Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,725,1760TG98.8% 249.8 / NA 81E48A (GAG→GCG) glyQglycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (39/41);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGCAGGGCTTAACGCAT  >  NC_000913/3724930‑3725421
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ccccAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGcgcgcg                                                                                                                                                                                                                                                    <  2:140025/251‑1 (MQ=255)
 cccAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGGCGGGCGGCGAGAAGGCTGAACGTAAGCAGCCGCCATCGGTTCTGGCCCCAgcgcgcgc                                                                                                                                                                                                                                                   <  1:165353/251‑1 (MQ=255)
     ggCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGAc                                                                                                                                                                                                                                               >  1:370870/1‑251 (MQ=255)
       cACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGAc                                                                                                                                                                                                                                               >  2:188140/1‑249 (MQ=255)
              cGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTg                                                                                                                                                                                                                                      <  1:188140/251‑1 (MQ=255)
               gTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGCCGTGAACAGTCAGGTCCAAGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTACGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTACACGGTTGGGGTTTTCGCCGTAGCGAACATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAtcgg                                                                                                                                                                                                                                     >  1:146017/1‑248 (MQ=255)
                tCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTggg                                                                                                                                                                                                                                    >  2:227549/1‑251 (MQ=255)
                     ttttCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTgagg                                                                                                                                                                                                                               >  2:376018/1‑250 (MQ=255)
                        tCCCAGTTATCTTCCACGAAACGGATGTCGTTAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGGCCGGCGATGGCTTAATGATCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGTCTGAACATAAGCAGCCGCGGTCGGTTCTGGCCCCTGCGCGCGCAGACAGGTCATTGGGTGAGAgg                                                                                                                                                                                                                             <  2:279247/250‑1 (MQ=255)
                          ccAGTTATCTTCCCCGAAACGGAGTTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTTAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTCCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCTCCATCAGTCCTGGCCCCAGCTCGCGCAGACAGTTCATTGTGTGTTAGGTTc                                                                                                                                                                                                                          <  2:385902/251‑1 (MQ=255)
                               tATCGTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGACCAGCACTTTCAGAGAACCGAGGTACAGCTACTGAATATTGTCCGGCGATGGCTTAATGATCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCAACGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTACTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcgc                                                                                                                                                                                                                      <  1:233689/250‑1 (MQ=255)
                                  cTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCg                                                                                                                                                                                                                  >  1:19418/1‑251 (MQ=255)
                                  cTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGATTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCg                                                                                                                                                                                                                  >  1:17896/1‑251 (MQ=255)
                                    tCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                                                                                                                                                                                   <  2:19682/248‑1 (MQ=255)
                                     ccACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGAc                                                                                                                                                                                                                <  2:330941/250‑1 (MQ=255)
                                      cACGAAACGGATGTCGTGAATAGTCGGGTCAATGACCAGCTCTTTCAGAGCACCGAGGTACAGCTCCTGCATATTGTCCGGCGATGGCATAATGACACCCTGGAACTGATAGAAGTGCTGTAAACGGTTGGGGTTTTCACCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGCACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGaatt                                                                                                                                                                                                              >  1:196192/1‑248 (MQ=255)
                                           aaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                                                                                                                         >  1:100747/1‑251 (MQ=255)
                                            aCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGCCCAGAGGTACCGCTCCTGAATATTGTACGGCGATGGCTTAATGAACAACTGGAAATGAAAGTAGAGCTGTAAACGGCTGGGGATTTCGACGAAGCGACCATCGGTCGGGCGACGAGAAGCCTGAACATAAGCCGCTGCCATCGGTTCTGGCCCCAGCGAGCGATGTCCGGTTATTGGGTGAGAGGTTCCCGCGCCGACTTCAATGt                                                                                                                                                                                                        >  1:424748/1‑251 (MQ=255)
                                               gaggTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCATCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCa                                                                                                                                                                                                     <  1:336626/248‑1 (MQ=255)
                                                       gAATAGTCGGGTCCATGCCCAGCTCTTGCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTg                                                                                                                                                                                              <  1:109082/250‑1 (MQ=255)
                                                         aTAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAAc                                                                                                                                                                                           <  1:270893/251‑1 (MQ=255)
                                                              cGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                                                                                                                      <  1:420420/251‑1 (MQ=255)
                                                              cGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTGCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTGCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                                                                                                                      <  1:197662/251‑1 (MQ=255)
                                                                ggTCCATGCGCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCTGCGATGGCGTAATGACCTCCTTGAATTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCAGCGGTCGGTCGACGAGAAGGCTGAACATAAGCAGGCGCCATCGGTGCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGGGAGAGGTTCCCGCGGCGACTTCCATGTCCAATGGTTGAACAATGGTg                                                                                                                                                                                    <  1:144977/251‑1 (MQ=255)
                                                                   ccATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTTAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTTGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGGTGAACAATGGTGCAg                                                                                                                                                                                 <  1:318772/251‑1 (MQ=255)
                                                                       gCCCAGCTCTTTCAGTGAAACTAGGTGCAGCTCCTGAATAGTGGCCGGCGAGGGCTTAATGGCCACCTGGTACTGTTAGTAGTGCTGTAAAGGGGTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGCGAATGCTGACCATATGCAGCCGCCAGCGGTTGTTGCCCCAGCGCGCGCAGACAGTTCATTGGGTGGGAGATTCCTGCGCCGAGTTCGATGTCCAATGGTTGAACAATGGTTCAGccc                                                                                                                                                                              <  2:387384/250‑1 (MQ=255)
                                                                       gCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCt                                                                                                                                                                             <  2:109910/251‑1 (MQ=255)
                                                                                 ttCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                                                                                                                    <  1:306029/250‑1 (MQ=255)
                                                                                 ttCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                                                                                                                    <  1:376018/250‑1 (MQ=255)
                                                                                  tCAGAGAACCGAGGTACAGCGCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa                                                                                                                                                                   <  2:315983/250‑1 (MQ=255)
                                                                                               gTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCt                                                                                                                                                                             >  1:287404/1‑227 (MQ=255)
                                                                                               gTACAGCTCCGGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCt                                                                                                                                                                             <  2:287404/227‑1 (MQ=255)
                                                                                                    gCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAgg                                                                                                                                                <  2:370870/251‑1 (MQ=255)
                                                                                                      tCCTGAATATAGTCCGGCGATGGCTTAATGACCACCTGGAACTGCTCGTAATGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTACAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAt                                                                                                                                              >  2:62577/1‑251 (MQ=255)
                                                                                                       ccTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTTCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATc                                                                                                                                             <  1:375666/251‑1 (MQ=255)
                                                                                                         tGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCAATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCa                                                                                                                                            >  1:218369/1‑250 (MQ=255)
                                                                                                          gAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCa                                                                                                                                            >  1:189327/1‑249 (MQ=255)
                                                                                                                 gTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTgg                                                                                                                                    <  2:336944/250‑1 (MQ=255)
                                                                                                                   ccGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGaa                                                                                                                                  <  1:257550/250‑1 (MQ=255)
                                                                                                                     ggCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGt                                                                                                                               >  1:48660/1‑251 (MQ=255)
                                                                                                                        gATGGCCTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCt                                                                                                                            >  1:117483/1‑251 (MQ=255)
                                                                                                                               tAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                                                                                                                       <  1:425329/249‑1 (MQ=255)
                                                                                                                                aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGCCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCCGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAACAAGCCCTGGAAGGTCCTGGTATCa                                                                                                                     >  2:235815/1‑250 (MQ=255)
                                                                                                                                aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaa                                                                                                                    <  1:227549/251‑1 (MQ=255)
                                                                                                                                         cTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGAGACCATCGGTCGGGCGACGAGAAGGCTGAACATCAGCAGCCCCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGAATCAAACTTTTGc                                                                                                            >  1:410521/1‑250 (MQ=255)
                                                                                                                                          tGGAACTGATAGTAGTGCTGTAAACGGGTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCat                                                                                                          >  1:22960/1‑251 (MQ=255)
                                                                                                                                                 gATAGTAGGGCTGTAAACGGTTGGGGTTTGCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCTCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAATGTCCTGGTATCAATCTTTTGCATAGTAttt                                                                                                   <  2:278028/251‑1 (MQ=255)
                                                                                                                                                   tagtagTGCTGTAAACGGTTGGGTTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCATCCGCCATCGGTTCTGGACCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGGGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCg                                                                                                 >  2:108600/1‑251 (MQ=255)
                                                                                                                                                    agtagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGTCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCATACAGGTCATTGGGTGAGAGGTTCCCGCGCCGGCTTCCATGTCCAATGGGTGAACAAGGGTGCAGCCCTGGCGAGCTCAGTAATCCTGGATGGTCAGGTTCAAGCCCTGTAAGGGCCTGGTATCAAACTTTTGCATATTATTTCGt                                                                                                <  1:21322/251‑1 (MQ=255)
                                                                                                                                                         tGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTctggctgg                                                                                           >  2:209595/1‑244 (MQ=255)
                                                                                                                                                           ctgcaaaCGGATGGGGTTTTCGCCGTAGCGCCCATCGGTCGGGCGCCGAGCAGGCTGAACATAAGCAGCCGCCATCGGATCTGGCCCCAGCGCGCGCAGACAGGACATTGGGTGAGCGGTTCCCGCGACGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTCATCCTGTAAGGTCAGGATCTAGCCCTGGAAGGTCCTGGACTCACCCTTTTGCTTTTTATTTCGTGCTGGAt                                                                                         >  2:396725/5‑251 (MQ=255)
                                                                                                                                                             gTAAACGGTTGGGGTTTGCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCACAGTAATCCTGTAAGGTCAGGATTAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGAt                                                                                         <  2:152326/249‑1 (MQ=255)
                                                                                                                                                               aaaCGGTTGGGGTGTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCGGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGTGGAGAGGTTCCCGCGCCGACGTCCATGTGCAATGGTTGAGCAAGGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGGAGTTCCTGGTATCAAGCTTATGCATATTATTTCGTGCTGGATACgt                                                                                     <  2:171459/251‑1 (MQ=255)
                                                                                                                                                                aaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                                    <  1:11835/251‑1 (MQ=255)
                                                                                                                                                                  cGGTTGGGGTTTTCGCCGTAGCGCCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGtgtg                                                                                  <  1:87370/251‑1 (MQ=255)
                                                                                                                                                                  cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCGGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGtgtg                                                                                  <  2:116215/251‑1 (MQ=255)
                                                                                                                                                                    gTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAATGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGa                                                                                <  2:70992/251‑1 (MQ=255)
                                                                                                                                                                             tacgccgaaacGACCGTCGGTCGGGCAACGAGAATGCTGAACTTAAGCACCCGACATCGGCTCTGGCCCCAGCGCACGAAGCCAGGTCATTGGGTGAGCGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCAAGTAATCCTGACAGGTCAGGATCAGACCCTGGCAGGTCCTGGTATCAACCTTTTGCATATTATTTCGTGCTGGATACGTGTGGGTTAAaagg                                                                        >  2:62201/11‑250 (MQ=255)
                                                                                                                                                                                  cGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAggg                                                                   >  1:131513/1‑250 (MQ=255)
                                                                                                                                                                                      gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGGCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGTATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGGGGATTTAAAGGAAGGGATCAg                                                              <  2:405777/251‑1 (MQ=255)
                                                                                                                                                                                      gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAGTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAg                                                              <  2:405780/251‑1 (MQ=255)
                                                                                                                                                                                       cGACCATAGGTCGAGCGAAGAGAAGCCTGCACATAAGCAGCCGCCATCGGTTCTCGCCCCAGAGCGCGCAGACAGGTCATTGGGTGCGAGGTTCCCGCGCCGCCTTCCATGTCCAATGGTTGCCCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTACAGGAAGGGATCAGt                                                             >  2:381633/1‑251 (MQ=255)
                                                                                                                                                                                        gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                                    <  2:199049/227‑1 (MQ=255)
                                                                                                                                                                                        gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                                    >  1:199049/1‑227 (MQ=255)
                                                                                                                                                                                          ccATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGCTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAACCTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGtat                                                           >  2:20070/1‑250 (MQ=255)
                                                                                                                                                                                                      cgacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatgg                                              >  1:281375/1‑251 (MQ=255)
                                                                                                                                                                                                        acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGaa                                            <  2:17896/251‑1 (MQ=255)
                                                                                                                                                                                                          gagaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATCCCCGCtggatgga                                             >  2:269818/1‑248 (MQ=255)
                                                                                                                                                                                                          gagaAGGCTGAACATAAGCAGCCACCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGCGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAgg                                          >  2:111963/1‑250 (MQ=255)
                                                                                                                                                                                                                gCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATAc                                     >  1:303467/1‑250 (MQ=255)
                                                                                                                                                                                                                    aaCATAAGCAGACGCCATCGGTGCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTGCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCGGGTATCAAACATTTGCATATTAGTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTAc                               <  1:282438/251‑1 (MQ=255)
                                                                                                                                                                                                                    aaCATAAGCAGACGCCATCGGATCTGGCCACAACGCGCGCAGACAGGTCATTGGGTGCGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGCACAATGGTGCAGCCCTGGCGCGCCCAGTAATCATGTAAGGTCAGGATCAAGCCCTGGAAGGTCATGGTATCAAACTTTTGCATATTATTTCGTGCTGGAAACGTGAGGCTTTAAAGGATGGGATCAGTATACACGCTGGATGGAAGATATACA‑GTAc                               >  2:125186/1‑251 (MQ=255)
                                                                                                                                                                                                                         aaGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATGCCCGCTGGATGGAAGATATACA‑GTACGaaa                           <  1:266150/250‑1 (MQ=255)
                                                                                                                                                                                                                         aaGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAAc                          >  1:56891/1‑251 (MQ=255)
                                                                                                                                                                                                                          agcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAGTGGTTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACGATGGTGCAGCCCTGGCGAGCCCAGTAAGCCTGTAATGTCAGGAGCAAGCCCTGGAAGGTCCGGGGATCAAACTTTGGCAAATTATTTCGTGCTGGATACGTGTGGATTAAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACg                         <  1:254127/251‑1 (MQ=255)
                                                                                                                                                                                                                             agcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACggg                       >  2:241955/1‑250 (MQ=255)
                                                                                                                                                                                                                                   cATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGCGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACgcctagggaaaacgc                >  2:110980/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cATCGGTTATGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTACGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGCTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGc                >  2:148565/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGCTATACA‑GTACGAAACGGGAAAAAGCAgg             >  2:319170/1‑251 (MQ=255)
                                                                                                                                                                                                                                         ttCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGCAGGGCt          >  1:304463/1‑251 (MQ=255)
                                                                                                                                                                                                                                          tCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGCAGGGCt          <  2:303467/250‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGGCCCCAGCGCGAGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACACTGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGAATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGAAAGGGATCAGTATACCCGCTGGATGGAAGATAT‑CACGTACGAAACGGGAAAAAGCAGGGCtt         >  2:50894/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 ccAGCGCGCGCAGACAGGTCAGTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTGCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGCAGGGCTTAACGCAt  <  2:281375/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
CCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACA‑GTACGAAACGGGAAAAAGCAGGGCTTAACGCAT  >  NC_000913/3724930‑3725421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑