| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,125,694 | Δ1 bp | coding (133/585 nt) | rimJ → | ribosomal‑protein‑S5‑alanine N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,125,694 | 0 | T | . | 95.0% | 76.8 / ‑2.0 | 20 | coding (133/585 nt) | rimJ | ribosomal‑protein‑S5‑alanine N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (11/8); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
AAATGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCT > NC_000913/1125468‑1125935 | aaaTGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGcc > 2:231467/1‑250 (MQ=255) aTTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCTACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCATTGCGCGACGAAAGCCACTGTTAt < 1:311952/251‑1 (MQ=255) ataGTAATACTTACAGCGGATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTaa < 2:149490/251‑1 (MQ=255) aaaGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTc < 1:82233/250‑1 (MQ=255) aCGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTTGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACTAATTTCATAAACAAGGTTCAGCt < 2:214875/251‑1 (MQ=255) aCGTTACGGGAAGGAGTAGGTATAGAATGTTTGGATATCGCAGTAACGTGACAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGCTCGTGATGCCTGGCGTCTTGCGGCTTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAAAAAGGTTCAGCtt > 1:393825/1‑251 (MQ=255) cTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAg > 2:51909/1‑214 (MQ=255) cTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAg < 1:51909/214‑1 (MQ=255) ttAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAACCAAGGTTCAGCTTTCTACTTTGGCTTATTCTACCCGGACGAAAAAGCGATTATTGGCGTTGCCAATTTTTCCAAt > 2:381948/1‑251 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAAtgt < 2:87085/251‑1 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAAtgt < 1:335221/251‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGc < 2:48597/251‑1 (MQ=255) tGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTAtc > 1:173877/1‑251 (MQ=255) gCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGAtt > 1:294186/1‑251 (MQ=255) gCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGc > 1:294148/1‑250 (MQ=255) tCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCaaa > 2:284931/1‑250 (MQ=255) attattACGCAGAGAATCGCCATTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAgg > 2:220035/1‑251 (MQ=255) agaATCGCCATTTCCTCAAGCCC‑GGGAGCCAGCGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGGTTGGGCAAAAATGGCAGGGCAAAGGACTc > 1:19338/1‑250 (MQ=255) aTTTCCTCAAGCCC‑GGGAGCCAGTGCGCGACGAACGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCACTTTTTCCCATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGa > 2:262618/1‑249 (MQ=255) ccTCAAGCCC‑GGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCt < 2:217691/251‑1 (MQ=255) | AAATGGCATTTTGGACTTTTCACAGGGTCTGGTTGCGCAGGTATAGTAATACTTACAGCGTATTAAAGACGTTACGGGAAGGAGTAGGTATAGAATGTTTGGCTATCGCAGTAACGTGCCAAAAGTGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCT > NC_000913/1125468‑1125935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |