Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,044,642 T→C intergenic (+18/‑296) asnT → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,6420TC91.7% 71.2 / ‑0.5 25intergenic (+18/‑296)asnT/yeeJtRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (11/11);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

ATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCAT  >  NC_000913/2044402‑2044861
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                           
aTCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAgcgc                                                                                                                                                                                                                    >  2:190668/1‑250 (MQ=255)
  cACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCATATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGGATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAGTCTAAAAATTCGCTCTTTTAGCGCAAt                                                                                                                                                                                                                 <  2:143444/251‑1 (MQ=255)
  cACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAAt                                                                                                                                                                                                                 <  1:181801/251‑1 (MQ=255)
         aTGATACAGACGCGTTTTCGGCGACGAATGTGCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTg                                                                                                                                                                                                          <  2:166417/251‑1 (MQ=255)
          tGATACAGACTCGTTATCGGCGCCGAATGTTCATTAATTCACAACTTTGCCGATTAATTAACCAATTGAACTGACTTATGACATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAACAATTCGCTCTTTTAGCGCAATGTCACTg                                                                                                                                                                                                          >  2:60277/1‑250 (MQ=37)
                       ttttCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATACGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAAc                                                                                                                                                                                             >  1:279826/1‑250 (MQ=255)
                              cgacgaATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTACAATGACTTATGAAATTTAGAGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACAAGATTCCTCTGTAGTTCAGTCGGTAGAACGGCAGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGCGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTCGTTGAACATTGtttt                                                                                                                                                                                     >  1:239026/1‑251 (MQ=37)
                                    aTGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGGCACTGACCTTAGTTGAGCATTGTTTTTTAACg                                                                                                                                                                               <  1:161779/251‑1 (MQ=255)
                                                 aacaacTTTGCAGATTAATTAAACAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtttt                                                                                                                                                                  >  2:279189/1‑251 (MQ=255)
                                                          gCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCtt                                                                                                                                                         <  1:410490/251‑1 (MQ=255)
                                                                taattaaCCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcgc                                                                                                                                                   >  2:137137/1‑251 (MQ=255)
                                                                   ttaaCCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                                                                                                                                                    <  2:169578/215‑1 (MQ=255)
                                                                   ttaaCCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                                                                                                                                                    >  1:169578/1‑215 (MQ=255)
                                                                          aTTGAAATGACTTCTGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTAGCCAAGTTCACACGATTCCTCTGTAGTTCGGTAGGTAGAACGGCGGACTGTTAATCCGAATGTCACTGGTTCGAGTCAAATCAGAGGAGCAAAATTCTAACACTTCGCTCTTTTAGGGACATGTCACTGACCTTAGTTGAAACTTGTTTTTTAACGGATAGCGGGtttttcaccactttcgcgccctcgccatt                                                                                                                                         >  2:266894/1‑227 (MQ=255)
                                                                              aaaTGAATTATGAAATTTAGTGCTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAATTCAGTCGGTAGAACGGCGGACTATTAATCCGTATGTCCCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTACAAATTCGCTATTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATCGCGGGTTTTTAACCTATTAAGCTCCCTCGACCTTTATg                                                                                                                                     >  2:128399/1‑251 (MQ=37)
                                                                                  gACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTGTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTGTATGGtt                                                                                                                                  <  2:421382/250‑1 (MQ=255)
                                                                                                             aaGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc                                                                                                      >  2:287379/1‑251 (MQ=255)
                                                                                                                  aCCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAttt                                                                                                 >  2:140698/1‑251 (MQ=255)
                                                                                                                                  cGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaa                                                                                  <  2:93531/250‑1 (MQ=255)
                                                                                                                                                               cagacGGTAGAACGGCGGACTGTTACTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAACTTCTAAAAATTCGCTCTTTTAGCGCCATGTCACTGCCCTTAGTTGAACATTGttt                                                                                                                                                                                      >  1:358465/5‑121 (MQ=21)
                                                                                                                                                                         aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcaca                                          <  1:279189/251‑1 (MQ=255)
                                                                                                                                                                               ggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCaaa                                    <  1:190668/251‑1 (MQ=255)
                                                                                                                                                                                       aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTa                            <  1:140698/251‑1 (MQ=255)
                                                                                                                                                                                              aggTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGTCGTTTTGCTATGAACGCCTTCACCATTTTCCCCTCGATTATAAAACTAGAGTTATTCAGTAGTCTCCCCTCGTGCAACGCACACCCAAAACTGCCGAACGaaaa                     <  2:239026/249‑1 (MQ=255)
                                                                                                                                                                                                                  gTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCat  >  1:117932/1‑250 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                           
ATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCAT  >  NC_000913/2044402‑2044861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑