT‑GCGGTGGGA‑CCGCCCTAAAAAA‑TGTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                                                                                                                       <  2:177991/240‑1 (MQ=255)
                                                               ccTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                                                                                                                           >  1:125223/1‑230 (MQ=255)
                                                                     cAATCGCTCCGCGACGGGCAACATCTTCCACACTTTCCCCCTCTTCAATCACCCCGGCAACCATCTCCAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                                                                                                                                 <  1:4799/250‑1 (MQ=255)
                                                                                 gACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaa                                                                                                                                                                      >  1:129749/1‑249 (MQ=255)
                                                                                  aCGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGc                                                                                                                                                                   >  1:248794/1‑251 (MQ=255)
                                                                                                          aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCa                                                                                                                                            >  2:79013/1‑250 (MQ=255)
                                                                                                                   aaTCATCCCGGCAACCATCTCCAGTAGACAAGGGGTTTCGCTGGTGTCGTAAGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAGGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCAGCACCTCCTGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                                                                                                                   >  2:181713/1‑250 (MQ=255)
                                                                                                                   aaTCATCCAGGCAACTATCCACAGTAGACATGGGGTTTCGCTGGTGTTGTACGCGGCAATCCGA‑TCTGTCTCAACCAGCACAACTTCATCCCGCACGGGGTAAAAGGTTAGCAAGACT‑GCGTCGTGC‑CCGCGCTAAAAAA‑TTACCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                                                                                                                                  <  2:210959/251‑1 (MQ=255)
                                                                                                                    atcatcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGa                                                                                                                                                                                                        >  2:90283/1‑180 (MQ=255)
                                                                                                                    accaccccGGCAACCATCCCCAGTAGCCAAGGGTTTTCGCGGGTGTCGTCCGCGGCAATCCGAATCTG‑CTAAACCAGCACAACTTCATCCCGCACGGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGa                                                                                                                                                                                                        <  1:90283/175‑1 (MQ=255)
                                                                                                                               acccacctcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAGATCtat                                                                                                                                                                                        <  1:139883/179‑1 (MQ=255)
                                                                                                                               aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat                                                                                                                                                                                        >  2:139883/1‑185 (MQ=255)
                                                                                                                                            aGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACggg                                                                                                        >  1:90339/1‑251 (MQ=255)
                                                                                                                                            aGCCAAGGGGTTTCGCTGGTGTCGTACGAGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCACAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATCGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAACCGCGATAAAGTGTTTCTCGTGCAATAATTTCT‑CACTAGTTTTTGCCAAATGTAAAggg                                                                                                        >  2:280914/1‑251 (MQ=255)
                                                                                                                                              ccAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGc                                                                                                       >  1:38440/1‑250 (MQ=255)
                                                                                                                                                     gTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                                                                                                                                  >  1:311582/1‑201 (MQ=255)
                                                                                                                                                        tCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTgg                                                                                            >  1:387235/1‑251 (MQ=255)
                                                                                                                                                         cgctgggttcgtcggcgGCAATCCAAATCTC‑CCCAACCAGCACAACTTCACCACGCACGGGGTCAAAGGGTAGCAAGACC‑GGGGCGTGC‑CCGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGc                                                                                           <  1:298717/237‑1 (MQ=255)
                                                                                                                                                                gtCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGc                                                                                     >  1:336076/1‑250 (MQ=255)
                                                                                                                                                                      cgcgGCAACCCGAATCTG‑CTCAATCACCACACCTCCATCCCGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTAAAAAA‑TTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTa                                                                              <  1:410955/251‑1 (MQ=255)
                                                                                                                                                                       gcgGCAATCCGACTCTG‑CTCCATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTa                                                                              >  2:176469/1‑250 (MQ=255)
                                                                                                                                                                       gcgGCAATCCGAATTTG‑CGCAATCACCACAACTCCACCCCCCACTGGGTAAAAGGGTAGCAAGCCG‑GCGGCGTGC‑CCGCCCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCAttt                                                                                                                                                                                                                      <  1:402949/115‑1 (MQ=255)
                                                                                                                                                                       gcgGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCAttt                                                                                                                                                                                                                      >  2:402949/1‑115 (MQ=255)
                                                                                                                                                                         ggCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATg                                                                           >  1:469686/1‑251 (MQ=255)
                                                                                                                                                                                       g‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGCATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAAt                                                              >  1:529938/1‑250 (MQ=255)
                                                                                                                                                                                        gcacAATCAGCACACCTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACT‑GGGGCGTGC‑CCGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTa                                                                <  1:222045/245‑1 (MQ=255)
                                                                                                                                                                                               cAGCACAACTTCATCCCGCACGGGGTCAAAGGTTAGCAAGACG‑GCGGCGTGA‑CCGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAAtt                                                      <  1:49768/251‑1 (MQ=255)
                                                                                                                                                                                                      aCTTCATCACGCACTGGGTCAAAGGGTAGCATGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACACATTTGCCCGTTGAATAGACGATGCCGAAATCTATAAAGATCTAATGCAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtgtg                                               >  2:122684/1‑251 (MQ=255)
                                                                                                                                                                                                      aCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GGGGCGGGA‑CCGCGCTAAAAAT‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTGCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtgtg                                               <  1:33173/251‑1 (MQ=255)
                                                                                                                                                                                                          caccacgcgcggggTCAAAGGGTAGCAAGACT‑GCGGCGTGC‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAAt                                            <  1:484727/239‑1 (MQ=255)
                                                                                                                                                                                                          acgcacgagcgggtacAAAGGGTAGCAAGACG‑GCGG‑ATGAACCGCG‑TCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGGCCTGGCACTAATAGTGAATTAAATGTGAAtt                                           <  2:452936/236‑1 (MQ=255)
                                                                                                                                                                                                           atcaCGCACTGAGACAAAGGGCAGCAAGACT‑GCGGCGTGA‑CAGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAACAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TAGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAAttt                                          >  2:494557/1‑251 (MQ=255)
                                                                                                                                                                                                                gCACTGGGTCAAAGGGTAGCAAGCCT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTCTAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg                                     >  2:465096/1‑251 (MQ=255)
                                                                                                                                                                                                                   cTGGGTCAACGGCTAACAAGACT‑CCGGCGTGA‑CCGAGATTACAAA‑TTCCCCGCCGAACCTAATGACTACTTAGCCAGTTGAATCGACCATGACGACATCCATAAAAATCTACTGAAAAACAGCCGCGATAAAGTGTTTCTCGTGCCATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCCTTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACg                                  >  2:534483/1‑251 (MQ=255)
                                                                                                                                                                                                                      ggTCAAGGGGTAGCAA‑ACTTGGGGAGTGA‑CCGCGCAAAAAAA‑TTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTGTTTGCCAAATGTAACGGGCAGGTTGGCTGGCTTAAGCATTGTTAATTTCCTGGCACTAATAGGGAATTAAATGTGAATTTCAGCGACGttt                               <  2:38440/251‑1 (MQ=255)
                                                                                                                                                                                                                            aGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGCGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGc                         >  2:457266/1‑251 (MQ=255)
                                                                                                                                                                                                                                       aCT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGCTGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGCTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGACTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGc                >  2:276857/1‑249 (MQ=255)
                                                                                                                                                                                                                                              gcggca‑CCGCTCTAAAAAT‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTTAtgt        <  1:138463/246‑1 (MQ=255)
                                                                                                                                                                                                                                                gTGA‑CCGCGATCAAAAT‑TTTCCCGCCGCACCTCATGACGCATGTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAAGAGTGAATTAAATGTGAATGTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATgtgt      <  1:181713/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGCGCTAAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTaaa  <  1:457266/251‑1 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
TGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTG‑CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACT‑GCGGCGTGA‑CCGCGCTCAAAAA‑TTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACA‑TCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAA  >  NC_000913/3177499‑3177991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑