Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA37.0% 30.8 / 12.2 27intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (16/1);  new base A (0/10);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 1.30e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATT  >  NC_000913/1824814‑1825305
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     
atgCAATGCTTGCACGCCAGTAAAAATCACGTTTTGCCGGTGGTAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCTCTCCCGGCCCGTTCTTTTCATTGTTATCGTGCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTTTTTGAGAAGagaaaagaaa                                                                                                                                                                                                                                                    <  2:397825/251‑1 (MQ=255)
      tGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGCTTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                                                                                                                                              >  2:401675/1‑251 (MQ=255)
            cACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTAGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                                                                                                                                        >  1:218076/1‑251 (MQ=255)
            cACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCAACGGCAGCATTTCGCCCCAGATTCACCGACATTTTACGCATATCAAAGTATTCAATACTCGCTCCCGGTCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCGGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCAGATCCt                                                                                                                                                                                                                                        >  1:307848/1‑251 (MQ=255)
                                       gtgggatcttccaatttccgcccgTTTCCCCGGCGGCATTCCCCCCCAGATTCCCCGATATTTTACTCATATTAAATTTTCCCATCCTCGCTCCCTGCCCTTTTTTTTCATTTTTTTCTTTCATGTACCCT‑TGGTTTTTTATCGTCAC‑CTCGGTTGTTTTCCTTTTGTTTTA‑ATCGGGTTTTTTTT‑ACAACTTTTTGAAAAGAAAAAAAAATCCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                                                                                                                             <  1:258986/229‑1 (MQ=255)
                                          ggAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                                                                                                                           >  1:88885/1‑250 (MQ=255)
                                               gtgcccaTTTCGCGCGTTCCACCGGCCGCCTTTCACCACAGATTCACCGACATTTTACGCATATCACAGTATTCCATACTCGCTCCCGGCCCGCTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGACGTTCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                                                                                                                     >  2:59369/7‑251 (MQ=255)
                                                                ttCACCGGCATCATTTCCCAACAGATCCCCCGATATTTTCCGCATATCAAAGTATTCCATCCTCGCTCCCGGCCCTTTCTTTTCTTTGTTATCGTTCATGTACCCG‑TGGATGGTTATCGGAAA‑CTCGGGTGATTTACGTTGGGGGTG‑ACCGGGTTTTTTTT‑AAACCGGTGGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                                                                                                                     <  1:366729/250‑1 (MQ=255)
                                                                                         ttCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTcagacgttt                                                                                                                                                          >  1:136846/1‑245 (MQ=255)
                                                                                          tCACCGCCCTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGCTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGACACGAAAACCGCCGATCCTGTCCACCGCATTACTGCACGGTAGTGGACAAGACCGGCGGACTTCAGTTTTTT‑GTATGGAAGGTTATTTAGCAGATGc                                                                                                                                                         >  2:296831/1‑251 (MQ=255)
                                                                                               gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTACGTTTTTT‑GGCTGAAAGATTATTTAGCAGTTGCAGGc                                                                                                                                                     >  1:20890/1‑250 (MQ=255)
                                                                                                    tttacgaatataaaattttcccacccTCGCCCCCGGCCGTTTTTTTTCTTTGTTATCTTTAAGGTACCGG‑TGGATGGTTATCGGCAC‑CGGGGGTGATTTACGTTGGGGGTG‑ATCAGGTTTTTTTT‑ACAACTGTTAGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                                                                                                                                <  2:146696/225‑1 (MQ=255)
                                                                                                         gaatttaaaaGTATCCCATACTCCCTCCCGGCCTTTTCTTTTTTTTGTTATCTTTCGGGTACCGG‑TTGATGTTTTTCGGCAG‑CTGGGGTTATTTACTTGAGTGGTG‑ATCAGTTTTTTTTT‑ACACATGTTGGAGAAAAGAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                                                                                                                          <  1:410676/244‑1 (MQ=255)
                                                                                                            tatCAAAGGATTCCCTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑CGGATGGCTATCGGAAA‑CTCGGGTGCTTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGCGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACAGGCGGTATTTAGTTTTTTTGCCGGGAAG‑TTATTCAGCAGTTGCACGCCTTTTACCtttttt                                                                                                                                       >  2:399997/1‑249 (MQ=255)
                                                                                                                 aatcttcccacccccgcccccGGCCCTTTTTTTTCATTTTTATCGTTCAGGTACCCG‑TGGATGTTGTTCGGCAC‑CTCGGGTGTTTTACGTTGGTGGTG‑TCCAGTTTTTTTTT‑ACAATTTTGTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAAATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                                                                                                                                  <  2:181086/233‑1 (MQ=255)
                                                                                                                             cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTT‑GGCTGAAAGATTATTTCGCAGTTCCAGGGATTTTTCCTTTTGCCGCttggcggtctgttt                                                                                                                      >  2:288745/1‑238 (MQ=255)
                                                                                                                                gCTCCCGGCCCTTTTTTTTTTTTGTTATCGTTCAG‑TGACCGGTGGATGGTTATCTGAAC‑CTCGGGTGATTGACGTTAGGGGGG‑ATAAGGTTTGTTTC‑ACATCTGTTGGAGAAGAAAAAAAAAATCCGCCGATCCTGGCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAATATTATTCAGCAGTTGCATGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                                                                                                                   <  1:262689/251‑1 (MQ=255)
                                                                                                                                   cccGGCCTGTTTTTTTTTTTGTTATCTTTCAGTAAACCG‑TGGATGGTTATCG‑CAATCGGGGGTGATTTA‑GGTGGTGGGGGAT‑AGGTTTTTTTTCACAATTGTTTGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt                                                                                                                <  2:428065/251‑1 (MQ=255)
                                                                                                                                       gCCCGTTCTTTTCATTGTTATCGTTCAGGTACCCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGCGGTG‑ATCAGGCTTTTTTT‑AAAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTT‑GGCTGAAAGATTATTCAGCCGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTTCTGTCAGACGCTtctc                                                                                                            >  1:426187/1‑251 (MQ=255)
                                                                                                                                                        ttATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGCTTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTT‑GGCAGAAAGATTATTAAGCAGTTGGCGGCATTTTAACTTTTGCCGCTGGACGCTCTGTCAGACGCTTCTCAAAATTTGCATTAAAtt                                                                                           >  1:19408/1‑251 (MQ=255)
                                                                                                                                                                               tgtttATCGACAA‑CGCGGGTTTTTTATTTTAGTGGTT‑ATCATGTTTTTTCT‑CCAATTTTTTAAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTTCAGGCATTTTACCTTTTGCCGCTTGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCTGCGTCa                                                                      <  2:131801/247‑1 (MQ=255)
                                                                                                                                                                                  ttATCGGCAA‑CTCGGGTGATTTACGTTAGTGGAG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGCTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCCGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCCTTAAATTGCTTTTTATGTTCCGGCGTCAGGAt                                                                  >  1:399566/1‑251 (MQ=255)
                                                                                                                                                                                                     aTTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGTCAAGACCGGCGGTCTTAAGTTTTTT‑TGCTGAAAGATTATTCAGCAGGTTCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTTCGGCGTCAGGATGTTGTAAATTTTGTTCtt                                                >  2:315563/1‑250 (MQ=255)
                                                                                                                                                                                                                gtggtgttctgtttttttta‑ACACCTGTTTGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtg                                      <  1:381317/238‑1 (MQ=255)
                                                                                                                                                                                                                ggtgttatcagttgtttttg‑ACAACTTTTTGAGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTTGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtg                                      <  2:19309/237‑1 (MQ=255)
                                                                                                                                                                                                                   gtgagctggtttttttt‑ACAATTGTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTgc                                   <  1:359231/241‑1 (MQ=255)
                                                                                                                                                                                                                      aTCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGCAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCCGTTGCAGGCATTTTACCTTTTTCCGCTGGCCGTTCTGTAAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTTCGGCGTCAGGCTGTTGTAAATTTTGTTCTGGGTTTCCATTTGCGcc                                >  2:141355/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTTCTTTTTCTGTTCCGGCGTCAGGATGTTGGAAATTTTGTTCTGGGGTTCCCTGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTTcc     >  2:334566/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    aatagaagaaacCCGCCGTTCCTGTTCATCGTATTACTTCTTGGTAGTGGACACGTACGTCGGTCTTAAGTTTTTT‑TGCTGAAAGATTATTCAGCATTTGTAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAGTAGCAGTAAGTTGCGTTTTCTGTTCTGTCGTCAGGATGTTGTAAATTTTTGTCTTGGTTTCCATGTGCGCCAGCATTTTAGCATTGCGTGGGTCTTCCAt   <  2:185384/246‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaaaaaaaaatCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCAtt  <  2:29177/249‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     
ATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCG‑TGGATGGTTATCGGCAA‑CTCGGGTGATTTACGTTAGTGGTG‑ATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTT‑GGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATT  >  NC_000913/1824814‑1825305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑