Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,519	G→A	A331V (GCG→GTG)	lacI ←	DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,519	0	G	A	100.0%	39.5 / NA	14	A331V (GCG→GTG)	lacI	DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/10);  total (4/10)

GAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCC  >  NC_000913/366396‑366764                                                                                                                            |                                                                                                                                                                                                                                                        gcgcagatgcgggcctcatgcgcgcgttgcgCTCCCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCGGCATTAATGAATCGTCCAACGCGCGGGGAGAGGCGGTTGGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCTCCGCCTGGCCCTGAGAGAGGTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGAt                                                                                                                         <  2:126428/228‑1 (MQ=255)       atgcggtccgcaatcgcgcgttgcgCTCCCGGCCCGCTTTCCAGGCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGTTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATggtggt                                                                                                                   <  2:165179/234‑1 (MQ=255)        tgcggtcctcaatcgcgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTTGGGACCCGGGGGGGTTTTCTTTTTACCCGTGGGGCGGGCAACAGCCGGATGCCCTTCACCCCCCGGCCCCGCGGGAGGTGGAGCAAGCGGTCCACCCTGGTTTGGCCCCCGC‑GGGGACAATCCTGTTTGGTGGTGGtt                                                                                                                  >  2:42138/17‑251 (MQ=255)                tcaatcgcgcgttgcgCTCACGGCCCGCTTTCCAGTCGGGAAACCTGTCTTGGCAGCGGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCCCCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgta                                                                                                                                                                           <  1:242090/179‑3 (MQ=255)                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa                                                                                                 <  2:75916/251‑1 (MQ=255)                              cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAAGCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGc                                                                                           <  2:144711/251‑1 (MQ=255)                                     ggCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTc                                                                                    <  1:4505/250‑1 (MQ=255)                                      gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTCGGTTTGGGGACCAGGGGGGGTTTTCTTTTTACCCGTGAGGCGGGGAACCGCCGATTGGCCCTCCCCGCCCGGCCCCGGGCGAGGTGCCGCAAGCCGGCCCCGCTGGGTTG‑GCCCCCCCGGCGAAAAACCTGTTTGTTGGGGGTTTACGGCggggtttaaccagaagggtctttc                                                                                    >  2:4505/1‑230 (MQ=255)                                               ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTCGGTAtcgtcg                                                                          <  2:307535/251‑1 (MQ=255)                                                       gaacccggTCGTGCCAGCTGCATTAATGAAGCGGCCATCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCCCCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTAAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTCGGTATCGTCGTATCCCAc                                                                  <  2:69226/244‑1 (MQ=255)                                                        aaaccgggcgggCCAGCGGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCGGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTCGGTATCGTCGTATCCCACt                                                                 <  2:116899/240‑1 (MQ=255)                                                                                  gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGTATTGGGGACCAGGGTGGGTTTTTTTTTTACCAGGGAGACGGGCAACAGCTGATTTGCCTTCACCGCCTGGCCCTGGGAGGGGTGCAGCAAGGGGGCCACGccgggtttcccccgc                                                                                                                                              >  1:289931/1‑136 (MQ=255)                                                                                  gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGTCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCcagc                                                                                                                                             <  2:289931/150‑1 (MQ=255)                                                                                                                       tGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATTGA‑CTGTCTTCGGGATCGGCGTTTCCCCCTACCGAGGTATCCCCCCCACCCCGCCGCCCGGCCTCCGTaactgcccgcctttcgccccgcgcc  >  2:154410/1‑228 (MQ=255)                                                                                                                            |                                                                                                                                                                                                                                                        GAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAT‑GAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCC  >  NC_000913/366396‑366764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑