Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,591	T→C	K98R (AAA→AGA)	yadN ←	putative fimbrial protein YadN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,591	0	T	C	100.0%	93.7 / NA	28	K98R (AAA→AGA)	yadN	putative fimbrial protein YadN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/17);  total (11/17)

GCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGC  >  NC_000913/156345‑156832                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gcagTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGc                                                                                                                                                                                                                                               >  1:421278/1‑251 (MQ=255)          gTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTa                                                                                                                                                                                                                                        <  2:150604/249‑1 (MQ=255)            ttGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTGTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCt                                                                                                                                                                                                                                     <  1:300591/250‑1 (MQ=255)                        ggCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGAtttg                                                                                                                                                                                                                        <  1:79426/251‑1 (MQ=255)                         gCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCCATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAACGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGAtttgc                                                                                                                                                                                                                       >  1:381057/1‑251 (MQ=255)                         gCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGAtttgc                                                                                                                                                                                                                       <  2:158142/251‑1 (MQ=255)                           aCGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACAGCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGAtttgctt                                                                                                                                                                                                                     <  2:45850/251‑1 (MQ=255)                             gAACGTAAGACGCTTTAAAATCATAAACAGCAGCTTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCAAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTACTAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGAtttgctttg                                                                                                                                                                                                                   >  1:26534/1‑251 (MQ=255)                                             aaaaTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTg                                                                                                                                                                                                    >  1:138561/1‑250 (MQ=255)                                                              aTTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagct                                                                                                                                                                                  <  2:328695/251‑1 (MQ=255)                                                                   gTCGTTGCATTCAGGGCGTTAGTATACACAACGCCAGGGTTGTTAATTTGAATCTGTTTGATAGTGGAACCAGCAAGAGTGTGTAGAGCTATATTAACTCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGCCCCTTTTCTGTTTCCAAAGAAAACAGAACCGAAGGTCATTCTTGTTTTGCGAACGGTATTCGGAGGTGCGGTGCTGCAGTCAGCGGTGACGCTGAAAGGtcgagtgttagc                                                                                                                                                                             <  2:198512/251‑12 (MQ=255)                                                                                  gCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCAtt                                                                                                                                                              >  1:3245/1‑251 (MQ=255)                                                                                       aGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCatta                                                                                                                                                         <  2:311831/251‑4 (MQ=255)                                                                                                            gttAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATGCTAGCCGTGCTACCTGGTTGCGGATTTGCTCTGCTGCAGTCAACTGTGAGGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTTATTTAGGACGCCAGCATCGGTTTCACCaa                                                                                                                                    <  2:51926/251‑1 (MQ=255)                                                                                                                    gAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGc                                                                                                                             <  2:316695/250‑1 (MQ=255)                                                                                                                                                   gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTTCTGCAGTCAACGGTGATGCTGAAAGGtttagctttagc                                                                                                                                                                             <  2:95874/171‑1 (MQ=255)                                                                                                                                                   gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagctttagc                                                                                                                                                                             >  1:95874/1‑171 (MQ=255)                                                                                                                                                            gATATCAACGCCGTCAGATGGGTTGGTAATAGACATGTCGTTGTTCAAATTCCCTTTGCTGTTACCAAAGAAAACAGAACAGAAGGTCATTCGAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGATGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTCCGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCACCCTTGTTCCCGCCGTcta                                                                                    <  2:52391/251‑3 (MQ=255)                                                                                                                                                                        gTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTcac                                                                        <  1:319983/251‑1 (MQ=255)                                                                                                                                                                          cAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAATCCCTATGCTGTTCCCCAAGAAAACAGAACCGAAGTTCATTCTAGCCGAGCTACCTAGATTCGGATTTGCTCTCCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGGACGCCCGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTcac                                                                        >  1:27908/1‑249 (MQ=255)                                                                                                                                                                                ggTTGTTAATAGACATGTCGTTGTTAAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGTATTCGGATTTGCTTTGCGGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCGGCATCGATTTCACCAACGGTGGCGGTTTGCAGCAGGATCAGATCTTCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTa                                                                <  2:421278/251‑1 (MQ=255)                                                                                                                                                                                       aaTAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATcaaca                                                          >  2:366912/1‑250 (MQ=255)                                                                                                                                                                                             cATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAc                                                   <  1:88608/251‑1 (MQ=255)                                                                                                                                                                                              aTGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAcc                                                  >  1:48604/1‑251 (MQ=255)                                                                                                                                                                                                gTCGTTGTTCAAATTCCCTTTGCTGTTTCCAAAGAAAACAGAACCGAAGGTCATTCTAGCCGTGCTCCCTGGATTCGGATTTGCTTTTCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCTGGTTTCACAGGTGTTATCAACAACCAGACCa                                                 <  1:122999/250‑1 (MQ=255)                                                                                                                                                                                                   gttgttCAAAGTCCCTTTGCTGTTACCAAATAAAACAGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACTGTGATGCTGAAAGGTTTAGTTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCATACCACCCTGGTTTCCGCCGTCAACGCGGGTTTCACAGGTGTTAGCAACACCCATACCACTg                                              <  2:418886/250‑1 (MQ=255)                                                                                                                                                                                                                                       aGAACCGAAGGTCATTCTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGCTCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGc           >  2:410331/1‑249 (MQ=255)                                                                                                                                                                                                                                               aGGTCATTCTAGCCGTGCTACCTGGATTCGGCTTTGCTTAGCTGCAGTCAACGGTGATGCTGAAACGCTTAGCTTTAGCGCCAACGGAGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCACCAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGc  >  1:210937/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGC  >  NC_000913/156345‑156832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑