Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,433	C→A	A319D (GCC→GAC)	msrP →	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,433	0	C	A	95.6%	135.1 / NA	46	A319D (GCC→GAC)	msrP	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (17/26);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCT  >  NC_000913/2040186‑2040671                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTGTTACGCCAACGTTAATCCGTATGGTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCa                                                                                                                                                                                                                                             <  1:407868/251‑1 (MQ=255)    cGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACACCCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGt                                                                                                                                                                                                                                          >  1:269698/1‑251 (MQ=255)                           tGGCTTTAAAGGGATTAATTCGATAGTCCGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTAACGCACGATTTATTGGTACAGGCGGCCTCCTCGATGAACCGCGCCAGCCAACGCTAATGTTTAATGGTTACGCCGACCAGCAGGCATGGCTGTATCGAGGCCTgg                                                                                                                                                                                                                   >  2:284107/1‑251 (MQ=255)                            ggCTTTAAAGGGATTAAATCGATAGTCAGTAGTAAGCGGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGAGCGCCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGTCATCCGCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTTTCGGGGCCTGGa                                                                                                                                                                                                                  <  2:423202/251‑1 (MQ=255)                                  aaaGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCg                                                                                                                                                                                                            <  2:292245/251‑1 (MQ=255)                                       gATTAAATCGATAGTAAGTATTAAGCTGAACAGCGAGCGTCCGCCAACCACCTGGAATATGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGAATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTACCGCCGCACCGAGGGCATCGCGGTATCGTGGCCTGGATTTGCggg                                                                                                                                                                                                          >  2:337548/1‑248 (MQ=255)                                                           ttAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGAc                                                                                                                                                                                    >  1:357434/1‑250 (MQ=255)                                                              aGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCa                                                                                                                                                                                <  1:227513/251‑1 (MQ=255)                                                                 tGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCaaa                                                                                                                                                                              >  1:8268/1‑250 (MQ=255)                                                                     ccGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAgg                                                                                                                                                                         <  2:157807/251‑1 (MQ=255)                                                                        cgAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGAGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTg                                                                                                                                                                       >  1:150970/1‑250 (MQ=255)                                                                         gAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTCATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGCATTTCTAAATGCGTCTGCCAGCAAAACAGGTGAc                                                                                                                                                                     >  2:415032/1‑251 (MQ=255)                                                                                  ccaaccaaCTGAAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGa                                                                                                                                                             >  1:95761/1‑250 (MQ=255)                                                                                            ggAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCt                                                                                                                                                   <  2:340196/250‑1 (MQ=255)                                                                                             gAATCTGGCAGCGCCTGCCGCATCCGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTCCCGACCGATTTATTGGTTCAGGCGGCATCCTCGATGTCCAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGTCCAGGTGGCCTCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGCCATCAAAACAGGTGACATGGCTGAAAGTTTGCCTGc                                                                                                                                                 >  2:373731/1‑251 (MQ=255)                                                                                              aaTCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAGCGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTACCTGCa                                                                                                                                                <  1:112548/251‑1 (MQ=255)                                                                                                       gcgcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCgg                                                                                                                                       <  1:343765/251‑1 (MQ=255)                                                                                                         gcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGAt                                                                                                                                     <  1:369771/251‑1 (MQ=255)                                                                                                           cTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGaatg                                                                                                                                   >  1:81817/1‑248 (MQ=255)                                                                                                            tGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGAttgt                                                                                                                                  >  2:136028/1‑251 (MQ=255)                                                                                                             gACGAATACGGTTTTTACGCCATCGTTAATGCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGTTGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTAGCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGAttgtt                                                                                                                                 <  1:284107/251‑1 (MQ=255)                                                                                                             gACGAATACGGTTTTTACGCCAACGTTAATCCGTATTTTGATCACCCGCGCTGGTCACAGGCGACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGGCAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTTCATCTTGCCGGAttgtt                                                                                                                                 <  2:347707/251‑1 (MQ=255)                                                                                                               cGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGc                                                                                                                               <  1:291512/251‑1 (MQ=255)                                                                                                                 aaTACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCg                                                                                                                             <  1:373055/251‑1 (MQ=255)                                                                                                                             aCGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTctggctg                                                                                                                 >  1:195879/1‑251 (MQ=255)                                                                                                                                           ccggaTGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGGTACGCCGACCAGGTGTCATCGCTGTAGCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCAGCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGatca                                                                                                    <  2:138629/246‑1 (MQ=255)                                                                                                                                               aTGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGatcaatca                                                                                                <  1:95266/250‑1 (MQ=255)                                                                                                                                                tGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTAAGGCGGCCTCCTCGATGTACAGCGCCAGCCCACGCTACTGTATAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATATGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGAGACATGGCTGAAAGTTTGCCTGCATCTTTCCGGATTGTTGCCGTTTCTCGGGCTGGTCTGGGCGatcactcccg                                                                                              >  2:237428/1‑245 (MQ=255)                                                                                                                                                                 ggTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGGGTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATGGTTGCCGGTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGTCTGGGTGCCGa                                                                              <  2:179099/250‑1 (MQ=255)                                                                                                                                                                      caGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGGCTGACAGCAAAACAGGTGACATGGCTGAAAGTGTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGAGCCGGt                                                                        <  2:239259/251‑1 (MQ=255)                                                                                                                                                                         gCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGt                                                                                                                                                                        <  2:124024/152‑1 (MQ=255)                                                                                                                                                                         gCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGGGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGt                                                                                                                                                                        >  1:124024/1‑152 (MQ=255)                                                                                                                                                                                aaCGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGGTTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATAtt                                                              >  1:68392/1‑251 (MQ=255)                                                                                                                                                                                  cGATTTATTGGATCAGGCGCCATCCTCGCTGTACAGCGCCAGCCAACGCTACTGTTTAATGGATACGCCGACCAGGTGGCATCGCTGTATCGCGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGATGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTc                                                             >  2:46378/1‑250 (MQ=255)                                                                                                                                                                                           ggTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGGTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCTGGAGAATGTCTAAATGCGTCTGACAGCAAAACAGGTGACATTGCTGAAAGTTGGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCTCGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTAc                                                   <  1:301484/251‑1 (MQ=255)                                                                                                                                                                                            gTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAAGCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACt                                                  <  1:242127/251‑1 (MQ=255)                                                                                                                                                                                                   cggcATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGCGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCgc                                            >  2:22602/1‑250 (MQ=255)                                                                                                                                                                                                         ccTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTTCGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCgcactgcac                                     <  2:298298/251‑1 (MQ=255)                                                                                                                                                                                                                  aCAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAAttt                             <  2:397570/250‑1 (MQ=255)                                                                                                                                                                                                                              aaCGCTACGGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTAGCGTGGCCTGGAGTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCGGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCTATCAATCACGGTGGACTGGGTGCCGATCCGTTGAAAGATATTCATCATTTTACTGGTCCCACTACACTGAAATTTTTGCTGGCGACCt                <  2:269698/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGt             >  1:425337/1‑251 (MQ=255)                                                                                                                                                                                                                                  cTACTGTTTAATGGTTACGCCGACCAGGTGGAATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGtt            >  1:397401/1‑251 (MQ=255)                                                                                                                                                                                                                                          tAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCAcc     <  1:98965/250‑1 (MQ=255)                                                                                                                                                                                                                                            aTGGGTACGCCTACCAGTTGGCTTCGCTGTATCGTGGCCTGGAGTTGCGGGAGAATTTCTAAAGGCGTCTGACAGCAAAACAGGTGACAGGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTGTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCTATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAAGCACCCct  <  2:147689/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGaa                                 >  2:166415/1‑215 (MQ=255)                                                                                                                                                                                                                                                 tACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGaa                                 <  1:166415/215‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCT  >  NC_000913/2040186‑2040671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑