Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,117,984	IS30 (+) +2 bp	coding (106‑107/846 nt)	glpF ←	glycerol facilitator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1469296 =	NA (NA)	35 (0.820)	30/492	0.4	97.2%	noncoding (1/1221 nt)	IS30	repeat region
	?	NC_000913	= 4117985	1 (0.020)					coding (106/846 nt)	glpF	glycerol facilitator
*	?	NC_000913	4117984 =	0 (0.000)	43 (1.020)	41/488	0.2	100%	coding (107/846 nt)	glpF	glycerol facilitator
	?	NC_000913	4507459 =	NA (NA)					noncoding (1221/1221 nt)	IS30	repeat region

ACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTG  >  NC_000913/1469046‑1469540                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      aCGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAAGTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACtt                                                                                                                                                                                                                                                       <  2:161412/251‑1 (MQ=255)       gagaCCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAt                                                                                                                                                                                                                                                 <  1:224682/251‑1 (MQ=255)                                                        ccTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGt                                                                                                                                                                                                <  1:113355/250‑1 (MQ=255)                                                                   cgacGTCGGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTACCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATgtg                                                                                                                                                                                    <  2:141999/248‑3 (MQ=255)                                                                     ccGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGAc                                                                                                                                                                                  >  1:33948/1‑251 (MQ=255)                                                                             gattCTCCGGCACTAAGACATCAATCATCTGTTCTCCAATGACTATTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGCGATTCAGGTGTCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTCCTTGTAGATTCAATTGGTCAACGCAACCGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAAACGAACAttt                                                                                                                                                                          >  2:248894/3‑251 (MQ=25)                                                                                    cGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGCCTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGACCATTTAcagc                                                                                                                                                                     >  2:195831/1‑249 (MQ=255)                                                                                     ggCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagc                                                                                                                                                                     >  2:306283/1‑248 (MQ=255)                                                                                      gCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                                                                                                                                   >  1:21535/1‑249 (MQ=255)                                                                                                       atcTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGtttt                                                                                                                                                >  2:312280/1‑251 (MQ=255)                                                                                                                 cAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATgg                                                                                                                                      <  1:84718/251‑1 (MQ=255)                                                                                                                              aaaaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACa                                                                                                                          <  1:29817/250‑1 (MQ=255)                                                                                                                                      gTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGt                                                                                                                 <  1:306283/251‑1 (MQ=255)                                                                                                                                                    aCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGCTGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTAAAGCAGAGGAAAAGGCCTCTGTTTTTGAACTATGGAAGAACGGCACAGGCTTCAGTGAAATAGCGAatat                                                                                                   >  1:183867/1‑251 (MQ=255)                                                                                                                                                          ttAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTgg                                                                                              <  1:42565/250‑1 (MQ=255)                                                                                                                                                          ttAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCGTCAGTGAAATAGCGAATATCCTgg                                                                                              <  2:379492/250‑1 (MQ=255)                                                                                                                                                                gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCaa                                                                                        >  1:286929/1‑250 (MQ=255)                                                                                                                                                                    ttGGTTGTCTGGAGATTCAGGGCGCCAGTCTAAACACCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATCAAGGTACTTGTAGCTTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAccc                                                                                   >  2:41861/1‑251 (MQ=255)                                                                                                                                                                     tGGTTGTCTGGAGATCCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGAACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCg                                                                                  >  1:163813/1‑251 (MQ=255)                                                                                                                                                                          gTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACg                                                                             >  2:154885/1‑251 (MQ=255)                                                                                                                                                                                 gaTTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCa                                                                      <  1:406336/251‑1 (MQ=255)                                                                                                                                                                                                              cttacttaTGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATaaa                                          >  2:188102/1‑250 (MQ=255)                                                                                                                                                                                                                ttcttaTGCTGACTCCGGTGTGAAAATTGTTAATAAAGGGTCTTGTAGACTCAATTGGGCAACGCAACAGTTATGTGAAAACATGGGGGTTG‑GGAGGTTTTTGGAACGAGGCGAACATGTACAGCAGGGGAAAAAGCCTCTGTTTTTGAAGTGTGGAAGAACGGAACAGGCTTCAGTGTAATGTCGAATTTCCTGGGTTCAAAACCCGGAACGAGCTTTACTATGTTAAGGGATACTGGCGGCATaaaa                                         <  1:404488/247‑1 (MQ=14)                                                                                                                                                                                                                         tGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAggg                                                          <  1:53379/223‑1 (MQ=255)                                                                                                                                                                                                                         tGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAggg                                                          >  2:53379/1‑223 (MQ=255)                                                                                                                                                                                                                                       aaaTTGTTAATAAAGGTAATTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGCGACGAACATTTACAGAAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGCACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACACGGCACGATCTTCACTATGTTAAGGGATACTGGCGACATAACACCCAATGAGCGTAAGCGGGCTGt                  >  2:184033/1‑249 (MQ=11)                                                                                                                                                                                                                                                     ggTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTg  >  2:342815/1‑251 (MQ=32)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      ACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGG‑TTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTG  >  NC_000913/1469046‑1469540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.