Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	58.3 / NA	20	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

CTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTG  >  NC_000913/155826‑156304                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             cTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGac                                                                                                                                                                                                                                       >  1:158142/1‑250 (MQ=255)         ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTGGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAGTCCCTGTATTTGCCAGACGTGCGTTGACACttt                                                                                                                                                                                                                                  <  2:273280/247‑1 (MQ=255)                            gTACGTTAAACCAGAACACGCTCTCTCTTTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGGTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATTCTGCCAGGCTCAGCGGTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAGTCCCTTTAGTGTCCAGTCGTGCGTTGACACTTTTTTGATCGCTTTTATGTATTAcg                                                                                                                                                                                                           <  2:424194/251‑1 (MQ=255)                                                    ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGCCACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTcc                                                                                                                                                                                   >  2:351454/1‑251 (MQ=255)                                                      ctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTcc                                                                                                                                                                                   <  1:426133/249‑1 (MQ=255)                                                                                           aTTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGa                                                                                                                                              >  1:374084/1‑249 (MQ=255)                                                                                               gATTGTTTGCCCGCGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATa                                                                                                                                        <  2:289278/251‑1 (MQ=255)                                                                                                                      cAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTa                                                                                                                 <  1:253236/251‑1 (MQ=255)                                                                                                                       aaTACGCGATACTGGCGGCTGAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaa                                                                                                                <  1:97394/251‑1 (MQ=255)                                                                                                                       aaTACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGCAGCATAAAGCTGTTGTATGTTTGGTGTTa                                                                                                                 >  2:79426/1‑250 (MQ=255)                                                                                                                           cgcgATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaaaaa                                                                                                            <  1:58034/251‑1 (MQ=255)                                                                                                                              gATACTGGCGGCGTAGCAGTAAAAGGGACTGAAAAACTGCAAGGCCAAGAGTTGTCATCGCCAGTATCTAACCAACTATGGACACGCAACGGGTTATTACCTTTATCTTACAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGACCCCGAAATGACAATGTCCGCAATAGAAGATGAACTAAAAGCAAAACAGGTTACGACGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                                                                         >  1:198512/1‑251 (MQ=255)                                                                                                                                             gCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGAt                                                                                           >  1:150604/1‑250 (MQ=255)                                                                                                                                                        gACTGTAATTCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGTCGGGCGTTGACACTGTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTACAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAACAACATAAACCATCCCTGATAGAGttaattaa                                                                               <  1:152869/251‑1 (MQ=255)                                                                                                                                                                            cAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCATCGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGAGAGAGTTAATTAAAACAGGGAATAAGATaaa                                                             <  2:49867/249‑1 (MQ=255)                                                                                                                                                                                             gTATCTAACCAACTCTGGACAAGCAACGGGTTATTCACTTTATTTTCCAGACGGGCGTTGACACTTTTTTGATCGCTTTTATATAGTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACATGTTACGAAGCATAAAGCTGTTGTATGATTGGTGTGAAAAAACATAAACCATCCTTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTccc                                           <  2:33967/250‑1 (MQ=255)                                                                                                                                                                                                                     aaCGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAACCATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAAc                  >  1:257695/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAAtactt      >  2:132504/1‑251 (MQ=255)                                                                                                                                                                                                                                     tATTGTCCAGACGTGCGTTGACACTTTTTTGATCGCTTGTATATATTTCGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTAACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAAGTATTATTCCCTGTATATTCATTCAATCAATTTACCTGGTGAAttcttact   <  2:161565/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTg  >  1:45850/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             CTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATACTTACTG  >  NC_000913/155826‑156304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑