Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	97.9%	149.8 / NA	47	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (22/24);  minor base G (0/1);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTC  >  NC_000913/1643440‑1643921                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    gatgatGCGGATCGTCGGCTGGCATACCGCAGAAAGCACACGGCTGTGTCTTACCCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGt                                                                                                                                                                                                                                          >  1:360796/1‑251 (MQ=255)              gtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTTGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCttt                                                                                                                                                                                                                             <  1:372822/251‑1 (MQ=255)                       cattccGCAGCAAGCTCACGGCTTTGTCTTAACCCAACGTGTGTATTTCTCCGTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGAGTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTTCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCAGTCTGCTCTCCCGGTACGATGGCGCTTTCGCGGTaca                                                                                                                                                                                                                    <  2:296787/247‑1 (MQ=255)                       cATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTac                                                                                                                                                                                                                     >  1:157564/1‑250 (MQ=255)                          aCCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCg                                                                                                                                                                                                                 <  2:213502/251‑1 (MQ=255)                             gcagcaAGAACACGGCTGTGTCTTAACCCAAAGCGTGCATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGAATCTGGATCAACGGTGATGCTTACCACAGTCTTTTCATGTGGGGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCCCCCGGTACGATGTCGCTTTCGCGGTACACCGAGc                                                                                                                                                                                                              >  1:26059/1‑251 (MQ=255)                               agcaagcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCTAGCAg                                                                                                                                                                                                            <  1:133342/251‑1 (MQ=255)                                       cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGCGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTc                                                                                                                                                                                                    >  1:28504/1‑251 (MQ=255)                                        acGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGGCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAATGCAACGGTGCAAGATGTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCtctc                                                                                                                                                                                                                                               <  1:32388/207‑1 (MQ=255)                                        acGGCTGGGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCtctc                                                                                                                                                                                                                                               >  2:32388/1‑207 (MQ=255)                                                    tAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAAt                                                                                                                                                                                          <  2:177372/248‑1 (MQ=255)                                                     aaCCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTGTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCa                                                                                                                                                                                      <  2:208157/251‑1 (MQ=255)                                                           aCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTGTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAAGCCCAGCGAac                                                                                                                                                                                <  2:250559/251‑1 (MQ=255)                                                            cGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAacg                                                                                                                                                                               <  1:177522/251‑1 (MQ=255)                                                            cGTGTGTATTTCGCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTGTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGGGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGGCGCTTTCGCGGTACAGCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAacg                                                                                                                                                                               <  1:403775/251‑1 (MQ=255)                                                                                ccAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTCCCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCCGCGAACGACGCGATACAGCCTCAGGt                                                                                                                                                            >  2:212882/1‑250 (MQ=255)                                                                                                             aaGATTCCGGAGCCTCTGGCTCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGAGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGACCGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGcc                                                                                                                              >  2:22549/1‑251 (MQ=255)                                                                                                              aGATTCCGGCGACTCTGGATCAACGGTGATGCTTACCCCCGTCTTTTCCTGTGGTGCTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGCTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAgcacgccc                                                                                                                             >  2:339300/1‑244 (MQ=255)                                                                                                                   ccGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTTGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTTCACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAg                                                                                                                        <  1:325762/251‑1 (MQ=255)                                                                                                                    cGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCGGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCGCCAgg                                                                                                                       <  1:345451/251‑1 (MQ=255)                                                                                                                                     cGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCTGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGGCCGCCCACCAGGATAATTCAGCCAAAGAt                                                                                                      <  1:1018/251‑1 (MQ=255)                                                                                                                                     cGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCTACCAGGATAATTCAGCCAAAGAt                                                                                                      <  2:87196/251‑1 (MQ=255)                                                                                                                                         gATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAAt                                                                                                   <  1:219133/250‑1 (MQ=255)                                                                                                                                            gCTTACCTCCGTCTTTTCCTGTTGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTGCAATATGCTGTTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCGCCACCTGATTGCAGACCGGCCACCAGGATAATTCAGCCAAAGATAATTcc                                                                                                <  1:381872/250‑1 (MQ=255)                                                                                                                                            gCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTcc                                                                                                <  2:157564/250‑1 (MQ=255)                                                                                                                                             cTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCg                                                                                              >  1:165079/1‑251 (MQ=255)                                                                                                                                              ttACCACCGTCTTTTCCTGTGGTGATTTTTGTTAATGGTGGGCGTAAGGCCACGGAGCCCGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGc                                                                                             >  1:181189/1‑251 (MQ=255)                                                                                                                                                 ccaccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTcc                                                                                          <  1:238333/251‑1 (MQ=255)                                                                                                                                                   cccGTCTTTTCCTGTGGTGATTGTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTg                                                                                        <  2:188727/250‑1 (MQ=255)                                                                                                                                                                            gttgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTAACGGAt                                                                <  1:115793/250‑1 (MQ=255)                                                                                                                                                                                 tggtggGAGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGCGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTc                                                          >  2:204567/1‑251 (MQ=255)                                                                                                                                                                                  ggtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGt                                                           <  2:79567/249‑1 (MQ=255)                                                                                                                                                                                        cGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCccacca                                                                                                                         >  2:232174/1‑181 (MQ=255)                                                                                                                                                                                        cGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCccacca                                                                                                                         <  1:232174/181‑1 (MQ=255)                                                                                                                                                                                          tAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑Accc                                                 >  1:20017/1‑250 (MQ=255)                                                                                                                                                                                              gCAACGGTGCAAGATGTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGAGTTCAGACCGCCCGCCAGGATAATTCAGCCAAAGATAATTCCCGCTCGTGGGGACCGCTTAGTGCGGGACTGATGCCGTCAATC‑ACCAATGCt                                            <  2:60210/251‑1 (MQ=255)                                                                                                                                                                                               cAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTg                                           >  1:412866/1‑251 (MQ=255)                                                                                                                                                                                                 aCGGTGGAAGAGTTGTTGTGCGTTGTTTCAATATGCGGGTGGCGGTCTGCTCTCCCGGTACGATGGCGCTTGCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATGCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑GCCCATGCTGTc                                         <  2:167986/251‑1 (MQ=255)                                                                                                                                                                                                             tttttGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTttgttg                              >  1:125866/1‑250 (MQ=255)                                                                                                                                                                                                                  gtgCGTTGTTTCCATATGCTGGTGGAGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCCTCCACCACCTGCTTGCAGACCGCCCCCCAAGATAATTCAGCCAAAAAAACTTCCAGCTCCTGAGTACCGCTTATTGCGTGCCGGCTGACGTCAATCCACCC‑TCCTTTCAGATTTTGTTGtgcacg                        >  2:248296/1‑249 (MQ=255)                                                                                                                                                                                                                          tttCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTcc                 >  1:332348/1‑250 (MQ=255)                                                                                                                                                                                                                                 atGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCAGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAACGAACGACGCGATACAGCCTCAGGTACTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTc         >  1:177202/1‑251 (MQ=255)                                                                                                                                                                                                                                  tGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCCGATTTTTTCCGCTGGTAATCCCAGCGAACTACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGCTTGCAGACCGCCCACCCGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTCTTGCGTGACGGATGACGTCAATC‑ACCCACGCTGTCAGATTTTGTTGAGCACGCAGCTCCAGTGATTcc        >  1:289601/1‑251 (MQ=255)                                                                                                                                                                                                                                     tggtggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGCACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCCCCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGAt     >  1:418436/1‑251 (MQ=255)                                                                                                                                                                                                                                       gtggCGGTCTGATCTCCCGGTACGATGGCGCTTTCGCGGTCCACCGAGCAGGTTTTTTCCGCTGGTTATCCCAGCGAACGACGCGATACAGCCTCACGGAGTGCATCCACCACCTGATTGCAGACCGCCCCCCAGGATAATTCAGCCCCAGATAATTCCCGCTCCTGCGTACCGCGTATTGCGGGACGGATTACGTCCATC‑CCCCATGCCGTCAGATTTTTTTGAGCAAGCCGCTCCAGTGATTCCgcagg   >  2:295425/1‑247 (MQ=255)                                                                                                                                                                                                                                        tggCGGTCTGCCCTCACGATACGATGCCGCTTTCCCGGTACCCCGAGCAGATTTTCTCCGCTGGTAATCCCAGCGACCGACGAGATCCAGCCTCAGGTAGTGCATCAACCACCTGATTGAAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGAGTACCGCTTCTTGCGTGACGGCTGACGTCACTA‑ACCCATGCTGTCAGCTTTTGATGCGCAAGCAGCTCCCGTGCTTCCGAAGTc  >  2:251508/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTc  <  2:9616/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    GATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATC‑ACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTC  >  NC_000913/1643440‑1643921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑