Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	181.9 / NA	60	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (32/28);  total (32/28)

GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGC  >  NC_000913/4636684‑4637166                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTc                                                                                                                                                                                                                                           <  1:450374/250‑1 (MQ=255)    gggCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCgcgc                                                                                                                                                                                                                                       >  1:30671/1‑251 (MQ=255)         tGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTAATGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAACCCCCGTTTCGCGCCAat                                                                                                                                                                                                                                   >  1:164954/1‑250 (MQ=255)                     cGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTa                                                                                                                                                                                                                      <  1:152667/251‑1 (MQ=255)                              gttgttGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTgc                                                                                                                                                                                                             <  1:329872/251‑1 (MQ=255)                                    gCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATg                                                                                                                                                                                                       <  1:282251/251‑1 (MQ=255)                                      tGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAAGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTTACAAAGTGCGCAATGa                                                                                                                                                                                                      >  1:373381/1‑250 (MQ=255)                                         gttATTTTTTACTGGTGGCGGTGGCAGCCTTGTTCGTACTGGCCATTTTTGTCAAAGAAGTGAAGCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTGGCTGGCGGAGCTGGCGCGTCCCGTGTTGCTCACTGTGGACCCAACGCATGGTCAAGTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGTCATTAACAAAGGGCGCAATGAatat                                                                                                                                                                                                  <  1:444551/249‑1 (MQ=255)                                           tATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACCACCTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGCATATc                                                                                                                                                                                                 >  2:88397/1‑250 (MQ=255)                                              tttttACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAAAGCACCGGAGGAGACGTTGATCGACACCGCAACGATGCTGGCGGAGCTGGGGCGTCCCGATTTGCTCTCTGGGGACCCAAAGCCTGGGCAACTGGCGCAAGCGTGTAATCAACTACACAATACCCCGTTTCGCGCCAATGACGGGGGCAGTAACAAAGTTCGCAATGAATATCAtat                                                                                                                                                                                             >  2:235417/1‑249 (MQ=255)                                                  tACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCtat                                                                                                                                                                                         >  1:227325/1‑251 (MQ=255)                                                   aCTGGGGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGGCAAAGAAGGTAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGGTGAGCGACACCGCAATGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCAGGGGCAACTGGCGTAGGGGTGTAATCAGCTACAACAGCCCCCGTTTCGCGGCAATATGGGTAGCATTAACAATGTGCGGAATGAAGATCATGTCtata                                                                                                                                                                                        <  2:214756/251‑1 (MQ=255)                                                           cggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGGTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTTCGCAATGAATATCATGTCTATATGACCGAt                                                                                                                                                                                <  1:181855/251‑1 (MQ=255)                                                           cggtggcAGCCTGGTTCGTACTGGACATTTTTGTCCAAGCAGTTAAACCGGGCGTGCGCAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGAACCAACGCATGGGCAACTGGCGCAGGCGTTTCATCAGCTACAACATCCACCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAt                                                                                                                                                                                >  2:47411/1‑251 (MQ=255)                                                            ggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCCCGCCACTATCGGTGGCATTAACCAAGCGCGCAATGAATATCATGTCTATATGACCGATg                                                                                                                                                                               >  2:123269/1‑251 (MQ=255)                                                                     cTGGTTCGTACTGGCCATTTTTGACCAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCCACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGCGGCATTAACAAAGTGCGCAATGAATCTCATGTCTATATGACCgcatcgcacgggc                                                                                                                                                                      >  2:90263/1‑239 (MQ=255)                                                                     cTGGTTAGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGAACAAACGCATGGGCCACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCCCGCCAATATCGGTGGCCATAACAAATTGCGCAATGAATATCATGTCTATATGACCGATgccccgggcc                                                                                                                                                                      >  2:369718/1‑243 (MQ=255)                                                                            gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGCGCGAAGAGCAACGGAGGGCACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCACTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAACAGCTACAACATCCACCGGTTCACGCCAATATCGGTGGCATTAACAAAGAGCGCCATGAAAATCATGTCTATTTGACAGATGCGCCGGGCAAAGTAtt                                                                                                                                                               >  2:154259/1‑251 (MQ=255)                                                                                 ggCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCg                                                                                                                                                          <  1:11469/251‑1 (MQ=255)                                                                                   ccATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAt                                                                                                                                                        <  1:371060/251‑1 (MQ=255)                                                                                     agttttGGCAAAGAAGGTAAACCGGGAGTGCGAAGAGCAACGGAGGGGACGTTGAGCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTc                                                                                                                                                      <  2:17312/249‑1 (MQ=255)                                                                                       ttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCGTGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCg                                                                                                                                                     <  1:331093/250‑1 (MQ=255)                                                                                        tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGc                                                                                                                                                   >  1:245227/1‑251 (MQ=255)                                                                                           gTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCaa                                                                                                                                                 >  1:337347/1‑250 (MQ=255)                                                                                                 gaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTa                                                                                                                                                                 >  1:233299/1‑228 (MQ=255)                                                                                                 gaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTa                                                                                                                                                                 <  2:233299/228‑1 (MQ=255)                                                                                                       aaaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTg                                                                                                                                     <  1:351350/250‑1 (MQ=255)                                                                                                          ccGGGCGTGCGACGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGAc                                                                                                                                  >  2:221352/1‑250 (MQ=255)                                                                                                          ccGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACa                                                                                                                                 <  1:111325/251‑1 (MQ=255)                                                                                                          ccGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAAGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGaa                                                                                                                                  >  1:356268/1‑249 (MQ=255)                                                                                                                gTGCGATGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGatt                                                                                                                            >  1:125772/1‑250 (MQ=255)                                                                                                                  gCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCCTGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGattatt                                                                                                                         >  2:345076/1‑251 (MQ=255)                                                                                                                           aaCGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTAGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTgg                                                                                                                 >  1:313875/1‑250 (MQ=255)                                                                                                                                aGGGGACGTTGATCGACACCGCAACGTTGCTGGAGGAGCTGGCGCGTCCAGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACCTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGAc                                                                                                           >  1:56697/1‑251 (MQ=255)                                                                                                                                         tGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAGAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCt                                                                                                   <  2:29484/250‑1 (MQ=255)                                                                                                                                          gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTa                                                                                                  >  1:345058/1‑250 (MQ=255)                                                                                                                                           aTCGACACCGCAACGTTGCTGGAGGCCCTGCAGCGCCCCGATTTGCTCTCTGGGACCCCCACGCATCGGCAACTGACGCCGGCGTTTAATCAGCTACCACATCCCCCGTTTCGCGCCAATATCGGTGGACTTAACAACGCGCGCAATGAAACTCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGCTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAAc                                                                                                >  2:61997/1‑251 (MQ=255)                                                                                                                                                      aaCGTTCCTGGCGTGGCTGGCGCGTCCCGGTTTGTTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGCGCGCAATGAATATCATGTCTATATGCCCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAAAAAGCCGATGGGCAGGATTATTCGCGCTGGAATGCCGTCTGGCTAAAGTTGCGTGGTc                                                                                     >  2:142211/1‑251 (MQ=255)                                                                                                                                                         gTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGt                                                                                  >  1:326936/1‑251 (MQ=255)                                                                                                                                                         gTTGCTGGCGGAGCTGGCGCGTCCCGATTTGATTTATGGGGACCCCACGCATGGGCAACCGGCGCAGGAGCTTAATCAGCTACAACATCCCCCGTTTCGCGCAAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATACGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGt                                                                                  >  1:190315/1‑251 (MQ=255)                                                                                                                                                            gCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATGATTCGCGCTGGAATTACGTCTGGCTAACGTGGCGTGGTCAGTATg                                                                               <  1:360854/251‑1 (MQ=255)                                                                                                                                                                gCGGGGCTGGCGCGGCCCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCTTTGGACAGGATTATTCGCGGTGGAATGACGGCTTGCTATCGCCGCGTGGTCAGGATGGTgc                                                                           <  2:52111/251‑1 (MQ=255)                                                                                                                                                                       tGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGCGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCgcagc                                                                    >  1:64121/1‑251 (MQ=255)                                                                                                                                                                        ggCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCgcagca                                                                   <  2:337347/251‑1 (MQ=255)                                                                                                                                                                          cgcgTCCCGATTTGCTCTCTGGGGACCCACCGCATGGGCAACTGTCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATAGCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTACCGTTGCGTGGTCAGTATGGTGCGCGCAGCACg                                                                 <  2:343871/251‑1 (MQ=255)                                                                                                                                                                             gTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGGACGTTg                                                              <  1:345076/251‑1 (MQ=255)                                                                                                                                                                                 cGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCaa                                                           >  2:4925/1‑250 (MQ=255)                                                                                                                                                                                       gCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTg                                                    <  2:140376/251‑1 (MQ=255)                                                                                                                                                                                          ctctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAGTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGGCTGGCTAACGTTTCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGc                                                   <  1:295575/249‑1 (MQ=255)                                                                                                                                                                                          ctctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTATAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGAGGCATTAACAAAGTGCGCAATGCCTATCATGTCTATATGCCCGATGCGCAGGGCAAAGCATTGTTCGATTCGGCAAATAAAGCCGTTGGCCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTCTGGTGCGCGCAGCACGTTGCAAAATCCTGCCg                                                 >  1:210092/1‑251 (MQ=255)                                                                                                                                                                                            ctGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGcc                                                  <  1:315905/248‑1 (MQ=255)                                                                                                                                                                                              ggggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCATCGCCAACATCCCCCGTTTCGCGCCAATAGCGGTGGCAGTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAATTTATTGTGCGATGCGGTAAATAAAGCCTTGGGACAGGATTATGCGCTCTGGAATGCGGTGTGGCTAACTTCGCTTGGTCGGTAAGGGGCGCgcattacgaggcaaaggctgtccgatc                                              <  2:284737/250‑25 (MQ=255)                                                                                                                                                                                               ggggcccAACCCATGGGCATCGGGCGCAGGGTTTTAATCAGCTACAACACCCCCCGTTCCGCGCCAATATCGGTGGCATGAACAAAGTGCGTAAGGAAGATCATGTCTATATGACCGATGCGCAGGGCAAAGCAGGGTTCGATTCGGCAAATAAGGCCGTTGGACAGTATTATTCGCGGTGGAGTGACGTGTGGCTAACGTTTCGTGGTGATTATGGTGCGTGCAGCACTGTGCATAATCCTGCCGATccc                                            <  2:386059/247‑1 (MQ=255)                                                                                                                                                                                               gggACCCAACGCAGGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATccc                                            <  2:338280/251‑1 (MQ=255)                                                                                                                                                                                                      aaCGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGtt                                     >  2:155001/1‑251 (MQ=255)                                                                                                                                                                                                        cGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCt                                   >  2:204032/1‑251 (MQ=255)                                                                                                                                                                                                                           ggCGTTAAATCAGCTACAACATCCCCCGTTTCGCCCCAATATCGGTGGCATTAACAACGTGCGCAATGAATATCATGTCTATATGACCGATGAGCAGGGCAAAGTATTGTACGATTCGGCACATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCAc                >  1:77501/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAAGCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGAc     <  2:227325/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCTACAACATCCCCCGTTTCGCGCCAAGATCGGTGGCATCAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCTGCATGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg    <  2:124746/251‑1 (MQ=255)                                                                                                                                                                                                                                         tACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGc  >  2:38921/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGC  >  NC_000913/4636684‑4637166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑