Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,980	A→G	T156T (ACT→ACC)	phoP ←	DNA‑binding transcriptional dual regulator PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,980	0	A	G	100.0%	55.4 / NA	19	T156T (ACT→ACC)	phoP	DNA‑binding transcriptional dual regulator PhoP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

ACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTC  >  NC_000913/1189733‑1190204                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 ccccGCAGCGAGAGCTGGAAAAACAGTCGCAGTAATTGTTTCATCAGCGCAATTCGAACAGGTAGCCCTGGCCGCGAACTGTGGTAATCACTTCTTGGGGATATTGTTCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATTGCTTTCCCGCAGCTCCGCATCCGGATAGAGTGGGATCACTAACGAATCTTTGCTGACCACGTTGCCATTATGGCGTATCAACGTTTCCATAATGgtg                                                                                                                                                                                                                               <  2:385478/250‑1 (MQ=255)    cGCAGCGAGAGCGGGAAAAAAAGACGCAGAAATTTTTTCCTCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGCTAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTAt                                                                                                                                                                                                                            >  2:114612/1‑251 (MQ=255)                         agacgaaatAATTTTTTCATCAGCGCAAATCGAACAGATAGCCCTTGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTACGCAGAAGTCCCATCAGTACATCCTTGGTCTGGCTTTCCCGCAGCTCCGAATCCGGATAGAGTTGGAGAATTAACGAATCTTTGCTGACCACTTTGACCTTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATc                                                                                                                                                                                                       >  2:354735/9‑251 (MQ=255)                            cGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACt                                                                                                                                                                                                    >  2:97520/1‑251 (MQ=255)                               aGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATAACTTCTTGGGGCTATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCCTTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCg                                                                                                                                                                                                 >  1:184189/1‑251 (MQ=255)                                         tcatcaGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAAt                                                                                                                                                                                         >  1:86414/1‑249 (MQ=255)                                                    aTTCGAACAGATAGCCCTGGCCGCGTAAGGTGGTAATAACTTATTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTATGGCTTTCCCGCAGATCCGCATCCGGATAGAGTTGGAGCATTAACGACTCTTTGATGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGCCATTAATAGATAATTCACgg                                                                                                                                                                            >  2:254990/1‑251 (MQ=255)                                                                                 gtggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGCCGTCCCATCAGTACATCCATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACgggggg                                                                                                                                                 >  2:204346/1‑249 (MQ=255)                                                                                      aaTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGAATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATAAACCTGAACCGGGGGGAGCGaa                                                                                                                                           >  2:286401/1‑250 (MQ=255)                                                                                                  gggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACAACATTGGTATGACTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCCTTATTGCGTGTCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGCTTGATCACTTCGTCATTAATAGATAATTCACGGAGAGAGAGATCAACCTGAACCGGTGGGAGCGAAATGACCTGTGa                                                                                                                                >  1:68891/1‑249 (MQ=255)                                                                                                                gAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTAt                                                                                                                  <  1:114612/249‑1 (MQ=255)                                                                                                                          gcgcAGACGTCCCATCAGTACATCAATGGTATGTCTTTCCCGCAGCGCCGCATCCGGATAGAGTTGGAGCATTAACGAATCGTTGCTGACCACTTTGCCATGATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATGAATAGAGAATTCACGTCGAGAGAGATCAACTTGAAACGGGGGGAGCTCAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTa                                                                                                      <  1:53706/251‑1 (MQ=255)                                                                                                                                                      gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTc                                                                          <  2:86414/251‑1 (MQ=255)                                                                                                                                                         tGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTCATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTATTCAat                                                                       >  2:66767/1‑251 (MQ=255)                                                                                                                                                                            cATCCGGATAGAGTTGGAGCATTAACGAATCTATGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcac                                                     >  1:318398/1‑250 (MQ=255)                                                                                                                                                                                                            ttGCTGACCCCTTTGCCATTATTGCGTATCAACTTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCTCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCTCCGGCACTTAATACTTc                       <  2:184189/248‑1 (MQ=255)                                                                                                                                                                                                             tGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTGCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGAc                    <  1:369490/250‑1 (MQ=255)                                                                                                                                                                                                                            cATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCtt    <  1:218397/251‑1 (MQ=255)                                                                                                                                                                                                                               tattGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTc  <  1:97520/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 ACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTC  >  NC_000913/1189733‑1190204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑