| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 326,791 | 0 | C | G | 22.1% | 119.2 / 12.5 | 69 | G153R (GGC→CGC) | betA | choline dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (36/17); new base G (0/15); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAAC > NC_000913/326543‑327038 | gACGCCCACCGAGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGCGTACGAATGGCCAGACTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGCCCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCgg > 2:347215/1‑251 (MQ=255) gCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGtt > 2:248898/1‑251 (MQ=255) gCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGCGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATACTCGCTTCAAACAGCGGTTTGACGCCGGGtt > 2:45104/1‑251 (MQ=255) cccACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGtt‑t > 1:470008/1‑251 (MQ=255) aCCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCACTCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑Tggg > 2:241376/1‑250 (MQ=255) tGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGAc < 1:8877/250‑1 (MQ=255) tGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGAc < 1:5474/250‑1 (MQ=255) tGCCGGCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGGCGCGATTTGGTCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGAc < 2:28067/250‑1 (MQ=255) cGTCAAAAATGATGTGATCGGTCATAGCGTGAGTCCGAATGGTCAGGTTAGGACGCTATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTTCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTc < 2:364440/251‑1 (MQ=255) aaaaaTGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCg > 1:244735/1‑251 (MQ=255) aaaTGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGGGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCggg > 2:172056/1‑251 (MQ=255) atgatgTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGcc < 1:248898/251‑1 (MQ=255) atgatgTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGcc > 2:19958/1‑251 (MQ=255) atgatgTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTAACCGGGcc > 1:369228/1‑251 (MQ=255) atgTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGC‑GGTAGTGACGCTCACCGGGCC‑ATc > 2:33014/1‑251 (MQ=255) gtgATCGGGCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTGGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGACC‑ATCa < 1:143283/250‑1 (MQ=255) gATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCAccg > 1:464653/1‑251 (MQ=255) tAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGAt > 1:277494/1‑249 (MQ=255) aGCGTGAGTACGACTGGTCAGGTTAGGAAGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑AGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGt > 1:412197/1‑251 (MQ=255) aaTGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCACTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCAcccc > 2:230226/1‑250 (MQ=255) ggACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGAAGTGACGCTCACCGGGCC‑ATCACCGACGTGATAGTCGTTTTCACCCATATCGCGAGtctc > 1:118345/1‑251 (MQ=255) aTTTGGCCTGATCGCGATAGCCACGCGAGATGCTTGCGCGCCGGCCCTGCGGCGTGAAGGTGCGAACCATAGGACCAAAACC‑TTCCTGCTGATAACCGTTGCGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGACGTGATAGTCGTTTTCACCCATATCGCGAGTCACGGCCTTg > 2:497897/1‑251 (MQ=255) tgcccTGACCGAGATAGCCCCGCGCGGTGCGGGCGCGACGCCCCGGCGGCGTGACGGTGCAACCCATCGGACCAAACCC‑TCCCGGCTAATACCCGTTGAGACCGTCCGTGCGCGGGTACCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCGGGGTT‑TGGA‑GGTAGTGACGCTCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCg < 1:230226/247‑1 (MQ=255) gaccGAGAAAGCCACGCGCGGTGCGGGCGCGACGCCCCGGCGGCGTGCCGGTGCGATCCATCGGACCAAACCC‑TTCCTGCTGATACCCGTTGAGATCGCCCGTGCGCGGGTACCCCGCCTGCACCCCCGCTTCAATCACCGCTTAAAACAGCGGATTGACGCGGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGgtagt < 1:19958/246‑1 (MQ=255) cGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATc > 2:227645/1‑193 (MQ=255) cGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATc < 1:227645/193‑1 (MQ=255) gagaTAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGGT‑TGGA‑GGTAGGGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGggc > 2:402832/1‑250 (MQ=255) ggaTAGCCCCGCGGGGTGCGGGCGCGCCGCCCCGGCGGCGTGACGGGGCGACCCATCGGACAAAACCC‑TCCCTGCTGATACCCGTTGAGATCGCCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTAAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTGGggcag < 1:144460/250‑1 (MQ=255) ccccgcggggggcgggcgggccGGCCCTGCGGCGGGACGGGGCGATCCATCGGACCAAAACC‑TCCCTGCTGATAACCTTTGAGACCGTCCGTGGGGGGGTAGCCCGCCTGCACGCCCGCTTCAATCACCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGG‑GGTAGTGCCGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTc < 1:242247/230‑1 (MQ=255) ccACGCGCGGTGCTGGCGCGACGGCGCTGCGGCGTGACGGTCCGATCCATCGGACCAAAACC‑TTCCTGCTGCTAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCACTCATCGCTTCAAACAGCGGATTGACGCCGGGGT‑TGGA‑GGAAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGGAGTAGGGCAGGCCGTc > 2:351525/1‑251 (MQ=255) gcgGTGCTGGCGCAACGGCCATGCGGCGTGACGGTGCGATCCATCGGACCAAACCC‑TTCCTGCTGCTAACCGTTGAGCTCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGG‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCCTATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGAAGTCGAGGt > 2:296659/1‑250 (MQ=255) ggTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGc > 1:115070/1‑251 (MQ=255) cTGGCGCGACGGCCCTGCGGCGTGACGGTGCGCTCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGCGGTAACTcc > 2:321962/1‑250 (MQ=255) cgcgACGCCCCTGCGGCGGGGGGGGGCGATCCGGCG‑ACCAAAACCCTTCCGGTGGATACCCGTGGAGAGCGCCCGTGCGCGGGGAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACCCCGGTTT‑TGGA‑GGTGGTGCCGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTtc < 1:73793/251‑1 (MQ=255) aCGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACCGAGGCTTGACTCCGGGGT‑TGGA‑GGTAGTGCCGCTCCCCGCGCC‑ATCACCGCCGTGAGAGACGTTTTCCCCCATATCGCGGGACACGGCCTTGCGGTAGAAGAGCAGGCAGGCGAGGTAGCtacacatctcc > 2:210588/1‑240 (MQ=255) ggCCCTGCGGCGTGACGGTGCGATCCATCGGCCCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTcc > 1:41701/1‑248 (MQ=255) cggcgTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGtt > 1:76376/1‑251 (MQ=255) gTGACGGTGCGATCCATCGGCACAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTg > 2:72730/1‑251 (MQ=255) gACGGTGCTATCCACCGG‑CCAAAACCCTCCCGGCTGATATCCGTGGAGATCGCCCGTGCGGGGGTAGCCCGCCTGCACCCCCGCTTAAACCATCGCTTCAACAAGCGGATTGACGCCGGGTT‑TGGG‑GGTAGTGCCGCTCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTgcg < 2:380885/251‑1 (MQ=255) tGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGccc > 1:414394/1‑249 (MQ=255) cacgggcccAAACCC‑TCCCTGCTGATACCCGTGAAGACCGTCCGTGCGCGGTTACCCCGCCGGCCCCCCCGCTTAAACCACCGCTACAAACAGCGGTTTGCCGCGGGTTT‑TGGA‑GTTAGTGCCGCTCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCCTGCGCCCAGTTATCga < 1:102292/244‑1 (MQ=255) cATCGGACCAAAAAC‑TTCATGCTGATAACCGTTGAGATCGTCCGGGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATGGCGTCACACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCCCCGGGCC‑ATCAACGACGTGATAGTCGTTTTCACCCATATCGAGAGTATCGGCCTTGCGGTAGTCGGGCAGGCCGTAGAGGTAGCTCCAGTTCTCCCGACCGGGTTCTAGCGCCCAGTTATCg > 2:381820/1‑250 (MQ=255) gACCAAACCC‑TCCCTGCGGAAAACCGTTGAGATCGGCCGTGGGGGGGTAGCCCGCCTGCACGCCAGCTTCAATCATCGCTTCAAACAGCGGATTGCCGCCGGGTT‑TGGG‑GGTAGTGACGCCCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATcc < 1:212940/251‑1 (MQ=255) aaaaCC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAgcg > 1:119819/1‑251 (MQ=255) aCC‑TTCCTGCTGATAACCGTTAAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGGT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCCCCGCCGGGATAGTCGTTTTCACCCATATCGAGAGTCTCGGCCTTGAGGGAGTAGGGCAGGCAGTCGAGGTATCTCCAGCTGTCCAGACTGGGGTCTTGAGCCCCGTTAACGAGATCCATCGCAt > 2:285390/1‑251 (MQ=255) cctccttgtgaacactcGTTGATACCGTGCGTGCGCGGTTCGCCCGCCTGCTGGCGTGTTCCAATCACCGTTTCAAACGGCGGTTTGAGGGCGGGTT‑GGGATGGT‑GTGCCCCTCGCCGGCCC‑ATCACCGCCTTGAGAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGTTAGTAGGGCATGCAGTCGAGGTAGCTCCAGTGCTGCAGACCGGGTGCTTGCGCCCAGTTATCGAGATCCAGCGCAtt < 2:348954/235‑1 (MQ=255) c‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTg > 1:375259/1‑251 (MQ=255) ccggctgataccctgggagaTCGTCGGGGCGCGGGTACCCCGCCTGCACGCCCGCTTCAACCATCGCTTCAAACAGCGGATTGACGCGGGGTT‑TGGA‑GGTAGTGACGCTCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGcc < 1:296659/234‑1 (MQ=255) ggtagatatccgtgaggtgtgTCCGGGCGGGGGTACCACGCGTGCCCGCCTGCTTCAATCACGGCTTAAAACGGGGGTTTGAGGCGGGGTT‑TGGG‑GGTAGGGTGGTGCACGGGGCC‑TTCTCCGCCGTGATAGTCGTTTTCGCCCATATCGCGAGTCTCTGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTGATCGAGATCCAGCGCATTTCCACg < 2:444241/231‑1 (MQ=255) gctgATACCCGTTGAGACCGCCCTGGGGGGGGTACCCCCCCGGCCCGCCCGCTTCAACCATCGCTTCAAACAGCGGATTGCCGCCGGGTT‑GGGA‑GGTAGTGACGCCCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACgg < 1:347215/251‑1 (MQ=255) acccgttgagagctggtgTGCGCGGGCAGCCCCCCTGCTCGCCCCCGTCAAGCCGCCCTTCAACCAGCGGTTTGGGGCGGGGTT‑TGGC‑GGTTGTGACGCT‑ACCCGGCCTAGCACCGCCGTGATAGTCGTTTTCCCCCATACCGCGAGTCTCGGCCGTGCGTTAGTAGGGCTGGCAGTCGAGGTAGCTCCAGGGCTCCAGACCGGGTGTTTGCGCCTAGTTATCGAGATCCAGCGCATTGCCACGGATGt < 1:507650/232‑1 (MQ=255) gtggaggccgcccGTGGGCGGTTGGCCCGCCTGCCCCCCCGCTTCAATCATCGCTTCAAACAGCGGATGGACGC‑GGGTTGTGGA‑GGTAGTGCCGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGcaca < 2:189040/240‑1 (MQ=255) gaTCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGACGGGTT‑TGGC‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCg > 1:476243/1‑251 (MQ=255) cgcccggggcggggtcccccGCCTGCCCCCCCGCTTAAATCATCGCTCAAAACGGCGGTTTGCCGCGGGGTT‑GGGG‑GGTGGGGCCGCCCCCCGGGCC‑TTCCCCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTg < 2:414394/234‑1 (MQ=255) gcccgggcgcGGGTAGCCCGCAGGCACGCCCGCTTAAACAATCGCTACAAACAGGGGATGGAGGCGGGGTT‑GGGA‑GGAGGTGACGCTCCCGGGGCA‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGa < 1:116225/245‑1 (MQ=255) ccGTGCGCGGTTAGCCCGCCTGCACGCCCGCTTCAACCACCGCTACAAACAGCGGTTTGACGCGGGGTT‑TGGA‑GGTATTGCCGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGAt < 1:37585/250‑1 (MQ=255) ccGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCGGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGCAGCACATGCCGTTGATc > 2:451168/1‑251 (MQ=255) ggcgGGTAGCCCGCCTGCCCGCCCGCTTAAATCATCGCTGAAAACGGCGGTTTGACGCGGGGTT‑GGGA‑GGTAGGGCCGCTCACGGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGcga < 1:321962/250‑1 (MQ=255) cccGCCTCCCCGCCCGTTACAATCATCGCTTCAAACAGCGGTTGGACGCCGGGTG‑TGGA‑GGTTGGGACGCCCACCGGCCC‑ATCACCGCCGTGATAGTCGTTTTCGCCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCAcc < 1:402832/251‑1 (MQ=255) cccTGCACGCCCGCTTCAGTCATCGCGTCAAACAGGGGATTGGCGCGGGGTG‑GGGA‑GGTAGTGGCGCTCACGGGTCC‑ATCTCCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCTTTGATCAGCGACGATCCACCCAg < 1:411371/250‑1 (MQ=255) ccccccccgctaaaaccaccGCTTAAACCAGCGGATTGACGCGGGGTT‑TGGG‑GGTGTTGCCCCCCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCt < 1:135862/232‑1 (MQ=255) gtttCAATCATCGCTTCAAACAGGGGTTTGCCGCGGGGTT‑TGGG‑GGTAGTGACGCCCCCCGGCCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACcgcg < 1:33014/249‑1 (MQ=255) tCAACCAGCGGATGGAGGCCGGGTT‑TGGA‑GGTAGGGCCGCCCCCCGGGCC‑ACCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATg < 1:101552/249‑1 (MQ=255) tCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcg > 1:212121/1‑251 (MQ=255) caggggATTGCCGCGGGGTT‑GGGG‑GGTAGGGCCGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGt < 1:45104/247‑1 (MQ=255) gggggtttGACGCCGGGTT‑GGGA‑GGTGGTGCCGCCCCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt < 1:241376/245‑1 (MQ=255) gATTGACGCGGGGTT‑GGGG‑GGTAGGGCCCCACCCCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTc < 1:466378/251‑1 (MQ=255) gACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATa > 1:179074/1‑250 (MQ=255) gCCGGGAT‑TGGA‑GGTAGGGACGCTCACAGGGCC‑ATCACCGCCGTGAAAGTCGTTTTCACACATATCGCGAGTCTCGGCCTTACGGTAGTAGGGCAGGCAGTCAAGGTAGCACCAGTTCTCCAGTCCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCAACGGATGTAGCACGTGCCGTTGATCAGCGGCGATCCACCCCGACCCTTGCAGCGTCCGCACTCCATGCTGCGGTTTATCATaaca > 2:60979/1‑248 (MQ=255) | GACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACC‑TTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTT‑TGGA‑GGTAGTGACGCTCACCGGGCC‑ATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCGGCCTTGCGGTAGTAGGGCAGGCAGTCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAAC > NC_000913/326543‑327038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |