Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	52.7 / NA	17	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/8);  total (9/8)

GAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAA  >  NC_000913/1301776‑1302237                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gagaAATTCGGCGAAAAATGGACCCAGCCTGGTACTAGCGTCACCAACGGTGCGTATACCTTAAAAGATGTGGTCGTAAACGAACGAATCGTTCTGGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATGAACCAGTTAACCGATTTGCCGATGTCATCTGTAGGTACCTACGTCAGCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACTGCATGCCTATGGAATTGTCGCAGAAgtt                                                                                                                                                                                                                     <  2:344324/251‑3 (MQ=255)  agaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGACGACCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAAAATATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACAGAAGTGGGGAAATCGCAATGACGTATAACAGCAGGCCGCTCGAATTGTTCCAGAAGCt                                                                                                                                                                                                                     >  1:355139/1‑250 (MQ=255)  agaAATCCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                                    >  1:118593/1‑251 (MQ=255)   gaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGACCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                                    >  1:176991/1‑250 (MQ=255)     aaTTCGGCGAAAAATGGAACCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaa                                                                                                                                                                                                                  >  1:96385/1‑250 (MQ=255)      attcgctgAAAAATGGACCAAGCCTGGAAATATAGTCACCAACCGTGCCTATACCTTAAAAGAATGGGTCGAAAACGAACGCATCGTACTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGAAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCAAGAAGCTGaaa                                                                                                                                                                                                                 >  2:278986/8‑250 (MQ=255)                                         cctccAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTGCACGTTGATCCATAc                                                                                                                                                                             <  2:45089/248‑1 (MQ=255)                                                                       ggggcGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGGAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCTAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGTCGAATTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaa                                                                                                                                                <  1:159787/246‑1 (MQ=255)                                                                                                  aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGTTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCACCCGCTACCGTATTGGTGAAATCGACATGACGTATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTCCGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgt                                                                                                                    <  2:210745/251‑1 (MQ=255)                                                                                                                        caacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAg                                                                                              <  1:302782/251‑1 (MQ=255)                                                                                                                                         ttaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATc                                                                             >  2:154763/1‑251 (MQ=255)                                                                                                                                           aaCCTGGTAACCTATGTGCATCTGGCTTCTGAAGTTACAGCTGTCACCCGCTACCGTGGTGGTGAAATCGACATGGCTTATATCCGCAAGCCGATCGAATTTTTCCAGATGATGAAAAAAGAGATCCCGATCGAAGTACACGTGGATCAGTCCCTGTGCACTTACTTTTACGAAATTATCAACAAAAAACCGCCATTCAACGATGTGCGTGTGCGTACTGCACTGAAACTAGGTATGGACCGCGATATCag                                                                           <  2:355139/251‑2 (MQ=255)                                                                                                                                                                       cTGAAGGTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAggtgatgctgccaacttactgatttagtg                                               >  1:113743‑M1/1‑222 (MQ=255)                                                                                                                                                                         gAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAggtgatgctgccaacttactgatttagtgtt                                             >  1:303620‑M1/1‑220 (MQ=255)                                                                                                                                                                                              tACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgct                        >  2:111188‑M1/1‑199 (MQ=255)                                                                                                                                                                                                          gAAATCGACAGGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTCCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttct            <  2:118593‑M1/251‑65 (MQ=255)                                                                                                                                                                                                                     gACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatca  <  1:300393‑M1/250‑75 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAA  >  NC_000913/1301776‑1302237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑