Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	97.7%	148.1 / ‑6.2	43	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/26);  minor base T (1/0);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCT  >  NC_000913/1829643‑1830110                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                ccttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑TCACCACGCGGAAATGGTGATAATTTTCATTAGCGAcc                                                                                                                                                                                                                           <  1:174952/251‑1 (MQ=255)           acacCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCTTTCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAAGCAAACGCTCGGTTGCCGGAGCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTg                                                                                                                                                                                                                 <  2:351474/251‑1 (MQ=255)                 cGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCACGGGTGCCGGGTCGGGACGCACCA‑GCACCACGAGGAAATGGGGATAATTTTCATTAGCGACCAAGCAGGCTGGGaaa                                                                                                                                                                                                            >  1:416416/1‑250 (MQ=255)                           cGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCA‑GCACCACGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTgcgc                                                                                                                                                                                                 >  1:221850/1‑251 (MQ=255)                               ttACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGCGGGCCTCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGAGCGGTACGCACCA‑GCACCACGCGGGAATGGGGATAAATTTCATTAGCGACCCGGGAGGCTGGGGAATCGCCCTGCGCAgac                                                                                                                                                                                             >  1:297330/1‑249 (MQ=255)                                     ttCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGGGGTAATTTTCATTAGCGACCAGGCAGGCCGGGAAAACGCCCTGCGCAGGCTGCCCt                                                                                                                                                                                       >  1:92800/1‑251 (MQ=255)                                         cacGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCCGGCAGGCTGGGAAATCGCCCTGCGCCGGCTGCCCTTCTg                                                                                                                                                                                   >  1:431135/1‑251 (MQ=255)                                          ccgcgATGCGCCCTGGCCCGTTATCCAGTCACCTTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGTCGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGGTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGc                                                                                                                                                                                  <  2:358448/250‑1 (MQ=255)                                              gATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGGAAACGCCCTGCGCAGGCTGCCCCTCTGCCACt                                                                                                                                                                              >  1:88399/1‑251 (MQ=255)                                                ggcgcCCTGGCCCGGTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCGCCTCTGTGCACAGGCGCACCAGACGAACGCGAGCCCCGGCGTGGCATTTGAGGGCATCAAGTTGGGCGGCGGTTAAAATCAAACGCTCGGTGGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATGTGCATGAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCACTTCTTCCACTTc                                                                                                                                                                            <  2:101026/250‑1 (MQ=255)                                                        ggCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCCCGGTTGCCGGATCGGTACGCACCC‑ACACCACCCGGAAATGGGGTTAATTTTCATTAGCGCCCAGGCCGGCTGGGAAATCGCCCCGCGCAGGCTGCCCTTTTGCCACTTCCACCCGcc                                                                                                                                                                    >  1:147967/1‑251 (MQ=255)                                                                                 ccATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAcgcg                                                                                                                                           <  2:222429/251‑1 (MQ=255)                                                                                        gTCATGCTGTTCTCTCCTCTACGAACCGGCGCACCAGACGACCGCGATCCCCGGAGTGGAATTTGAGGGCATCCAGTTGTGCGGCGGTTAAACTCACACGCTCGGTTGCCGGATAGGTCCGC‑CCACGACCCCCGCGGAAATGGTGATAATTTTCATTAGCGAACAGGCAGGCCGGGGAAACGCCCTGCGCCGGCTGCCCTTCTGCCACTTCCACCCGCCGACTTTTACGGCTGGGGCGCACGCgggctatg                                                                                                                                    >  2:258045/1‑245 (MQ=255)                                                                                             gCTGTTTTCTCCTCTGCGCCCAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTcaca                                                                                                                               <  2:88399/251‑1 (MQ=255)                                                                                                   ttcgcctcTGCGCACAGGCGCCCCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCaa                                                                                                                          <  2:185647/246‑1 (MQ=255)                                                                                                       cctcTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTGCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTc                                                                                                                     <  1:162945/251‑1 (MQ=255)                                                                                                          ctGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCCAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCCTTAGCGACCAGGCAGGCTGGGAAATCGCCCCGCGCAGGCTGCCCTTCTTCCCCCTCCACCAGCCGACCTTTACGGATGGGCCGCCCGCGGTCGCTGTCACCCTCCACCGTCGGc                                                                                                                  >  2:24678/1‑251 (MQ=255)                                                                                                                acaGGCGCACCAGACGAACGCTATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTTGGAAATCTCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAc                                                                                                                                              <  2:418619/217‑1 (MQ=255)                                                                                                                acaGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTAAGCACCA‑GCACCACGCGGGAATGGTGATAATTTTCATTTGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGCGCGCAc                                                                                                                                              >  1:418619/1‑217 (MQ=255)                                                                                                                      gcACCAGACGACCGCGATCCCCGGCGTGGCATGTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAg                                                                                                      <  2:97289/251‑1 (MQ=255)                                                                                                                            gACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCg                                                                                                <  1:156688/251‑1 (MQ=255)                                                                                                                                      aTCCCCGGCGTTTCATTGTAGGGTATCCAGTTTGGCGGCGGTTAAACTCAAACGCTCGGTTGCCTGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGTCTGGGAAATCGCCCTGCGCAGGCTGCCCTGCGGCCACTGCCACCAGCCGACTTTTACGGATGCCGCGCACGCGGTCGATGTCACCCTCAATCGGCGGCCCCCCGTCAAAGATGTCGATATAGtttc                                                                                      <  2:257340/251‑3 (MQ=255)                                                                                                                                       tCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCg                                                                                                                                                                   >  1:159031/1‑173 (MQ=255)                                                                                                                                       tCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCg                                                                                                                                                                   <  2:159031/173‑1 (MQ=255)                                                                                                                                       tCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGAACAGGCAGGCTGGGGAATCGACCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGGGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATTTAGTTACg                                                                                     >  1:181444/1‑251 (MQ=255)                                                                                                                                                  gctgttGAGGGCATGCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCTGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCTACCAGGCAGGCTGGGAAATCGCTCGGCTCAGGCCGCCCTTCTGCTACTTCCACCATCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTTACACTCAGGCGTCGGCCCACCGTCAAAGATGTCGATAGATTTACGGTGGCGAAAAc                                                                          <  2:153791/247‑1 (MQ=255)                                                                                                                                                      ttGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACctt                                                                       <  1:215272/250‑1 (MQ=255)                                                                                                                                                             cATCCAGGTGGGCGGCGTTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCGCCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCGTCTTTCTcc                                                               <  2:244657/251‑1 (MQ=255)                                                                                                                                                                    gtgtgCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑TCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCTCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCTGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAcc                                                         <  1:231776/249‑1 (MQ=255)                                                                                                                                                                     tgtgCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAATATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcg                                                        <  1:383400/250‑1 (MQ=255)                                                                                                                                                                      gtgCGGCGGTTAAAATCAAACGCTCGGTGGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCCCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgcg                                                      <  2:112823/251‑1 (MQ=255)                                                                                                                                                                              gTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAgg                                              <  1:368071/251‑1 (MQ=255)                                                                                                                                                                                tAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgc                                             <  2:431135/250‑1 (MQ=255)                                                                                                                                                                                           gCTCGGTTGCCGGAGCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCGCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGa                                 <  2:196120/251‑1 (MQ=255)                                                                                                                                                                                              cGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGTAAATCGCCCTGCGCAGGCTGCCCGTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAGGTCGATATAGTTGCGGTAGCGAAAACCTTCTTTCGCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAt                                <  1:390962/249‑1 (MQ=255)                                                                                                                                                                                                          cGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                  <  1:181557/251‑1 (MQ=255)                                                                                                                                                                                                          cGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                  <  1:325138/251‑1 (MQ=255)                                                                                                                                                                                                              aCGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGt              >  2:45804/1‑251 (MQ=255)                                                                                                                                                                                                                 cacca‑gcaccaCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATCGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACggccc           >  2:75809/1‑247 (MQ=255)                                                                                                                                                                                                                   cccacccccccgcgGAAATGGTGATAAGTTTCATCACCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAATCCGTCTTACTCCATCACCGCGCGGGCAGGCGCGGTTGGCGGAGGTCCCTGAACGAGGACGTCCTg          <  2:273724/241‑1 (MQ=255)                                                                                                                                                                                                                        caccacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCCGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCtt     >  1:245215/1‑251 (MQ=255)                                                                                                                                                                                                                           cacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGACATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCt  >  1:141988/1‑251 (MQ=255)                                                                                                                                                                                                                           cacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGCCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCt  >  2:187329/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                CCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCA‑GCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCT  >  NC_000913/1829643‑1830110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑