| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,025,277 | C→A | noncoding (37/91 nt) | dsrA ← | small regulatory RNA DsrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,025,277 | 0 | C | A | 97.2% | 128.2 / ‑3.6 | 36 | noncoding (37/91 nt) | dsrA | small regulatory RNA DsrA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (17/18); minor base G (0/1); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01 | |||||||||||
GTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATT > NC_000913/2025034‑2025522 | gtcgatgtcgatGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTaaaaa < 1:7241/251‑1 (MQ=255) cgatgtcgatgCCCTGCTGGCGGCGATCAATGCAATTAGCGAAAGAGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGAACCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAAt > 1:243196/1‑251 (MQ=255) cgatgtcgatgCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAAt < 1:229956/251‑1 (MQ=255) atgtcgatgCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTc > 1:385268/1‑251 (MQ=255) gctgGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGa > 1:334529/1‑250 (MQ=255) aTCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGAtgtg < 1:183189/251‑1 (MQ=255) gAAATTAGCGAAAGCTAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACTTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGACTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAAGTCGTTGCACCAGGAAATCTGATGTGTtcat < 2:319276/250‑1 (MQ=255) aaaTTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcatca < 1:349561/251‑1 (MQ=255) agcgaaagcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCtt < 1:211173/250‑1 (MQ=255) agcgaaagcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTa < 2:259923/251‑1 (MQ=255) gaaagcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATcc > 1:259409/1‑251 (MQ=255) aagcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTAATCACCTTATCCGc > 2:102299/1‑251 (MQ=255) agcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACTCCAGGAAATCTTTTGTGtttatagctttatgcgtt < 1:364079/251‑17 (MQ=255) agcgaaGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCa < 2:191199/251‑1 (MQ=255) ccAGAATGATTCTGAACACGTTAGCGTCGATGGCCGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACa > 1:121228/1‑250 (MQ=255) cAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAg < 2:223099/251‑1 (MQ=255) gTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatga < 2:48028/251‑1 (MQ=255) cGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGAAAAGAAAATATTCGCCATATGAATGATTa > 2:190057/1‑250 (MQ=255) gACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCt > 1:219419/1‑250 (MQ=255) cGTGAATATCATACATGGCGTGAATTGGCGGATGACTTAGCACTGGATATTCCTGACTTCAGAGTCTCTGAAGTGAATCGTTGAATGCCCCATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAACACATTCGATACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCCCTGACACGCAAATATTAGCCATATGAATGATTAACGCTACt > 2:413340/1‑251 (MQ=255) cATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATa < 2:334529/250‑1 (MQ=255) gTGAATTGGCGGAGGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAGTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGAct < 2:245229/251‑2 (MQ=255) gCCTTCGAACTGGATAGTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTTAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTCCTTATGAATAGAAATGTGTCGCACGTCACCCCtt < 2:109783/250‑1 (MQ=255) gAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGATCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTGCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATGCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTTACTCACTTCACCCCTTGGTGGct < 2:162599/251‑1 (MQ=255) aCTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGAt < 2:331493/251‑1 (MQ=255) cGTTGAATGCACAATAAAAAAATCCCGACCCTGGGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTg > 2:90906/1‑251 (MQ=255) tGAATGCACAATAAAAAAATCCAGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGAt > 1:78299/1‑250 (MQ=255) aTGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAAtt < 2:193132/251‑1 (MQ=255) aaaaaaaTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGAAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGAAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGtt > 1:339107/1‑251 (MQ=255) aaaaaTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAAGTTGTGCGGtttt < 2:23979/251‑1 (MQ=255) aTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGACGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGTGATCGCTCCCGAAAAAATCGTCTTTGATAATTTGTGCGGTTTTg > 2:81546/1‑248 (MQ=255) aaaCTTGATTAAGCAGGCAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTAATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAACTATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGCCGTCAGCGGGATCGCTCACGAAAAAATCATCTTTGATAATTTGTGAGGTTTTGAGAATCATCATGGTAAGAGGAGTACTgcg > 2:17654/1‑251 (MQ=255) tGCTTAAGCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGAc < 1:53338/250‑1 (MQ=255) gCAAGAAGCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGtttt > 1:67402/1‑251 (MQ=255) agaagCAATTAAAAAATTCGTTACACCAGGCAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGATTAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTttca > 2:343625/1‑250 (MQ=255) aagCAATTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGAAAGAGGAGTACTGCGCGACTATGTTttcaatt > 2:116532/1‑251 (MQ=255) | GTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAATAGAAATGTGACGCACGTCACCCCTTGGTGGCTCTGGCGTCAGCGGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATT > NC_000913/2025034‑2025522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |