Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,5490G.96.8% 263.0 / 1.1 63coding (179/606 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base . (29/31);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207311‑3207797
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        
cACGCTGCTGGTTCATGACGATCTCACCTTTGCTAATCTGTCTCAAACCCGGAGCGCGCTATGAAAAAGATCCTGACAACCCAGCTCAAAGCTGAA‑GATCTGCAAGCTATTCGCGTCGACGATGTGATCTACCTGACCGGTACGCTGGTGACTTGCCGCGACGT‑TTGTCCCCGCCGTTTGATCGAACTGAAAAGTCCGATTCCTTACGATCTCAACGGCAAAGCGATTTTCCAAGCTG‑CCCCAtagttcgc                                                                                                                                                                                                                                             >  2:65791/1‑244 (MQ=255)
   gttgctgttgcatcccGATCTCACTTTTGAAAATCTGTCTCCCCCCCGGAGCGCTTTATGAAAAAGATCCGGACAACCCAGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGCCCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaa                                                                                                                                                                                                                                          <  2:129027/237‑1 (MQ=255)
      gctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAAc                                                                                                                                                                                                                                       >  1:93102/1‑251 (MQ=255)
        tgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAACGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                     >  2:424025/1‑251 (MQ=255)
            ccactccgttctcACCTTTGAAAATCTGTCTCCCACCCGGTGGGTTTTATGAAAAAGATCATGCCAACCCCGAT‑AAAGGTTAACGAGCTGCGATATATTGGCGTCGGCGTTGTTATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGCCGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCGTGCGCAAAAACGGCGAc                                                                                                                                                                                                                                 <  2:107914/242‑1 (MQ=255)
                   gATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                          >  1:171280/1‑251 (MQ=255)
                   gATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                          >  2:420196/1‑251 (MQ=255)
                               gAAAATCTGTCTCACACCAGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGAGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTAACCGCCGTTTGCTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGACTCCg                                                                                                                                                                                                              >  2:323198/1‑251 (MQ=255)
                                   aTCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCgg                                                                                                                                                                                                          >  1:98231/1‑251 (MQ=255)
                                       gcctcACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGcc                                                                                                                                                                                                        <  2:26483/247‑1 (MQ=255)
                                       gTCTCACACCCGGAGCGCGTTATGAAAAAGAGCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTTCCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGccc                                                                                                                                                                                                       <  1:193152/250‑1 (MQ=255)
                                         ctcACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCgcc                                                                                                                                                                                                    >  2:114474/1‑249 (MQ=255)
                                             cacCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                                >  2:265817/1‑251 (MQ=255)
                                               ccgggcgcgattgaTGAAAAAGACCCTGACAACCCCGATCAAAGCGGAA‑GAGCGGCAAGATATTCGCGGCGGCGATGGGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                              <  2:134787/238‑1 (MQ=255)
                                                ccGGAGCGCGTTATGAAAAAGCTCCTGACAACCCCTCTCAAAGCTGAA‑GACCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGTCCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACCGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGt                                                                                                                                                                                             <  2:135909/251‑1 (MQ=255)
                                                     gcccgtaaggAAAAAGACCCTGACAACCCCGATCAAAGCGAAA‑GTGCTGCAAGATTTTCGCTAGGGCGAGGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCGCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAGGGTCTCCGTCGTCCCGACAACCAGTATGCg                                                                                                                                                                                        <  2:288511/242‑1 (MQ=255)
                                                         gTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GTTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACG‑TTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGGGATGGTCTCCGTCGGCCCGACAACCTGGATGCGAATg                                                                                                                                                                                    >  2:83677/1‑251 (MQ=255)
                                                                 aaagaccttGACAACCCCGATCAAAGCTGAA‑GAGCTGCAATATATTCGCGTCGGCGCTGTGATCTACCTGACCGCTACGCCGTTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGttt                                                                                                                                                                            <  1:54298/243‑1 (MQ=255)
                                                                 aaaGATCCAGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGAACTGCCGCGACGT‑TTGTAACCGCCGTTTGATCGGACTGAAACGTCCGATCCCTTACGATCTCAACGGCACAGCGATTTTCCAAGC‑AGCCCCATCGTGCGCAACAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCAGACAACCAGTATGCGAATGGCAAGttt                                                                                                                                                                            >  2:381076/1‑251 (MQ=255)
                                                                   agacccggaaacccccGATCAACGCGGAA‑GATCTGCAAGATATCCGCTTCGGCGGTGTGATCTCCCTGACCGTTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTTAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATTGGAGATGGTCTCCGTCGGCGCGACAACCAGTATGCGTATGGAAAGTTTTg                                                                                                                                                                          <  2:372916/239‑1 (MQ=255)
                                                                     aTCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGa                                                                                                                                                                         >  1:81303/1‑250 (MQ=255)
                                                                          gACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCGGGTGACCTGCCGCGCCGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGTCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGt                                                                                                                                                                     <  1:294311/249‑1 (MQ=255)
                                                                             aaCCCCGATAAAAGCTGAA‑GATCTGAAAGGTATTCGCGTCGGCGAGGTGATCTACCTGCCGGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGTACGTGAAtt                                                                                                                                                                <  2:203284/251‑1 (MQ=255)
                                                                               ccccGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTa                                                                                                                                                              <  1:321811/251‑1 (MQ=255)
                                                                                    aTCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCAATCGTGCGCAAAAACAGCGACAAATGGGAGATGGACTCAGTCGGCACGACAACCAGTATACGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                           >  1:17412/1‑249 (MQ=255)
                                                                                     acAAAGCTGAA‑GATCTGCAAGATATGCGAGTCGACGTTGTGACCTACCGGCCCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCAACGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCTACAAAGGGGATATGGTCTCCGTCGGCCCGACAACCAGTAGGCGTATGGAAAGTTTTGAACGGGAATTTATTGAGc                                                                                                                                                        <  2:210717/250‑1 (MQ=255)
                                                                                          gCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAcc                                                                                                                                                   >  2:393011/1‑251 (MQ=255)
                                                                                          gCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAcc                                                                                                                                                   >  1:341698/1‑251 (MQ=255)
                                                                                             aaa‑GATCAGCAAGATATTCGCGTCGGCGATGGGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGc                                                                                                                                                <  1:100320/250‑1 (MQ=255)
                                                                                                    cTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                             >  2:312781/1‑248 (MQ=255)
                                                                                                         aGATATTCGCGTCGGCGTTGTGATCTACCGTACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggt                                                                                                                                     <  1:31128/251‑1 (MQ=255)
                                                                                                          gATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggtg                                                                                                                                    >  1:399201/1‑251 (MQ=255)
                                                                                                              ttCGCGTCGGCGATATGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAAGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTACACGCTG‑CCCCATCGTGCGCAAAACCGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAACGTTTTGAACGTGAATTTACTGAGCAGACCGGAGTGAAACTGGTGGTTg                                                                                                                                >  1:289206/1‑251 (MQ=255)
                                                                                                                  cgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCa                                                                                                                             >  1:424215/1‑250 (MQ=255)
                                                                                                                      agagataagATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGATTGATCGCACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAACTGGGAGACGGTCTCCGTCGGACCGACAACCAGTATGCGTATGGAAGGGTTTGAACGTGAATTTATTGCGCAGACCGGCGTGAAACTGGTGGTTGGCACAggt                                                                                                                        >  2:250610/9‑251 (MQ=255)
                                                                                                                            gtgATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATg                                                                                                                  >  1:248779/1‑251 (MQ=255)
                                                                                                                                 cTACCTGACCGGAAAGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCGAAGGTGGTATGGGGcc                                                                                                             >  2:407995/1‑251 (MQ=255)
                                                                                                                                   gccggcccGGGACGCGGGTGACCTGCCGC‑TGGTGTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGTAAAGTTTTGAACGTGAATTTAGTGAGCAGACCGGCGTGAAACTGGTGGGTGGCAAAGGTGGTATGGGGcc                                                                                                             <  2:289912/243‑1 (MQ=255)
                                                                                                                                      gacccGGTCCGCGGGTGCCCGGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATGTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTGTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTTGTTTGCAAAGGTGGTATGGGGCCGCTGa                                                                                                        <  2:215117/248‑1 (MQ=255)
                                                                                                                                        cccGGTCCGCTGGTGACTGGCCGCGACGT‑TTGTCCCCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                                      <  1:320167/250‑1 (MQ=255)
                                                                                                                                        cccGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                                      <  1:6405/250‑1 (MQ=255)
                                                                                                                                            gTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCGCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAAGGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTTAATTTATTGAGCAGACCGGCGTGAATCTGGTTGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaag                                                                                                  <  1:39352/251‑1 (MQ=255)
                                                                                                                                                   ggTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGGATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCt                                                                                            <  1:52929/250‑1 (MQ=255)
                                                                                                                                                     ggACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                         <  2:341698/250‑1 (MQ=255)
                                                                                                                                                                gACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCa                                                                                >  1:184188/1‑249 (MQ=255)
                                                                                                                                                                  ccgttggtgtccgccgTTGGATCGAACTGAAACGTCCGATCCCTTACGAGCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAgg                                                                             <  2:191919/241‑1 (MQ=255)
                                                                                                                                                                        gTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTAAAGGCGCTAc                                                                       >  1:62088/1‑251 (MQ=255)
                                                                                                                                                                           cccgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtg                                                                    <  2:98231/250‑1 (MQ=255)
                                                                                                                                                                                 gTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAttt                                                              >  1:68945/1‑251 (MQ=255)
                                                                                                                                                                                     gttCGAACTGAACCGTCCGATCCCTGACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATTGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCTTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTcct                                                          <  2:234091/249‑2 (MQ=255)
                                                                                                                                                                                          aaCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGc                                                       >  1:360750/1‑249 (MQ=255)
                                                                                                                                                                                                                cGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAc                               >  1:144365/1‑251 (MQ=255)
                                                                                                                                                                                                                    ctcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAccc                             >  2:296931/1‑249 (MQ=255)
                                                                                                                                                                                                                      cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAc                               <  1:4178/245‑1 (MQ=255)
                                                                                                                                                                                                                      cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGt                         <  1:345473/251‑1 (MQ=255)
                                                                                                                                                                                                                        aCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg                       <  1:149782/251‑1 (MQ=255)
                                                                                                                                                                                                                          ggCAAAGCGATTTTCCGCGC‑GTCTCCATCGTGCGCAAAAACGGCGACAAATTGGAGATGGGCTCCGTTGGCGCGACAACCAGTAGGCGTATGGAAAGTTGTGAACGTGAATTTATTGAGCAGACCGGCGTTAAACTGGTGGTTGGCAAAGGTGGTATGGGGCGGCTGTCCGAAGAAGGCTGCCAGAAATTCAAGGCGCTTCATGTGATTTTCCCGGCAGGCTGCTCTGTGCTGGGGGCAACCCATGTGGaa                     <  2:372327/251‑1 (MQ=255)
                                                                                                                                                                                                                          ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGaa                     <  2:424215/251‑1 (MQ=255)
                                                                                                                                                                                                                                gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACGAATGGGAGATGGTCTGCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACTGGAATTTATTGAGCAGACCGTCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGTGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTGTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGAtt               <  2:148254/251‑1 (MQ=255)
                                                                                                                                                                                                                                gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGCCAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGAtt               >  1:216630/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTgaa            <  1:142202/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTg      >  2:299193/1‑251 (MQ=255)
                                                                                                                                                                                                                                          aCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGATGGAAGAGATTGAAGAAGTGc     >  1:325780/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGTCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACt  <  1:65791/249‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        
CACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAA‑GATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGT‑TTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207311‑3207797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑