Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,291,496	C→T	P14S (CCC→TCC)	galU →	UTP‑‑glucose‑1‑phosphate uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,291,496	0	C	T	100.0%	52.3 / NA	17	P14S (CCC→TCC)	galU	UTP‑‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

CGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCCCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGC  >  NC_000913/1291269‑1291739                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cgGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAAATTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGACCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAgg                                                                                                                                                                                                                              >  2:264134/1‑251 (MQ=255)          tttGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCg                                                                                                                                                                                                                     >  1:131810/1‑251 (MQ=255)                                                    cATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGAt                                                                                                                                                                           >  1:127428/1‑251 (MQ=255)                                                                     ggcTTAAGTATGCTAACTCGCCTCCGTTTCAGAACTTAGCCCCTTCGGGGTGCAGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCGGCCATTAATACGAAAGTCAAAAAAGCCGTTAGCTCCGTTGCGGGATTAGGAACCAGGATGTTTCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCTCTTGGCGATAAGCCATTAATTCAATa                                                                                                                                                          <  2:142445/249‑1 (MQ=255)                                                                               tGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCATCGGGGTGCTGATATCCTGGGCCGCGATACAGAACTATGAACACGTTCAAAACCCGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGtgaat                                                                                                                                                >  1:356195/1‑251 (MQ=255)                                                                                                                         cTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTac                                                                                                       >  2:8194/1‑250 (MQ=255)                                                                                                                              ataCTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACt                                                                                                  <  1:8194/250‑1 (MQ=255)                                                                                                                                                          cacGTTCAAAACACGAACAGTCCAGGAGAATTTAAATTGCTGCCATTAATCCGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCAGTTCTGAAATTGTGCTGGTTACACACTCATCTGAAAACTCTATTGAAATCCACttt                                                                     <  2:177095/251‑1 (MQ=255)                                                                                                                                                           acGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTg                                                                    <  2:127428/251‑1 (MQ=255)                                                                                                                                                             gTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGTAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACGCATCGAAAAACTTTATTGAAAACCACTTTGAt                                                                  <  2:353355/251‑1 (MQ=255)                                                                                                                                                                  aaaCACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGCTTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATAcc                                                               >  2:150856/1‑249 (MQ=255)                                                                                                                                                                  aaaCACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAg                                                             <  1:112390/251‑1 (MQ=255)                                                                                                                                                                        gAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCAGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTg                                                        >  1:225682/1‑250 (MQ=255)                                                                                                                                                                                                   gCCATTAATACGAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtgt                            >  1:279160/1‑251 (MQ=255)                                                                                                                                                                                                    ccATTAATACGAAAGGCAAAAAAGACGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTa                           <  2:148637/251‑1 (MQ=255)                                                                                                                                                                                                              gAAAGTCAAAAAAGCCGTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAAc                 >  1:217449/1‑251 (MQ=255)                                                                                                                                                                                                                              gTTATCTCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCCCTTGTCGATAAGCCATTAATTCCATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGc  >  2:49536/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACACGAACAGTCCAGGAGAATTTAAATGGCTGCCATTAATACGAAAGTCAAAAAAGCCGTTATCCCCGTTGCGGGATTAGGAACCAGGATGTTGCCGGCGACGAAAGCCATCCCGAAAGAGATGCTGCCACTTGTCGATAAGCCATTAATTCAATACGTCGTGAATGAATGTATTGCGGCTGGCATTACTGAAATTGTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGC  >  NC_000913/1291269‑1291739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑