Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,746,495	+T	noncoding (61/77 nt)	valV →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,746,495	1	.	T	100.0%	104.9 / NA	31	noncoding (61/77 nt)	valV	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/13);  total (18/13)

ATTATTGGGTTGTATCAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGT‑CCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATTTTGCTTTTAATGGCCCATTT  >  NC_000913/1746250‑1746744                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          attattGGGTTGTATCAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGttccaa                                                                                                                                                                                                                                                       <  1:70032/251‑6 (MQ=255)   tattGGGTTGTATCAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCTCCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAAt                                                                                                                                                                                                                                                      <  2:145513/250‑1 (MQ=255)   tattGGGTTGTATCAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAAtt                                                                                                                                                                                                                                                     >  1:280179/1‑251 (MQ=255)                cAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATcc                                                                                                                                                                                                                                        >  2:119369/1‑251 (MQ=255)                   aTGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGc                                                                                                                                                                                                                                     <  1:486872/251‑1 (MQ=255)                                             cACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGcac                                                                                                                                                                                                           >  1:179475/1‑251 (MQ=255)                                                 cTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCaccacc                                                                                                                                                                                                       >  1:102184/1‑251 (MQ=255)                                                  ttGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCt                                                                                                                                                                                                      >  1:289359/1‑251 (MQ=255)                                                         tCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGCAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACAtg                                                                                                                                                                                               >  1:305551/1‑251 (MQ=255)                                                                     aGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCggt                                                                                                                                                                                   <  1:157115/251‑1 (MQ=255)                                                                      gCCAATTTGCTTAAAAGCTCTGCAAACGGGCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGGTCGCGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCggtg                                                                                                                                                                                  >  2:127521/1‑251 (MQ=255)                                                                                 tAAAAGCCCTGCAAACGGTCGAAATAGCGCAGAAAATTACTGTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCCCCTACAACGTTGCGGTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTc                                                                                                                                                                         <  2:179475/249‑1 (MQ=255)                                                                                     aGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCGGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCg                                                                                                                                                                   <  1:270562/251‑1 (MQ=255)                                                                                      gCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGGCCCCGTTGTCCCCTACAACGGTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCGTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCgg                                                                                                                                                                  <  2:96424/251‑1 (MQ=255)                                                                                           gCAACCGGTCGAAATAGCGCAGAACATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTCATATTCGTCCCCGCTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACCTGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGcacc                                                                                                                                                                                                          >  2:437047/1‑206 (MQ=255)                                                                                           gCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTTTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGTGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTATAGcacc                                                                                                                                                                                                          <  1:437047/206‑1 (MQ=255)                                                                                                       aaTAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTAGTACGCACCAGATTTTCtt                                                                                                                                                 >  2:293415/1‑251 (MQ=255)                                                                                                                 aaaaTTACGGTTTGCCTCTTGCCACCTTCCCACGCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGGTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCATGTGAACGCACCATCGTGCGTCCGGAGCTTAGTTGGTTAGAGCACCACCTTGACAGGGTGGGGGTCGGTGGGTCGAGCCGTCTCGGACTCACCAGATGTTCTTAAGCTGGtct                                                                                                                                       <  1:185014/251‑1 (MQ=255)                                                                                                                  aaaTTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTCATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGCGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCCCTCGGACGCACCAGATTTTCTTAATCTGGtctt                                                                                                                                      >  2:351232/1‑251 (MQ=255)                                                                                                                    aTTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGtcttct                                                                                                                                    <  1:224904/251‑1 (MQ=255)                                                                                                                              gCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTccc                                                                                                                          <  1:36475/251‑1 (MQ=255)                                                                                                                                                       cGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATAc                                                                                                 >  1:362514/1‑251 (MQ=255)                                                                                                                                                          tAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGtt                                                                                              >  1:17476/1‑251 (MQ=255)                                                                                                                                                                                          gCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATc                                                              <  1:293415/251‑1 (MQ=255)                                                                                                                                                                                            gTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAg                                                            <  1:365234/251‑1 (MQ=255)                                                                                                                                                                                                aTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAgg                                                        >  1:187005/1‑251 (MQ=255)                                                                                                                                                                                                  aGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAgg                                                        >  1:225596/1‑249 (MQ=255)                                                                                                                                                                                                                 agCACCCCCTTGACATGGTGGGGGTCGTTGGTTCGCGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTAAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAAc                                       >  1:315927/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTTGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATTTTGCtttt              >  1:101900/1‑250 (MQ=255)                                                                                                                                                                                                                                            ttGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGCGTCCACTCGGCCGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATTTTGCTTTTa             >  1:221533/1‑250 (MQ=255)                                                                                                                                                                                                                                             tGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTAGCCAATACGTTAAAAGATTTaca                                                                                  <  1:415489/180‑1 (MQ=255)                                                                                                                                                                                                                                             tGGTTCGAGTTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTAGAGTCCACTCGGACGCACCAGATTTTCTTACTCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTaca                                                                                  >  2:415489/1‑180 (MQ=255)                                                                                                                                                                                                                                                      t‑CCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATTTTGCTTTTAATGGCCCAttt  <  2:102184/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          ATTATTGGGTTGTATCAGATGTAAATGCGATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGT‑CCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGGTGGTTCGAGTCCACTCGGACGCACCAGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATTTTGCTTTTAATGGCCCATTT  >  NC_000913/1746250‑1746744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑