| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,001,251 | A→C | Q679P (CAA→CCA) | elfC → | putative fimbrial usher protein ElfC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,001,251 | 0 | A | C | 92.3% | 72.8 / ‑5.5 | 26 | Q679P (CAA→CCA) | elfC | putative fimbrial usher protein ElfC |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (11/13); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TATACCAGGAAAAAAAATTACGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATC > NC_000913/1001007‑1001487 | tataCCAGGAAAAAAAATTACGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGa > 1:238923/1‑250 (MQ=255) cAGGAAAAAAAATTACGCCTGGATGACCTCTCAAC‑CCAGTAACAATCCCCCAGAGCACACTACACAAAAACAGGGTTTAACGGAGACCTTACTCCATACCGCAAACCTGCCCTACAGCGAGCAACAGAGCTATAACAGCGAGGGGAAAACCGCTACTGGTAGCGCCAACATGGACTACAAAGGGGAGTTTACGCACGCCAGAGTGGGCTACAACTACATCGATAACGTCAGACAACAACCACTGAACTaac > 1:59614/1‑249 (MQ=255) gAAAAAAAATTACGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGGTATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGctc < 2:7500/251‑1 (MQ=255) ttACGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGc > 1:221578/1‑250 (MQ=255) aCGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAATCCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACATGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGt < 1:217280/251‑1 (MQ=255) cTGGATGACCTCT‑AACACCGGTATCGATAACGAAGGGCACACTACAAAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCACCAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTCCGCTCTTTCAGGCCGttt > 2:50432/1‑249 (MQ=255) gATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTg < 1:363507/251‑1 (MQ=255) cgct‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTGTAACGGAGACATTACTCGATGACGGGAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGGAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCGCGAGTGGGCTACAACTACAGCGATAACGGCATTGAACAACCACTGAACGACGCTCTTTCAGGcgtaatgttttttcctg < 2:59614/249‑17 (MQ=255) aTAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTg > 2:334634/1‑251 (MQ=255) acacAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGa < 1:334634/251‑1 (MQ=255) tGGGTTTACCGGAGTCATTCCTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTACTGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCACCAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGtt > 2:254699/1‑251 (MQ=255) ccGGTAACCTGAGCTACAGCGTGCAACGGGGATATAACAACGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAACGGGGCGTTTGCGGAGGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCGACAACCACTGAACTACGCTCTTTCAGTCAGTTGAGTTGCCCATTCACAGGGCATTACCCTGGGGCAAGCGCTGGGGTAAACGAACTTTCTGATTGCAGCACCAGGCGCAGAGAAt < 2:385639/250‑1 (MQ=255) aCCTGAGCGACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCgt < 2:104620/251‑1 (MQ=255) aCAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAactgac < 1:50432/251‑1 (MQ=255) gCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGTCGTTTGCGGACGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTgg < 1:47428/251‑1 (MQ=255) gCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGata < 1:47233/245‑1 (MQ=255) gCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGata > 2:47233/1‑245 (MQ=255) gTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGCTATACCGTTGTTCCt > 1:50861/1‑251 (MQ=255) cATGGATCACAAAGGGGAGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCActt > 2:26790/1‑251 (MQ=255) aCAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCgg < 1:42815/251‑1 (MQ=255) ggCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAaatc > 1:131226/1‑251 (MQ=255) aCAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGtt < 1:402140/250‑1 (MQ=255) aCAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCGTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGcgtcgt < 2:208679/249‑1 (MQ=255) aCAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACGTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAaac < 1:254699/249‑1 (MQ=255) aGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAAGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACCGCACCGGGCTGAAAACTGACTGGCGCGGCTATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAaacgta > 1:252477/1‑250 (MQ=255) cGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGAAGTTTAGTTGCCCATTAACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGTTTGCAGCACCAGGCGCAGAGCATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTGGTTCCTTATGCCGCTTCTTATCGGGAAAATCGACTCGCACTTGATGCGGCGACGTTAAaacgtaactgggatc > 1:11396/1‑244 (MQ=255) | TATACCAGGAAAAAAAATTACGCCTGGATGACCTCT‑AACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATC > NC_000913/1001007‑1001487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |