| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,259,415 | +G | intergenic (‑119/+304) | psuK ← / ← fruA | putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,259,415 | 1 | . | G | 87.5% | 19.9 / ‑1.7 | 8 | intergenic (‑119/+304) | psuK/fruA | putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (5/2); total (5/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
CTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACG‑T‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATA > NC_000913/2259170‑2259633 | cTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACggtaa > 1:15160/1‑246 (MQ=255) ttttaCCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCAGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCaa > 1:182904/1‑250 (MQ=255) cGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGGCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACG‑GGAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAgg < 2:235726/251‑1 (MQ=255) aTCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAg < 1:245378/251‑1 (MQ=255) aaTTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATg > 2:160206/1‑251 (MQ=255) tACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTAATATTTAAACATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGAAGGCTCACAAAATGCGATTAAATACCTGAATTACAAAAGCGAGGCATTTGTGACGAGATGcac > 1:35406/1‑251 (MQ=255) aGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGc > 1:101336/1‑250 (MQ=255) ggCAAAGACGGTTTAAACATGTTCTCATATTTAAACAGGTGCTCATAGTAGGCAGTTTTTACGGT‑AAAGTAAAGTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGTATGCACAGAACAGAACAAGAATAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGATGGTTTGATGAActtgggaagttt < 2:257584/250‑12 (MQ=255) aaaCATGTGCTCATAGTATGCATGTTTTACGGT‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa > 1:67323/1‑250 (MQ=255) | CTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACG‑T‑AAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATA > NC_000913/2259170‑2259633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |