Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	190.5 / NA	50	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/27);  total (23/27)

CAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGA  >  NC_000913/3329499‑3329963                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               aatggCCCCAAAGCTGGGTGATATGGCTTTTATACGAGTGGCATCTTATGACCCCGTTACGATGTACAGCCAGGTACGCACCCGCCCCCGTGGGTCTGCCGAAGCGAAATACGGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCTTTGGCTTTTGACGTGCAGGATGGAGCCAGCTATGCCGATGCAATTCTGAAATATGAGt                                                                                                                                                                                                                          <  2:80421/247‑1 (MQ=255)              ccTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGACGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCggg                                                                                                                                                                                                             >  1:384709/1‑251 (MQ=255)                 ggTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTCCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGt                                                                                                                                                                                                            <  2:372459/249‑1 (MQ=255)                      tatGGCTATCATACGCGTGGCAACTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGATTCTGCCGAAGCGCCATACTGCGAACTGGATGTCGTG‑CCATGCGACCTGAACCGCTTTAAGCTGACGGGGTGCATGCCACCACGTTCTGAGCCGCTCCCGTTGGCTTTTTCCGTGCAGGATGGAGCCAGCTATGCCGGTGGAATTCTGAAATATGAGTTACAACAGGCgggatccaacc                                                                                                                                                                                                     >  2:121900/1‑243 (MQ=255)                                    gcgTTGCATCTTATTTCCCCGTTACGATGTTCATCCAGGTACGCACCCTCCCCCGTTGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTCTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGTGTTATAGCAGGCGGGTATCACCTGGATCG‑GAACActg                                                                                                                                                                                      <  1:397237/251‑1 (MQ=255)                                                  taCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCG‑GGACACTGCTGCGCCAGaatc                                                                                                                                                                         >  1:199683/1‑247 (MQ=255)                                                           aCGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTCCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGAGGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAGCACCTGGAGCT‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGa                                                                                                                                                              <  1:181220/251‑1 (MQ=255)                                                                                ccccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAg                                                                                                                                         <  1:146387/250‑1 (MQ=255)                                                                                    ccccccGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGTCGACCTGAACCGCGTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTaaa                                                                                                                                     <  1:224151/250‑1 (MQ=255)                                                                                         cGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAACACAGGCGGGTATCACCCGGAGCG‑GGACACTGCTGCCCCAGACTCC‑GGTTAACCAACCTGGAACGGTAGTTGCCCGAAAACAGTc                                                                                                                                >  1:48685/1‑251 (MQ=255)                                                                                         cGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCG‑GGACAATGCTGCGCCAGAATCA‑GGTTAACGAAACTGGGACCGGAGTTGCCCGTAAACAggc                                                                                                                                >  2:65928/1‑249 (MQ=255)                                                                                           tGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCGTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGAACACCCGGAGCG‑GGACACCGCCTCGCCAGACTCA‑GGGTAACGAACCTGGGACGGAAGtttacagttaacaagcgg                                                                                                                              >  2:223543/1‑235 (MQ=255)                                                                                            ggTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCCGACTCA‑GGTTAACGAACCCGGGACGGTAGGTGCCCGGAAACAGTCGGc                                                                                                                             >  2:141532/1‑251 (MQ=255)                                                                                                  gCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCt                                                                                                                       <  1:276020/251‑1 (MQ=255)                                                                                                     gAAGCGCCATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCATGCAACAACGTTCTGCGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGACATATGAGTTAAAACAGGCGGGCGTCACCTGGCGCGCGACCAC‑GCTCCGCCAGACTCA‑GGTTTACACCCCCGGAACGGGAGTTGCCAgcaacacatcagccccccagcc                                                                                                                    >  2:273829/1‑230 (MQ=255)                                                                                                          gcaattcggCGAACTGGATGTG‑TGTCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATc                                                                                                               <  2:184850/243‑1 (MQ=255)                                                                                                                  gCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGt                                                                                                                                                                                                            >  2:396124/1‑152 (MQ=255)                                                                                                                  gCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGt                                                                                                                                                                                                            <  1:396124/152‑1 (MQ=255)                                                                                                                  gCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTaa                                                                                                        <  1:32698/250‑1 (MQ=255)                                                                                                                         ggATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GGACACTGCTGCGCCAGACTCA‑GGTTAACg                                                                                                                                                               >  2:113048/1‑188 (MQ=255)                                                                                                                         ggATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GATTAACg                                                                                                                                                               <  1:113048/188‑1 (MQ=255)                                                                                                                          gATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GGACACTGCTGCGCCAGACTCA‑GGTTAAAGAACCTGGAACGGTAGTTGCCCGTAAACAGTCGGCCCCGCTGCAAGATCTGCTTAAGATTATGCt                                                                                               >  1:2383/1‑251 (MQ=255)                                                                                                                          gATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCt                                                                                               <  1:377833/251‑1 (MQ=255)                                                                                                                          gATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGCGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGCACCTGGAAAGGGAGTTGCCCGTAAACAGTCGGCCCCGCTGCACGATCTGATTAAGATTATGCt                                                                                               >  2:405548/1‑251 (MQ=255)                                                                                                                                  g‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAg                                                                                        <  1:384905/250‑1 (MQ=255)                                                                                                                                       ggtgACCTGAACTGCTTGACGCTTAGGGGATGCCTGCCACAACGTTCTGAGTCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGACAGTTATGCCGGTGCAATTCTGAAATATGAGGTAAAACAGGCGGGGAGCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAAACTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCGGCACGATCGGCTTAAGATTATGCGGAACCAGTCGGa                                                                                   <  2:422929/248‑1 (MQ=255)                                                                                                                                          gACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCG‑GAACACTGCTGCGCCCGAATCA‑GGGTAACGAACCTGGAACGGTAGGTTCCAGTAAACAGTCGGCCCCCCTGGACGATCTGCTTTAGATTTTGCTGaaaaaagcggaaac                                                                                >  2:23151/1‑242 (MQ=255)                                                                                                                                          gACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAAAGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGGAAAAGTCGGaca                                                                                 >  1:80119/1‑250 (MQ=255)                                                                                                                                             cTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACAt                                                                             >  1:102734/1‑251 (MQ=255)                                                                                                                                             cTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGAAGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGTCAGTAAACAGTCGGCCCCGCTGCACGCTATGCTTAAGATTATGCTGAAAAAGTCGGACAACAt                                                                             >  1:379636/1‑251 (MQ=255)                                                                                                                                              ggAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTTTGCTGAAAAAGTCGGACAACATg                                                                            <  2:80119/250‑1 (MQ=255)                                                                                                                                                 cccGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATc                                                                         <  2:308650/250‑1 (MQ=255)                                                                                                                                                              gccGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGt                                                              <  2:280201/247‑1 (MQ=255)                                                                                                                                                                cGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTc                                                          <  1:36634/251‑1 (MQ=255)                                                                                                                                                                  ggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATGCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCg                                                        <  1:208858/251‑1 (MQ=255)                                                                                                                                                                           ccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTc                                                          <  1:157465/240‑1 (MQ=255)                                                                                                                                                                           ccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAATCGGACAACATGATCGCCGATACGGTTTTc                                                          >  2:157465/1‑240 (MQ=255)                                                                                                                                                                                    tCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATgcgc                                       <  1:49527/250‑1 (MQ=255)                                                                                                                                                                                       gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATgcgcg                                      <  1:358322/248‑1 (MQ=255)                                                                                                                                                                                       gAGCCGCTCCCGTTGGCTGTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCt                                    <  1:273829/250‑1 (MQ=255)                                                                                                                                                                                           cGCTCCCGTTGGCTTTTGCCGGGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGGCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAt                               <  2:10300/251‑1 (MQ=255)                                                                                                                                                                                                      gCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGcc                          <  1:284201/245‑1 (MQ=255)                                                                                                                                                                                                      gCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCCGGGGCG‑GAACACTTCTGCGCCAGACTCA‑GGTTAACGAACCCGGAACGGTAGTTGCCAGGAAACAGTCGGGCACGCTGCAAGACCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATAATCCCCGAAACGGGTTTCCGCATAGTATGCCATGCGCGCTTCAATGTGcccgttcg                    >  2:284201/1‑245 (MQ=255)                                                                                                                                                                                                       cTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGCATCACCTGGAGCG‑GAACCCTGCTGCGCCAGACTCA‑GGTTAACGAAACTGGAACGGTAGTTGCCCGTAAACAGTCGGCCCCGCTGCACGCTCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCCTGCGCGCTTCAATGTGCCTGGAAc                    >  1:236522/1‑250 (MQ=255)                                                                                                                                                                                                         tttGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGCACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATg                 >  1:403726/1‑251 (MQ=255)                                                                                                                                                                                                            gCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCG‑GAACACTGCTGCGCCAGACTCA‑GGGTAACGAACCTTGGACGGTAGTTGGCCGTAACCAGTCGGCCCCGCCGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGACAAGGCATGCGCGGTTCAATGGGCCTGGAACATGGCg              >  2:102956/1‑251 (MQ=255)                                                                                                                                                                                                            gCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGc               <  1:141532/250‑1 (MQ=255)                                                                                                                                                                                                               gTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAgg                                               <  1:141958/215‑1 (MQ=255)                                                                                                                                                                                                                   aGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAAAACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGGACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCggg       >  2:216437/1‑251 (MQ=255)                                                                                                                                                                                                                        ggAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGCGCG‑GAACCCTGCTTTGCCAGAC‑CATGGTTTACGAAACTTGTAAGGTTGCTGGCACTAAACAGTCGGGCCCGCTGAACGATCTGCTTAAGATTATGATGAAAAAGACGGACAACATGATCGACGGTACGGTTTTCCGCATGCTAGGCCAAGCGCGCTTCAATGTGCCTGGCACATGGCGGGccccggtcgg  >  1:210063/1‑243 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               CAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTG‑CCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCG‑GAACACTGCTGCGCCAGACTCA‑GGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGA  >  NC_000913/3329499‑3329963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑