| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 24.1% | 102.2 / 12.7 | 55 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (10/31); new base T (13/0); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGC > NC_000913/119149‑119634 | aCTGATTGATTGCTATCCGGATATGGCGAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGAGCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGGTAATTGTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGACCATCGTCTGGAAGCATTCGTTCGTCGGGTGAAACATTTTtctct < 2:263431/251‑1 (MQ=255) tggttgattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTtctctcc > 1:403161/4‑250 (MQ=255) ttgCTAACCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCCTCGTCTTGAAGCGTTCTTTCGGCGGGAGGAACAATTTTTTCTCGGtcgcgc > 2:206569/1‑249 (MQ=255) aTCCGGATATCGCTAAAAACATGACGTGCCTTTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTTATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGGTAATTTTCGATCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTGCTTTCGGCTGGTGAAACATTTTTCTCTCGGGCGCACGTTa < 1:541417/250‑1 (MQ=255) cTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGGCCGTTAGG‑CCTGACCattt > 1:186745/1‑250 (MQ=255) aaaaaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCatta < 1:442048/249‑1 (MQ=255) aaaaCATTACGGGCCATTGTGATATTGCGCCGGATCGGAAAGCCGATCCCGGTCCGGCATTTTATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTGGAAGCGTTCTTTCGGCGGGTGAAACATGTTTCTCTCGGGCGCACGTCAGG‑CATGACCATTAT‑Tg < 2:37025/251‑1 (MQ=255) cATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CCTGACCATTAT‑TGCGAt > 1:123855/1‑251 (MQ=255) gcGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGc < 2:55842/250‑1 (MQ=255) gggCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGG‑CATGACCCTTAT‑TGCGAttggggg > 1:445167/1‑245 (MQ=255) gtgATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTTGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACttttt < 1:87639/251‑1 (MQ=255) atatTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATTACCATTAT‑TGCGATGGGCGTGACTTTTTTAc < 1:298855/251‑1 (MQ=255) gcgcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGtt < 1:162816/250‑1 (MQ=255) gcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTCGGGCGCACGTTTGG‑CATGCCCATTAT‑TGCGATGGGCGTGGCTTTTTTACTGtttcg > 1:386715/1‑248 (MQ=255) ggATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTCGGGCGCACGTTAGG‑CATGACCCTTAT‑TTCGTTGGGCGTGACTTTTTTACTTTT‑ACccgc > 1:521127/1‑251 (MQ=255) tCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGCTATG‑CCTGACCCTTAT‑TGCGATGGGCGTGGCTTTTTTACTGTT‑ACGCGC‑Att > 1:14496/1‑251 (MQ=255) cGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACACGC‑Att < 1:143520/250‑1 (MQ=255) aaCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGGTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAggg < 1:237973/251‑1 (MQ=255) cccGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATTACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCTCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTTCAGGGa < 2:317189/250‑1 (MQ=255) aCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGa < 2:140067/251‑1 (MQ=255) tGCATTAGATTGGACACGGTTTCGTGTGCTGGTCAGCAAGGCGACAACATGACGCTATTTCCAACCTTACTGGGGTTAATTTTCGAGCGCCTGTTTAAGT‑TTGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGCCACCTTTTTTTCTCGGGGGCACGGTACG‑CATGACCCTTTTCTG‑GATGGGCGTGTCTTTTTTACTGTT‑ACCCGC‑CTGTCAGGGAGTAtttgtcaccgt > 2:79645/1‑242 (MQ=255) tttGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGAACACATGACGCTATTTACAACCTTACTGGCGTTAATTTTCGCGCGCCTGTTTACGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCAAGTTAGG‑CCTGACCATTAT‑TGCGATGGGCGCGACTTTTTTAATGTT‑ACGCGC‑ATTGAAAGGAGGATTGTTCAACGTTccc > 1:32598/1‑251 (MQ=255) ttGATTGGGCACTGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTccc < 2:345992/250‑1 (MQ=255) gATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACg < 1:397321/251‑1 (MQ=255) gtgtGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGG‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGGAACATTTTTTTCTCCGGCGGCCGGTAGG‑CATGAACATTAT‑TGCGACTGGCGTGACTTTTTTACTGAT‑CAGCGC‑ATTGCAGGGAGTATTGTTTAACGTTCCCACGCTCATGGTGTGgcagc > 1:293634/1‑248 (MQ=255) gtgCTGGTCAGCAAGGAGACAAAATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCCCGGTAGG‑CATGTCCATTTT‑TGCGGTGGGGGTGACTTTTTTTCTTTT‑ACGCGG‑ATTTCAGGGGGGATTGTTTTAAGTTTCCACGCAACTGGTGTGgccggtt > 1:147221/1‑246 (MQ=255) tgCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGG‑CATGACCcttat‑ttc > 1:417333/1‑169 (MQ=255) tgCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGc < 2:417333/171‑1 (MQ=255) ggTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTg < 1:15485/250‑1 (MQ=255) cAGCAAGGAGCCAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTATTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGG‑CATTAACATTAT‑TTCGATGGGGGTGACTTTTTTGCTGTT‑TCGCGC‑CTTGCAGGGAGTATTGTTTAACGTTCCCCCGCTACTGGTGTGGCTGCTTGTTGGttt > 2:66396/1‑251 (MQ=255) cAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGttt < 1:390140/251‑1 (MQ=255) caacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTg < 2:46601/251‑1 (MQ=255) aacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCCCGTTAGGACTTAACC‑TTCT‑TGCGATTGGGGATACTTTTTTTTTTTT‑ACGCGC‑ATTTCAGGGAGGAATTTTTAACGTTCCCCCGCCAATGGGGTGGgtgctgtatgggttgtggttgtttgg > 2:370767/1‑231 (MQ=255) acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgc < 2:386715/251‑1 (MQ=255) caTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgcg < 2:403161/251‑1 (MQ=255) caTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGCGAAAAATTTTTTTCTTGGGCGCACGGTAAG‑CCTGACCCTTAT‑TGCgc > 1:425873/1‑151 (MQ=255) caTGACGCTATTTACAACCTGACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGa < 2:425873/152‑1 (MQ=255) aTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGGGGGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCCACTGGTGTGGCTGCTGGTTGGGTTGCTGTGTGTTGgcgc > 1:357620/1‑251 (MQ=255) gACGCTATTTACAACCTTACTGGTGTTAAGTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCa < 2:147221/250‑1 (MQ=255) cGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTTGTGTGGCGGCTGATTGGTTTGCTGTGTATTGGCGCAGGt < 1:80332/251‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTaa < 1:476518/249‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAg < 1:482738/251‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTaa > 1:473680/1‑249 (MQ=255) acccTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTGCTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTc < 1:380705/249‑1 (MQ=255) cTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGCACCTTTTTATCTCCGGGGCCCGGTAGG‑CCTGACCCTTTT‑TGCGCTGCGGGTGACTTTTTTACTGTTTAC‑CGC‑CTTTCAGGGGGTATTGTTTACCGTTCCCCCTCTACTGGTGTGGCCGCTGATTGGTTTGTTGTGTATTTGCGCTGGTGAAAATCGTCTTccttt > 2:426595/1‑247 (MQ=255) tgtTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTgg < 1:386144/223‑1 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATg < 1:483639/251‑1 (MQ=255) ttttCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACCATTTTCTCTCTGGCGCACGATAGG‑CATGACCATTAT‑TGCGATGGGCGTGGCTTTTTTACTGTT‑ACGCGC‑CTTGCAGGGAGGATTGTTCAACTTTCCCACGCTACTGGTGTGGCTGCTGGTTGGTTTTCTGTGTTTTGGCGCCGGTAAAGTTCGTCTTTATTTTCATGCTTAt > 1:395185/1‑251 (MQ=255) tCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCt < 2:158232/250‑1 (MQ=255) cGAGCGCCTGTTTA‑GTGTGGGCGAGCGCTGGCAGCTTGATCATCGTCGTGAAGCGTGCGTGCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGGTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGGT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTg < 2:357620/250‑1 (MQ=255) cgcCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACCTTTTTCTCTCGGGCGCACGGTTGG‑CATGACCATTAT‑TGCGGTGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAAAGTTCCCACGCTACTGGTGTGGCTGCTGGTTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAgc > 1:409197/1‑251 (MQ=255) cgcCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTTTCGGGCGCACCGTAGG‑CATGCCCATTAT‑TGCGCTGGGCGTGACTTTTTTTCTGTT‑AC‑CGCCATTTCAGGGGGGATTTTTCAACGTTTCCACGCTACTTGTGTGGCTGCTGATTGGTTTGCTGGGTTTTGGCGCAGGGAAAGGTCCTCTTCATTAttatttttatttggccgc > 2:235833/1‑234 (MQ=255) gTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCtt < 2:123855/251‑1 (MQ=255) gggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTTT‑TGCAATGGGGGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGGTTGCTGGGGATTGGCGCAGGGAAAGTTCGTTTTCATTATTATGCTTATCTGACAGCTGCTTAACGTAAtt > 1:397813/1‑250 (MQ=255) gTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTAATTGGt < 1:149463/155‑1 (MQ=255) tGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGGGTATTTTTTAACGTTCCCACGCTACTGGGGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGGAAAGTTCGTCTTCATTATCCTGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCg > 1:404138/1‑251 (MQ=255) tGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTCT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGc > 1:525406/1‑251 (MQ=255) | ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGT‑TGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGG‑CATGACCATTAT‑TGCGATGGGCGTGACTTTTTTACTGTT‑ACGCGC‑ATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGC > NC_000913/119149‑119634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |