Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	82.9 / NA	25	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACTTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTG‑CTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652089‑1652575                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ggTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGATCATAGCA‑TTGATGTTCATCTCagaga                                                                                                                                                                                                                                                 <  2:404250/250‑1 (MQ=255)               tataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGcag‑aacag                                                                                                                                                                                                                                  <  1:315620/250‑1 (MQ=255)                    tGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGaaaaa                                                                                                                                                                                                                             >  1:144592/1‑250 (MQ=255)                      gAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAAAGAGACTGAGCCAAAAGGAAATCA‑TTGATGTTCACCCCAGAAGGTATGGCAG‑AACAGaaaaaac                                                                                                                                                                                                                           >  2:321096/1‑248 (MQ=255)                                  gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAACTGGAACAATGATGATCAGGAGAAATTTCGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGACCCAATCGGGGCAATCATATTTTTAAACCAGCAATCCACGCGCTCCGGAGCCATATGACATTTACAAGCCTGAGAAAAAAGACTATCA‑TTGATGGTTACCACCGAGAGAATGGCAG‑AAAAGAAAAACAAAAATACACCttt                                                                                                                                                                                                              >  1:374160/1‑251 (MQ=255)                                                                tAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt                                                                                                                                                                                 >  1:83018/1‑250 (MQ=255)                                                                       gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaa                                                                                                                                                                            <  2:144592/248‑1 (MQ=255)                                                                       gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAtt                                                                                                                                                                         <  2:362661/251‑1 (MQ=255)                                                                        aaaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGCCCGTTTAACGGTACCAAAAACACAGGCGGGGACCAATCGGGGCATTCATACTATTAAGCCAGCAATCGACGCCCTCCCGAGGCAGATGACAATTATAAGACTGGACAAAAAGCATCTCAATTG‑TGTTCACCTCCGAGGGTATAGCAGGAAC‑GAAAAAAAAAAATGAACCTTTTTTTTTTCACCTGGAATGTCAGCGAGGGGAAAAAAtc                                                                                                                                                                         >  2:417616/1‑249 (MQ=255)                                                                           aaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATATTATTAAGCCAGCAATAGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAGAGTCAGCGAGAGTAAAAAATTATgg                                                                                                                                                                     >  1:153838/1‑251 (MQ=255)                                                                                aaCAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGAACTTAAAGAGAATGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGCGAGAGGAAAAAATTATGGTGAcc                                                                                                                                                                >  1:214500/1‑251 (MQ=255)                                                                                                      ttAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATaa                                                                                                                                           <  1:321096/250‑1 (MQ=255)                                                                                                                             aCCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATaa                                                                                                                   <  1:317502/251‑1 (MQ=255)                                                                                                                                       caGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAGGTGTGACGCAGCGATAAAACGTGCCgg                                                                                                         <  2:277502/251‑1 (MQ=255)                                                                                                                                            gggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACGCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcg                                                                                                    <  2:82120/251‑2 (MQ=255)                                                                                                                                                 ccAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACTCAGCGATAAAACGTGCCGGACTCCGCCAt                                                                                               <  2:55009/251‑1 (MQ=255)                                                                                                                                                        ggTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATACAACGTGCCGGACTCCGCCATCGAAAAt                                                                                        >  2:103150/1‑251 (MQ=255)                                                                                                                                                                         aGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCAGCGAAAATCATATCAGTCGAGACAt                                                                       <  2:153838/251‑1 (MQ=255)                                                                                                                                                                                                 gTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGAc                                                >  1:67300/1‑250 (MQ=255)                                                                                                                                                                                                     gATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCAAGACATACTTATGCCTGCTGGTCGCTGACAGCTg                                           >  2:226620/1‑251 (MQ=255)                                                                                                                                                                                                             tAACGAGACTGAGCCAAGAGAATAGCA‑TTGATATTCACCTAAGAGAGTATGGCAG‑AACAGAAAAACAACAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGAAGATGTGGGACGCAGCGATACAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATAATTATGCCTGCTGGTCGCTGAAAGATGGGG‑CTaa                                   >  2:302516/1‑250 (MQ=255)                                                                                                                                                                                                                                           gATGTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTG‑CTAACCCGGCATTTATAGCAAACCAGATGGGcc      >  1:189796/1‑250 (MQ=255)                                                                                                                                                                                                                                            atgatCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGCGACAGCGAAAGTAAAAAATTATGGAGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACCTACTTATGCCTGCTGGTCGCTGACAGCTG‑TGTCTAACCCGGCATTTATAGCAAACCAGATGAGCCAt    >  2:110050/5‑251 (MQ=255)                                                                                                                                                                                                                                              gTTCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTG‑CTAACCCGGCATTTATAGCAAACCAGATGGGCCATGc  >  1:297168/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttCACCTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTG‑CTAACCCGGCATTTATAGCAAACCAGATGGGCCATGc  >  1:377410/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCA‑TTGATGTTCACTTCAGAGAGTATGGCAG‑AACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTG‑CTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652089‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑