Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,429,986	IS3 (–) +5 bp :: +T	coding (34‑38/258 nt)	yrdB ←	DUF1488 domain‑containing protein YrdB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	39 (0.920) +T	35/490	0.3	97.5%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3429986 =	1 (0.020)					coding (38/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB
*	?	NC_000913	1094245 =	NA (NA)	49 (1.160)	47/490	0.1	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3429990	0 (0.000)					coding (34/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB

ACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAACG  >  NC_000913/314983‑315475                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         cattcatccaggcatttattgctatgacatgttctatcagaaaggaaaaactccttttctggcagggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttcgctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCtt                                                                                                                                                                                                                                                       >  2:332814‑M1/247‑250 (MQ=255) cattcatccaggcatttattgctatgacatgttctatcagaaaggaaaaactcctgttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctcgggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaagtgtccgcgtgaatcagtccttccagtttccggacagggaagagtgaTCtt                                                                                                                                                                                                                                                       >  1:398963‑M1/247‑250 (MQ=255)           aaTGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCAATTTCTGCCGAGGTGTCCTATACGCCAGTGACTCTTAACCAGCAAt                                                                                                                                                                                                                                             >  2:319799/1‑250 (MQ=255)                  atatTCGGGCGTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGaca                                                                                                                                                                                                                                     <  2:379861/251‑1 (MQ=255)                    tgctatgacatgttctatcagaaaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggcagagtgaTCTTACCCAGCAATAGTGGACAcg                                                                                                                                                                                                                                   <  1:317989‑M1/24‑1 (MQ=255)                    tgctatgacatgttctatcagaaaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGacac                                                                                                                                                                                                                                    >  1:141422‑M1/228‑250 (MQ=255)                     gctatgacatgttctatcagaaaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACAcg                                                                                                                                                                                                                                   >  2:292088‑M1/227‑250 (MQ=255)                      ctatgacatgttctatcagaaaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACAcgcg                                                                                                                                                                                                                                 <  1:359888‑M1/26‑1 (MQ=255)                        atgacatgttctctcagaaaggaaaaactccttttctggcatggtgtgagcagcgcggctcaaagcgtaatgctgatggtttaggcatgctggcggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgccgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaggagtgaTCTTACCCAGCAATCGTGGACACGCGGc                                                                                                                                                                                                                               <  2:71589‑M1/28‑1 (MQ=255)                        atgacatgttctatcagaaaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacaggtaagagtgaTCTTACCCAGCAATAGTGGACAcgcg                                                                                                                                                                                                                                 >  1:393342‑M1/224‑249 (MQ=255)                         ggcatCTGGCTGGAAAAAATCGCCGGAGATTGAGGATTATCAGGCACGCCCGTCGAATGGCTGGGATTTTCGTGCTGAGGCATTTTTCCCCGCCGGGCCGCAGCTTGGCTCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTTGGGCTGGTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGATGTGACCTATACGCCAGTTCCTCTTACCCAGCAATAGTGGACCCGCGGCt                                                                                                                                                                                                                              <  1:366915/247‑1 (MQ=255)                          gCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTa                                                                                                                                                                                                                             <  1:407302/251‑1 (MQ=255)                             tCTGGCTGGAAAAAAGCGCCGGATATGGAGGATTATCAGGAACGCCCGGCGAATGGCGGGGATTTCCGTGCTGAGGGCTATTTCCCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTaa                                                                                                                                                                                                                            <  2:68685/249‑1 (MQ=255)                              ctggctggAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTg                                                                                                                                                                                                                         >  2:130396/1‑251 (MQ=255)                                          aaggaaaaactccttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACtctc                                                                                                                                                                                                              <  2:80026‑M1/45‑1 (MQ=255)                                               ccGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagtc                                                                                                                                                                                                          >  2:121432/1‑249 (MQ=255)                                                       ttttctggcatggtgtgagcagcgaggctcaacgcgtaatgctgatggtttaggaatcctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACt                                                                                                                                                                                               <  1:429697‑M1/59‑1 (MQ=255)                                                       ttttctggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACt                                                                                                                                                                                               <  1:174359‑M1/59‑1 (MQ=255)                                                          tctggcatggtgtgagcagcgagtctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgcatccggccatccagtttccggacagggaagagtggTCTTACCCCGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGTG‑GTGACTcac                                                                                                                                                                                            <  2:299741‑M1/62‑1 (MQ=255)                                                             ggcatggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATg                                                                                                                                                                                         >  1:230562‑M1/187‑251 (MQ=255)                                                               catggtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTCAACTCTCAGTCCGAG‑GTGACTCACCTGAc                                                                                                                                                                                       <  2:286163‑M1/67‑1 (MQ=255)                                                                 cAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCAGGCTATGTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATGGGGGACTCGTGTCTAAGTGAGGAAACTCTCAGTCAGAG‑GTGACTCACATGACa                                                                                                                                                                                      <  1:388817/250‑1 (MQ=255)                                                                   gtgtgagcagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccaggttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACaaaa                                                                                                                                                                                   >  1:31375‑M1/181‑251 (MQ=255)                                                                     aaCGCCCAGCGAATGGCTTGGATCTTCGTGCCGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCCAGCCTGATGTATGAACAGTATTATGGCGATGGCGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACCAGCGGATAAGTGAGTAAACTCTCATGCCGAGCGT‑ACTCACATGACaaaaa                                                                                                                                                                                  >  2:316391/1‑250 (MQ=255)                                                                       cGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGCTAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGCG‑GTGCCTCACATGCCAAAAAc                                                                                                                                                                                 >  2:368011/1‑249 (MQ=255)                                                                          ccGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTAt                                                                                                                                                                            >  1:350721/1‑251 (MQ=255)                                                                          cagcgaggctcaaagcgtaatgctgatggtttaggaatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTAt                                                                                                                                                                            <  2:90024‑M1/78‑1 (MQ=255)                                                                                       ggaatatTAGTGCTGAGGTCTATTTACCCGCCTGGCCTCCGCTTGGCGCTAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTTTTTGGTAAAGATAAGCGCCAGAAAGACCCTCATGCTATTTCTGCCTAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCTGCTAAGTGAGTAAACTCTTAGGCAGAT‑GTTACGCACTTGACAAAAACAGTATCAA‑CCAGTaaaaa                                                                                                                                                              <  1:178095/248‑1 (MQ=255)                                                                                               cGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGGTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCGAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTa                                                                                                                                                      <  2:393133/251‑1 (MQ=255)                                                                                                             aatgctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgtatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCt                                                                                                                                        >  2:291133‑M1/139‑251 (MQ=255)                                                                                                               agctggaggcacagacggctcatgcctttcttctctggcactgtgttctgcctgactaagagccagttataaagcaattgcaggaggaattgtccgcgtgcatcatgccatccagtttacggacagggaagagtgaTCTTAACCAGCAATAGTGGACACGCGACTAAGCGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGCCACAAACAGTATCAA‑CAAGTAAAAAACCCCGAAAACAgcattcccatg                                                                                                                                       <  2:382939‑M1/114‑6 (MQ=255)                                                                                                                gctggtggcacaggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggatgaattgtccgcgtgaatcaggccatccaggttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAGCAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGa                                                                                                                                      >  2:366340‑M1/136‑250 (MQ=255)                                                                                                                           aggcggctcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTg                                                                                                                           <  2:307465‑M1/126‑1 (MQ=255)                                                                                                                            ggcggatcatgcctttcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGa                                                                                                                          <  1:92350‑M1/127‑1 (MQ=255)                                                                                                                              cggctcatgcatttcttctctggaacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagcgtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTCAAAAACCCCGAAAACAGCATTCGCCTGAATTTCGCAGTGAAGc                                                                                                                       <  2:161522‑M1/130‑1 (MQ=255)                                                                                                                               gCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACCTGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAAGGAAGcc                                                                                                                      >  2:266068/1‑251 (MQ=255)                                                                                                                                           tcttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggcggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCCTTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCt                                                                                                          <  2:241375‑M1/143‑1 (MQ=255)                                                                                                                                             ttctctggcacggtgttctgcctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTg                                                                                                         <  1:115552‑M1/144‑1 (MQ=255)                                                                                                                                                        cAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTa                                                                                                                                                      <  2:322931/194‑1 (MQ=255)                                                                                                                                                        cAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTa                                                                                                                                                      >  1:322931/1‑194 (MQ=255)                                                                                                                                                               atGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GAGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGACGCTTGCTGAACGCATCGGTGTTACTGc                                                                                      >  2:125018/1‑251 (MQ=255)                                                                                                                                                                 cctgacgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGCCACGCGGCTAAGTGAGTACACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCCTCGGTGTTACTGCCg                                                                                    <  2:248887‑M1/165‑1 (MQ=255)                                                                                                                                                                      cgtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGcc                                                                               <  1:224554‑M1/170‑1 (MQ=255)                                                                                                                                                                       gtagaaccagttataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccgggcagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAACACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGCATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGccc                                                                              <  2:97707‑M1/171‑1 (MQ=255)                                                                                                                                                                         aGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGt                                                                            <  1:89547/251‑1 (MQ=255)                                                                                                                                                                          ggcGGGCTGGTTGGTAAAGAAAAGCGCCAGAAAGACCCGCATGCTATTTCAGCCGAGGTGACCTATACGCCAGGGCCTCTTACCCAGCAATAGGGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GGGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCAGAATCAGCATTCGCCTGAATTGCGCGGTGAAGCCCTGAATCGTGCTGAACGCATCGGTGTTTCTGCCGCGGCCCGTg                                                                           <  2:418460/249‑1 (MQ=18)                                                                                                                                                                                   ataaagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGcc                                                                  <  1:243661‑M1/183‑1 (MQ=255)                                                                                                                                                                                      aagcaattgcaggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAAATCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGt                                                               <  1:174201‑M1/186‑1 (MQ=255)                                                                                                                                                                                              tAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAATGGCCACGCGGCTAAGTGAGTAAACTCTCCGTAAGAG‑GGGACTCACATAACAAAAACAGAATCAAACCA‑TAAAAAACCCCGTAAACAGAATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATc                                                        >  2:348458/1‑250 (MQ=17)                                                                                                                                                                                                aggaggaattgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                                      <  2:112144‑M1/195‑1 (MQ=255)                                                                                                                                                                                                     cataaaGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGTCTAAGTGAGTAAACTCTCAGTCAGAG‑GTTACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATACGCCTGAACTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAAc                                                   <  1:2996/245‑1 (MQ=255)                                                                                                                                                                                                     cAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCAAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTAACCTGACAAAAACAGTATAAA‑CCAGTAAAAACCCCCGTAAACAGCATTCGCATGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCAAGTGAACTCAGGCTGTGTGACTaacaac                                                   >  2:2996/1‑248 (MQ=17)                                                                                                                                                                                                       attgtccgcgtgaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTAc                                              <  2:333512‑M1/203‑1 (MQ=255)                                                                                                                                                                                                        aaaGACCCGCATGCTATTTCTGCCGAGGTGCCATATACGCCCGTGCCTCTTACCCAGCAATAGTGGACACGCGCCTAAGTGAGTAAACTCCCAGTCACCG‑GAGACTCACATGACAAAAACAGCATCAA‑CCCGGCAAAAACCCCGCAAACAGCAATCGCCTGAATTTCGCAGTGAAGCCCTGACGCTTGCTGAACGCATCGATGATCCTACCGCAGCCAGTACACTCAGCCTGCATGAATCACAActcgac                                              >  2:413359/1‑247 (MQ=255)                                                                                                                                                                                                            ccgcgtgaagcaggccatgcagtttcctgacagggaagagtgaTCTTACCCAGCAATAGTGGTCACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACCCAGCCTGTATGAATCATAACTCTACAACt                                          >  2:95719‑M1/44‑250 (MQ=255)                                                                                                                                                                                                              ccGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGgc                                       <  1:231224/251‑1 (MQ=255)                                                                                                                                                                                                                gCATGCTATTTCTGCAGAGGTGACCTATACGACAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CAAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGACTGTATGAATCACACCTCTACAACTGgcgc                                     >  1:354657/1‑251 (MQ=255)                                                                                                                                                                                                                  gaatcaggccatccagtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAg                                   >  2:84080‑M1/38‑251 (MQ=255)                                                                                                                                                                                                                    atcaggccatccaggttccggacagggaagattgaGCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACATTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATACGCCGGAATTTCGCAGTGAAGCTCTGAAGCTTGCTGAACGCATCGTTGTGACTGCCGCAGTccgtgaacgcagcctggatgaatcgcatcccgacaacgggctcagta                                 >  2:174760‑M1/36‑228 (MQ=255)                                                                                                                                                                                                                             gCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTGGCTTAACGCATCTGTGTTACTGCCGCAGCCCGTGAGCTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTATACAGCa                          <  1:379009/249‑1 (MQ=255)                                                                                                                                                                                                                                agtttccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATc                     >  2:115552‑M1/24‑251 (MQ=255)                                                                                                                                                                                                                                     ccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCa                                    >  1:32383‑M1/19‑231 (MQ=255)                                                                                                                                                                                                                                     ccggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCa                                    <  2:32383‑M1/213‑1 (MQ=255)                                                                                                                                                                                                                                      aCCTATACGCCAGTGCCTCTTCCCCAGCAATAGTGGCCACGAGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGCACTCAGCCTGTATGAATCACAACTCTAAAACTGGCGCAGTAAACAGCAAAATcagc                  >  2:416417/1‑248 (MQ=255)                                                                                                                                                                                                                                       ggacagggaagagtgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAc              <  1:77521‑M1/235‑1 (MQ=255)                                                                                                                                                                                                                                             cGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGtcttc        <  1:130396/251‑1 (MQ=33)                                                                                                                                                                                                                                                   gtgaTCTTACCCAGCAATAGAGGAAACGCGGCTCAGCGAGTAAAATCTCAGTCAGAG‑AAGACCCACATGACAAAAACAGTATCAA‑ACAGTAAAAAACCCCGTAAACAGCATTCGCATGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAACAAAATCAGAAGACGACttctgcacg  <  2:75624‑M1/247‑5 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         ACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAG‑GTGACTCACATGACAAAAACAGTATCAA‑CCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGAACG  >  NC_000913/314983‑315475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.