Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,674	G→A	G282R (GGG→AGG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,674	0	G	A	100.0%	46.9 / NA	16	G282R (GGG→AGG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

TGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGC  >  NC_000913/1336436‑1336892                                                                                                                                                                                                                                               |                                                                                                                                                                                                                           tGCTTATCCGGATACATTCGTTGGTACTGCCTCGCACACCCCCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATtcgtc                                                                                                                                                                                                                 <  1:327201/250‑1 (MQ=255)                        gTACTGACTCGCACACCACCATGCAAAACGGCCTTGGCGTGCTGGGGGGGGGCGTTGGTGGGATCGCAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCTTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAGAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCCCACTGTTACCCAAATACTGCGAAAACATGCCGTGGTGAGGAAATTCGACGAATTTTATGGTGATGGCCTGGAt                                                                                                                                                                                         >  2:2133/1‑251 (MQ=255)                                          ccattagaaaCGGCCTTGGTGTGCGGGGGGGGGGCGTTGGGGGGATCCAAGCAGAAGCCGCAAGGTTAGGCCTGCCTGTTTCCATGCTTATCCGGGATGTAGGGGGCCTCAACATTCCCGTAAATTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCGCTGTTAACCAAATGCTGCGCAAACATGGCGGGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACAACCGttttcggat                                                                                                                                                                       <  2:350621/243‑8 (MQ=255)                                                                                   gggATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACACAAATGATGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGTATCGCGCCACCATTGCCAATATGTCGCCAGAATCTGGTGCCAc                                                                                                                              >  1:230826/1‑251 (MQ=255)                                                                                                            tAGGCCAGCCGGTTTCCATGCTTAGCCCGGATGTAGTTGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGc                                                                                                      <  1:403633/250‑1 (MQ=255)                                                                                                                                     cgcggaggtagggtgCTTCAAACTTACCGGAAAAGTACGTGAAGGTATTTCCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATTGTCTGGAGTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTa                                                                             <  2:351785/236‑1 (MQ=255)                                                                                                                                      ccGGATGTAGCGGGCTTCAAACTTACCGGAAACTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACCTGGCGTGGTGAGGAAATTCGTCGCATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGACAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTa                                                                             >  2:165061/1‑249 (MQ=255)                                                                                                                                       cGGATGTATTGGGCTTCAAACTTACCGGAATATTTCGGGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc                                                                          <  1:137604/251‑1 (MQ=255)                                                                                                                                        ggATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCg                                                                         >  2:24726/1‑251 (MQ=255)                                                                                                                                              aGTGGGCTCAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACGACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCGGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                                                                    <  1:77044/250‑1 (MQ=255)                                                                                                                                                                       ttACGGGAATGTATGACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                           <  1:109283/250‑1 (MQ=255)                                                                                                                                                                                   aTTACCGCCACAGACCTGGGTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCa                               <  2:381431/250‑1 (MQ=255)                                                                                                                                                                                      aCCGCCACAGAACTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACTGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAAATCAGGTCGAGTTGGTCGAAAAATATGCCAAAgc                           >  2:395971/1‑251 (MQ=255)                                                                                                                                                                                                    ggTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGc             <  1:11261/251‑1 (MQ=255)                                                                                                                                                                                                         gcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAAGTCGTCGAATTTTATGGTGATTGTCTGGATGCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGGGCCACCTGGGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCATGGCATGTGTCGTAAc        <  2:390695/250‑1 (MQ=255)                                                                                                                                                                                                               ttaccccaacactacgcAAACATGGCGTGGTGAGGCAATTCGTCGAGTTTTATGGTGATGGTCAGGATACACTACCGTTGGAGGATAGAGCCACAATTGCAAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCAAATCGATGCTGTAAAACTCCATTACATGCGTTTAAGCGGGCGCAGCGAAGATTAGGTCGAGTTAGTCGAACAATATGCCACAGCGCAGGGCCTGTTGCGTAACCCggcc  >  2:346710/15‑249 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                           TGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGC  >  NC_000913/1336436‑1336892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑