Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,148,972 C→T Q72* (CAG→TAG)  fabH → 3‑oxoacyl‑[acyl carrier protein] synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,148,9720CT94.4% 54.7 / NA 18Q72* (CAG→TAG) fabH3‑oxoacyl‑[acyl carrier protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/8);  minor base G (0/1);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGATC  >  NC_000913/1148745‑1149209
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
gTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCa                                                                                                                                                                                                                        <  1:308406/251‑1 (MQ=255)
                                        cAGCTATCTGCACGACCCAGTGCGGACAACCGACGATTTGGCAAACATGGTGGACACCTCTGACGCGCGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGCGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTc                                                                                                                                                                                >  2:207537/1‑251 (MQ=255)
                                                                      cGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGtt                                                                                                                                                  <  2:263763/251‑1 (MQ=255)
                                                                          gATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAg                                                                                                                                                                                           <  1:135850/206‑1 (MQ=255)
                                                                          gATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAg                                                                                                                                                                                           >  2:135850/1‑206 (MQ=255)
                                                                               ggAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACAGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGCTTGGCCTGATCGTTGTGGCAACGCCTTCTGCTACGCACGCTTTCCCGAGCGCAGCGTGGCAGATTCAAAGCATGTTGGGCCTTAAAGGTTGCCCGGcgt                                                                                                                                         >  2:87881/1‑249 (MQ=255)
                                                                                  aaaaaTGGTGGGCACCTCTGACGAGTGGATTGTCACTCTTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACGAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTg                                                                                                                                      <  1:272294/251‑1 (MQ=255)
                                                                                  aaaaaTGGTGGACCCCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTg                                                                                                                                      <  2:298506/251‑1 (MQ=255)
                                                                                            gACACCTCTGACGAGTGGATTGTCACTCGTACCGGTACCCGCGAACGCCCCATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGCGAAACACTAGATTGGCCTGACAGTTGTGGCAACGACTTCTGCTAAGCCCGCTTTCCCGAGCGCAGCTTGACAGATTCAAAGCATGTTGTACATTAAAGGGTGCCAGGCATTTgccctttccgc                                                                                                                            >  2:365525/1‑241 (MQ=255)
                                                                                                                                                              gAAACCGTTGCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGaa                                                          <  1:27114/251‑1 (MQ=255)
                                                                                                                                                                     tttCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGCGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTCAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGGATGct                                                   >  2:71674/1‑251 (MQ=255)
                                                                                                                                                                     tttCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTATGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCCGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGGAGtgtgat                                                   >  1:185871/1‑246 (MQ=255)
                                                                                                                                                                                ggCTTTTAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGGCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTTCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCgg                                        <  1:287480/251‑1 (MQ=255)
                                                                                                                                                                                      gAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGCTTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTTTGGTCGTCTGTTCCgg                                  >  2:30011/1‑250 (MQ=255)
                                                                                                                                                                                              gACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTcc                                    <  1:98400/241‑1 (MQ=255)
                                                                                                                                                                                              gACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTcc                                    >  2:98400/1‑241 (MQ=255)
                                                                                                                                                                                                  cgcgCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGACTGCTCGTTGTGGCAACGCCTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAACGGTTGCCCGGCCTTTGCCGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGGAATCTGGGGCGGTGAAGTAAGCTATGGGCGTCGGTTCCGTTGTCCTGgcgcc                      >  2:390214/1‑250 (MQ=255)
                                                                                                                                                                                                                      cATTGAGAAAGACTATATGGGCCTGATCGTGGTGGCAACTACGTCTGCTACGCACGCTTTCCCGAGCGCAGCTTTTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGTCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCTATCAATACGTGAAATCTGGGGCGGTGGAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGATc  <  2:156797/251‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
GTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGATC  >  NC_000913/1148745‑1149209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑