| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,384 | 0 | T | G | 29.2% | 73.3 / 10.0 | 51 | V35G (GTG→GGG) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base T (10/24); new base G (14/0); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.13e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACG > NC_000913/119149‑119632 | aCTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATAATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑TAACATTTTtttct > 2:263294/1‑251 (MQ=255) agccGGATAACGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAGCCGATCCCGGTCCTGTATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATGTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCATCTTGAGCATCGTCTTGAAGCGTTCTTTCGGCGCGTG‑AGACATTTTTCTCTCGGGCGTACGTTa < 2:63675/248‑1 (MQ=255) ccGGATATCGCTAAAAACATGACTGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTGCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTTGGCGAGCACTGGCAGCTTTATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAgg < 2:302566/250‑1 (MQ=255) ccGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGc < 2:224318/251‑1 (MQ=255) atatCGCTAAAAACATGACGGGCCATTGTGCTATTGCGCCGGATAGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTCCTGGTGTTAATTTTCGCGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGCTCATCGTCTTGCAGCGTTCTTTCGGCGGGTG‑AGAAATTTTTTTATCGGGCTCACGTTTGGC‑ATgg > 1:192528/1‑250 (MQ=255) atCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCTGGCGCACGTTAGGC‑ATG‑A‑cc > 1:345621/1‑251 (MQ=255) gactggCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATgg < 1:383873/246‑1 (MQ=255) gggCCATTGTGATATTGCGCCGGATCGGACAACCGATCCCGGTCCTGCATTTGCTTGGGCACGGTTTCGTGTGCTGGTCAGCAGGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTTTCTTGTGCGCCCGTTAGGC‑ATG‑T‑ACattatttaggatggggg > 2:30085/1‑240 (MQ=255) tGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCGGGCGCCCGTTAGGC‑ATG‑A‑CCATTTTTTCGATGGGCGGG‑TGTTTTTTAATGtt > 2:221099/1‑251 (MQ=255) gcgcCGGCTCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACACCATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTTTCGGGCGCACCTTTGGCCATG‑‑‑CCCTTTTTTCGGTGGGGGTG‑ACTTTTTTTCTGttt > 2:175395/1‑250 (MQ=255) aTCGGAAAACCGATCCCTGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTTTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCTCTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCTCGGTAGGC‑ATG‑A‑CCATTATTGCTATGGGCGTG‑ACTTTTTTACTGTTACGCGCAt < 1:170338/251‑1 (MQ=255) cGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCAtt < 1:105030/250‑1 (MQ=255) aaCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACAATTTTTTCTCGGGGGCCCGGTTGGC‑ATT‑A‑CCATTATTGCGGTGGGGGTG‑TGTTTTTTTTTGTTACCAGCATTTCAggg > 2:231923/1‑251 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTa < 2:400374/251‑1 (MQ=255) cccGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTAtt < 1:339902/250‑1 (MQ=255) cGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGGTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑AGTTTTTTACTGTTACGCGCATTGCATGGAGTATTGtt < 2:44326/251‑1 (MQ=255) aaTTTGATTGGGCACGGTTTCGTGTGCTGTTCAGCAAGGAGACAACATGTCGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTTTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGGGTT‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTc < 1:175018/250‑1 (MQ=255) aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTcc < 2:20250/251‑1 (MQ=255) aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTcc < 1:257096/251‑1 (MQ=255) ttGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGCGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTTTTTCGGGCGCACGTTAGGC‑ATGCA‑CAATT‑TTGGGATGGGGGTG‑AGTTTTTTTCTGTTAAGAGCATTTCAGGGGGGATTGtttatagtttccc > 2:267076/1‑240 (MQ=255) ggTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTAACACATTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAG‑AAACATTTTTCTCTCGGGCGCACGCTAGAC‑ATG‑A‑CCCTTATTGCGATGGGCGTG‑ACTTTTTTACTTTTAAGCGCATTGCAGGGAGTATTTTTTAACCATCCCACGCTCCTGgtgg > 1:409410/1‑250 (MQ=255) gCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTACTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGCG‑AAACATTTTTCTTTCGGGGGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTAAGCGCATTGCAGGGAGTATTGTTCAACGTTCCCCCGCTACTGGTGTGgctgcttat > 1:38317/1‑248 (MQ=255) ggtcagcgtggtgaCAACATGACGCTATTTGCAACCTTACTGGTGTTAATCTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCGCGTTAGGC‑ATG‑A‑CGATTATTGCGATGTGCGTG‑ATTTTTGTACTGTTACGCGCATTGCAGTGAGTATTGTTCAACGTTCCGACGCTACTGGTGTAGCTTCTGATTgg < 2:253752/239‑1 (MQ=255) ggTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑AACCAT‑ATTGCGGTGGGCGTG‑ACTTTTTTACTGTTACGCGCCTTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTTGTGTGGCTGCTGATTgg > 1:206765/1‑251 (MQ=255) gaCAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAG‑AAACATTTTTCTCTCGGGCGCGAGATAGGG‑AAG‑A‑ACCTTTTTGTGGTGGGAGTG‑GATTTTTTTCTGGTACACGCATTACAGGGTGTATTGTTTAACATTCCCCAGCTACTGGGGAGGCGTCTGAATGGTTTGCTgtgtct > 1:180994/1‑249 (MQ=255) caTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgcg < 1:325890/251‑1 (MQ=255) cGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCCTTATTGCGATGGGCGGG‑ACTTTTTTAATGTTACGCGCATTGCAGGGAGGATTGTTCAACGGTCCCACGCTAATGGGGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAggg > 1:31019/1‑250 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTGCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑aa < 2:269690/249‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAACACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACCATTTTT‑TCTCGGGCGCCCATTATGC‑CTT‑A‑CCATTATTTCGTTGGGGGGG‑ACCTTTTTACTGTTCAGCGGATTGAAGGGAGGATTGTTCAACGATACCACACTATTTGTGTGGCCGTTGGTTGGTTTGTtgtgggttgggccagggaa > 2:431196/1‑234 (MQ=255) tACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGtt < 1:231923/250‑1 (MQ=255) aCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑TAACATTTTTTTCTCGGGCGCCCCTTAGGC‑CTA‑A‑CCCTTATTTCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGCATTGCAGGGAGTATTTTTCAACGTTCCCACCCTACTGGTGTGGCTGCTGATTTGTTTGCTGTTTTTTTGCGCAGGT‑AAAGTTct > 1:216959/1‑250 (MQ=255) aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCtt < 1:221099/251‑1 (MQ=255) ttttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCGGGGGCACGTTAGGC‑CTG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTAAGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGGGTGGGTGCTGATTGGTTTGCTGTGTATTGGGGCAGGT‑AAAGTTCGTCTTTATTATCATGCTTAt > 1:150469/1‑251 (MQ=255) tttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTCGGGGGCACGTTAGGG‑CTG‑A‑CCATTATTGCGGTGGGCGTG‑ACTTTTTTACTGTTATGCGCCTTGCAGGGGGGGTTGTTTAACGGTTCCACGCTACTGGTGTGGCTGCCTTTTGGGTTTCTGTGTTTTGGCGCAGGG‑AAAGTTCTTCTTTAttttttttcttttt > 2:370444/1‑239 (MQ=255) gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGGGATGGGCGTG‑ACTTTTTTTATGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCGACTGGTGGGGGTGTTGATTGGGTTTCTGTGTATTGGCGCAGGT‑ACAGTTCGTCTTCATTATCATGCTTATCTGAc > 1:127313/1‑251 (MQ=255) gcgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAg < 2:75299/251‑1 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTTCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGa < 2:38317/251‑1 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTTCGTTGGGCGGG‑ACTTTTTTTCTGTTACGCGCATTGCCGGGAGGATTGTTCAACCTTTCCAAGCTACTGGTGGGGCTGCTGGTTGGTTTGCTGTGTGTTTGCGCAGGGGAAAGT‑CGTCTTCATTATCCTGCTTTTCTGGCAGCTGCTTCACGTAATg > 2:399868/1‑250 (MQ=255) gAGCACCGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCCTTATTGCGATGGGCGTG‑ACTTTTTTACTGTTAAGCGCATTGCAGGGAGGATTTGTCAACGGTACCACGCTACTGGTGGGGCCGCTGGTTTGGTTGCTGTGTATTTGCGCAGGG‑AAAATTCGGCTTCATTATTATGCTTATCTGACAGCTGCTTCACGTAATGATa > 1:122414/1‑250 (MQ=255) cACTGGCAGCTTGATGATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTTTCGGGAGCACGTTAGGC‑ATG‑A‑ACATTATTTCGGTGGGCCTG‑ACTTTTTTTCTGTTACGCGCATTACAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTTGTTTTCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTTACCGCTGCTTCACGAAAAGATAGcc > 1:298248/1‑250 (MQ=255) aCTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTATCTTGGGACCACGTTAGGC‑CTG‑A‑CCCTTTTTTCGAAGGGCGGG‑AATTTTTTAATGTTTAGCGCATTGCCGGGAGTATTTTTTAACGGTCCCCCGCTACTTGTGGGGCTGCTGTTTGGGTTGCTGTGTATTTGAGCCGGT‑AAAGTTCTGCTTCATTATCATGCTTTTCTTACAGCTGCATCACATAAAGATAGCCac > 2:262822/1‑250 (MQ=255) tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGGG‑ACTTTTTTACTGTTAAGCGCATTGCAGGGAGTATTGTTTAAAGTTCCCACGCTACTGGGGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTTTCATGCTTATCTGACAGCTGCTTCCCGTAATGATAGCCCTGc > 1:10941/1‑251 (MQ=255) cAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAAAATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTTGTACGCGCAATGCAGGGAGGGTTGTTCAACGTTCCCACGCTACTGGAGTGGGTGCTGATTTGTTTGCTGTGTATTGGCGCAGGG‑AAAGATCGTTTTCATTATTAAGCGTATCTGAAAGCCGCTTCACGGAATGCTAGCCAAGcccc > 1:181962/1‑250 (MQ=255) aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGTGCGCACGTTAGGC‑ATG‑C‑CCATTATTGCGATGGGCGTG‑ATTTTTTTTCTGGTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGGTTGCTGTGTATTGTCGCAGGT‑AAAGGTCGTCTTCATTATCATGCTTATCTGGCAGCTGCTTCACGTAATGATAGCCATGCCCGt < 1:193266/251‑1 (MQ=255) gATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCAc < 1:370444/251‑1 (MQ=255) gATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTAGAGCGGACGTTAGGC‑ATG‑C‑CCATCTTTGCGGTGGGGGTGGACTTTTT‑ACTAATACGCGCATTGCAGGGAGAATTGTTATACCTTCCCACACCACTGGTGTGGCTGCTGATAGGTTTTCTGTGTATTGGCGCAGGG‑TAAGTTCGTCTTCATTATCTTGCTTATCTGCCAGCTGCTTAACGTAATGATTGCCAATCCCGTGCCAc > 1:53188/1‑251 (MQ=255) tcGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATT‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGG‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGAtgg > 2:330397/1‑251 (MQ=255) cGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTTTCTCGGGGGCACGTTAGGC‑ATG‑A‑CCCTTATTTCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCCCGCCAATGGTGTGGCTGCTGGTTGGTTTGCTGGGTATTTGCGCAGGG‑AAAATTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTTACGGAATGATAGCCATGCCCCTGCCACGAtggc > 1:67152/1‑251 (MQ=255) gAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGTAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTTGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCg < 1:62041/250‑1 (MQ=255) cGTTCTTTCGGCGGGTG‑AAACTTGTTTCTCTCGGGCGCACGTGATGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGCTTCGCGCATTGCAGGTAGTATTGTTCAACGTTCCCACGCTACTTGTGTGGCGGCTGATTGGGTTGCTGTGTATGGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAAGGAGAGCCAGGTCCGTGCCCCGAGGTCTGGCGAACTc < 2:203068/251‑1 (MQ=255) ggTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGGTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACg < 2:122414/251‑1 (MQ=255) | ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCACGTTAGGC‑ATG‑A‑CCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGT‑AAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACG > NC_000913/119149‑119632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |