Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,444,896	G→A	R112C (CGC→TGC)	rpsE ←	30S ribosomal subunit protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,444,896	0	G	A	100.0%	159.1 / NA	50	R112C (CGC→TGC)	rpsE	30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/21);  total (29/21)

AGCAGCGTTGCCTTGTGTTTCGGCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAG  >  NC_000913/3444649‑3445137                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  agcagcGTTGCCTTGTGTTTCGGCAGACGACCGATTGCACAGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGCTTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGCACGGCGCACAt                                                                                                                                                                                                                                                >  1:237815/1‑251 (MQ=255)       gTTGCCTTGTGTTTAGGCAGACGACCGATTGCACTGCGGGTTTGAGAAATATTACTAGTCTTTGCCATGGTTTATTTCCCCAGCATTTCTTCAAAGGATTTAACACGCTTGGCAACGACCATTTCTGGAGAATTAATATTTTCCAGGCCAACAATCGTTGCACGAACCACGATGATCGGGTTGGTGGTACCATAGGCTTTAGCCAAAACGTTATGAACCCCCGCGACTTCAAGAACGGCGCACATTgaaac                                                                                                                                                                                                                                          >  1:170514/1‑247 (MQ=255)                  tttCGGCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCgatg                                                                                                                                                                                                                                >  2:326540/1‑250 (MQ=255)                               ccGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTAGGAACCCCAGCGACTTCCAGAACGGCGCACATGGCACCACCGGCGATGATACCGGTACCTTc                                                                                                                                                                                                                  <  2:204380/251‑1 (MQ=255)                               ccGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCGTGGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATGTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGGTGGTGGATCCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTc                                                                                                                                                                                                                  <  1:290334/251‑1 (MQ=255)                                 gATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCgg                                                                                                                                                                                                                <  1:386333/251‑1 (MQ=255)                                    tGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGa                                                                                                                                                                                                               >  1:294603/1‑249 (MQ=255)                                                ttGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCTAGAATTTCTTCAACGGATTTACCACGCTTAGCAGCGACCATTTCTGTAGAATTCCTATTTTCCAGACCATCAATAGTTGCACGAACCACGTTGGTCGGATTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCATAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAATCCGGCTGCATg                                                                                                                                                                                                  >  1:167316/1‑250 (MQ=255)                                                 tGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGa                                                                                                                                                                                                 >  2:411484/1‑250 (MQ=255)                                                  gAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAAt                                                                                                                                                                                               <  1:399034/251‑1 (MQ=255)                                                          ttAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACATTCGGAAGCCGGCTGAATGAATAcgcg                                                                                                                                                                                          >  2:19196/1‑248 (MQ=255)                                                                gTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGACTACGCGAGAACCCgg                                                                                                                                                                                 >  2:397525/1‑250 (MQ=255)                                                                gTCTTTGCCATGGTTTATTTCCCCAGAATTTCTGCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCgt                                                                                                                                                                                 <  2:364508/251‑1 (MQ=255)                                                                  cTTTGCCCTGGTTTACTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGCACCCTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGAAACCGGTACCTTCGGACGCCGGCTGCATGAATACGCGAAAACCCgtgt                                                                                                                                                                               >  1:87403/1‑251 (MQ=255)                                                                    ttGCCATGGTTTATTTCCCCAGAAGTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGTAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTTCAGAACGGCGCACATTGCACCACCGGCGATGATACCTGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGtgtg                                                                                                                                                                              <  2:165516/250‑1 (MQ=255)                                                                     tGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGa                                                                                                                                                                             >  2:302606/1‑250 (MQ=255)                                                                       ccATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTGCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCGTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCCGCATGAATACGCGAGAACCCGTGTGAaca                                                                                                                                                                          <  2:145061/251‑1 (MQ=255)                                                                        cATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAaccc                                                                                                                                                                         >  2:289146/1‑249 (MQ=255)                                                                                aTTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAAc                                                                                                                                                                  >  1:244817/1‑250 (MQ=255)                                                                                   tCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAggg                                                                                                                                                              >  1:53992/1‑251 (MQ=255)                                                                                     cccAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGgtg                                                                                                                                                            <  2:296873/251‑1 (MQ=255)                                                                                      ccAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGgtgt                                                                                                                                                           <  1:156467/251‑1 (MQ=255)                                                                                            tttCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCACTAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGc                                                                                                                                                        >  1:143584/1‑248 (MQ=255)                                                                                                  cAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCg                                                                                                                                               <  1:82436/251‑1 (MQ=255)                                                                                                           tACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAgc                                                                                                                                      >  2:313430/1‑251 (MQ=255)                                                                                                                cGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGAc                                                                                                                                 <  1:63579/251‑1 (MQ=255)                                                                                                                        aGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCAAGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGACGCCGGCTGCATGAATACGAGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATc                                                                                                                          >  1:64200/1‑250 (MQ=255)                                                                                                                          cGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTACCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGAGTGAACACCTTTAACAAGGGGTTGCAGAGTGCCGTTATTCAGCGCGACGGTAATCat                                                                                                                        >  2:222888/1‑250 (MQ=255)                                                                                                                                     ggAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGc                                                                                                                                                 >  2:358255/1‑214 (MQ=255)                                                                                                                                     ggAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTGATGAACCCCAGCGACTTCCAGATCGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGc                                                                                                                                                 <  1:358255/214‑1 (MQ=255)                                                                                                                                      gagcaTTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGGTGATCGGGTTGGTGGAACCATAGGATTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGCACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGAGACGGGCt                                                                                                           >  2:105366/5‑251 (MQ=255)                                                                                                                                        gaATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCttt                                                                                                         >  1:23459/1‑251 (MQ=255)                                                                                                                                         aaTTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGGGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCtttt                                                                                                        >  1:290663/1‑251 (MQ=255)                                                                                                                                                                  aGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGTCTTTTTCCATCGCTTTCTGGATCGctgctg                                                                               >  2:295550/1‑251 (MQ=255)                                                                                                                                                                        aCGAACCACGTTGATCGGGTGGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATGGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGATTTTTCCATCGCTTTCTGGATCGCTGCTGGAACtt                                                                         <  2:430837/251‑1 (MQ=255)                                                                                                                                                                                    gATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTa                                                              <  2:294603/250‑1 (MQ=255)                                                                                                                                                                                                ggAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaac                                                 >  1:49613/1‑251 (MQ=255)                                                                                                                                                                                                 gAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaacc                                                <  1:119517/251‑1 (MQ=255)                                                                                                                                                                                                                gCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTAcc                                 <  1:121936/251‑1 (MQ=255)                                                                                                                                                                                                                          gaggaatcccAGCGACTGCCAGAACGGCGGACAGTGAACCACCGGCGATGATCACGGTACCGTCGGAAGCCGGATGCATGAATACGCGAGAACCCGGGTGAACAGCTTTAACAGGGTGGTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATAGTGCGACGGGCGTTTTCCATCGCTTACTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAGCCAACGCGACCGTTACCAGCGCCAAcg                       <  2:167316/244‑2 (MQ=255)                                                                                                                                                                                                                               aCCCCAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATAATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGt                  >  1:261473/1‑251 (MQ=255)                                                                                                                                                                                                                                ccccAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTc                 >  2:284373/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCTACCGTTACCATCGCCAACTtcagtcag               <  2:23459/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAGCGACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCaga              <  1:411484/251‑1 (MQ=255)                                                                                                                                                                                                                                       gACTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGCAGCCGGCAGCATGAATACGAGAGCACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGAAGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTCACCAAAACCAACGCGACCGTTACCATAGCCAACTCAAGTCAGAGCtg          >  2:17974/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgt         <  1:140159/251‑1 (MQ=255)                                                                                                                                                                                                                                         cTTCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg        >  1:58909/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttCCAGAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACCACTTTAACAGGGGGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtgtg        >  2:249041/1‑250 (MQ=255)                                                                                                                                                                                                                                               gAACGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAgg    >  1:308361/1‑249 (MQ=255)                                                                                                                                                                                                                                               aaaCGGCGCACATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAAGACGCGTGAACCCGTGTGAACACCTTGAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACTGTTGCCATCGCCAACTACAGTCAGATCTGTGAAGgag  <  1:203714/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  AGCAGCGTTGCCTTGTGTTTCGGCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAG  >  NC_000913/3444649‑3445137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑