Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,164,123	C→G	A162G (GCA→GGA)	btuB →	cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,164,123	0	C	G	100.0%	99.3 / NA	27	A162G (GCA→GGA)	btuB	cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/13);  total (14/13)

CAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCT  >  NC_000913/4163882‑4164365                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGCACGGAAATTTCAGGAGGGTgggg                                                                                                                                                                                                                                           >  1:107168/1‑251 (MQ=255)  aGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTgggg                                                                                                                                                                                                                                           >  2:288039/1‑250 (MQ=255)        cATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGGTTAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCaa                                                                                                                                                                                                                                     >  1:226596/1‑250 (MQ=255)             aTTTTTATTCGCGGTACAAATGCTAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGTCCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAg                                                                                                                                                                                                                                  <  2:129645/248‑1 (MQ=255)             aTTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGtt                                                                                                                                                                                                                                >  1:188295/1‑250 (MQ=255)             aTTTTTATTCGCGGAACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAAGAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                                                                                                                                                                                   <  2:107168/247‑1 (MQ=255)                       gcgttACAAATGCCAGGAATGTGTTGGTGTTAATTGAGGGCGTACGCCTGAAGTTGGCGGGGGTGAGGGGTGCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATAGATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCAGGGTGGTGAAGATCATCACGACGCGCGTTGAACCAAGAACGGAAATTTAAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                                                                                                                                                                                     <  2:24267/247‑1 (MQ=255)                            aCAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGCTGAACCCGGAACGGAAATTTCAGGCGGGCGGGGAAGCAATAGTTATCAGAACTATGATGt                                                                                                                                                                                                                >  1:269439/1‑251 (MQ=255)                              aaaTGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGGGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtct                                                                                                                                                                                                              <  2:135855/251‑1 (MQ=255)                                                             ggtgtttgtctgtaTCTGGCGTGCGTGAGGTGTTCTGCCGACCTTACCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACTCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAGCAACGGGGGGATAAGACACg                                                                                                                                                                               <  2:197124/238‑1 (MQ=255)                                                             ggCGTATGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACg                                                                                                                                                                               >  1:98408/1‑251 (MQ=255)                                                                     acTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTGGAATATTTCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGATCCCGGAACGGAAATTTCAGGACGGTGGTGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGTGTAAc                                                                                                                                                                         <  2:6319/248‑1 (MQ=255)                                                                        gAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg                                                                                                                                                                     <  1:288039/250‑1 (MQ=255)                                                                                   gggTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAt                                                                                                                                                         <  1:278287/251‑1 (MQ=255)                                                                                        aGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGcccc                                                                                                                                                    >  1:70704/1‑250 (MQ=255)                                                                                                     aCCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTat                                                                                                                                       >  2:79463/1‑251 (MQ=255)                                                                                                                      aTTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTAt                                                                                                                        <  2:284817/249‑1 (MQ=255)                                                                                                                                 ccAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGaa                                                                                                           <  1:353072/251‑1 (MQ=255)                                                                                                                                 ccAGCGTGTTGAATATATCCGGGTGCCGCGCTCCGCTGTTTATGGTGCCGATGCAAGAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACAGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGGTGCCGATGTGAAGACCGGaa                                                                                                           <  2:38106/251‑1 (MQ=255)                                                                                                                                  cAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAGAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTatgatg                                                                                                                                   <  1:184762/226‑1 (MQ=255)                                                                                                                                  cAGCGTGTTGAATATATCCGTGGGCCGAGCTCCGCTGTTTATGGCTCCGATGCAATAGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTCCGCAGCAACAACTGGGGGATAAGAAACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTatgatg                                                                                                                                   >  2:184762/1‑226 (MQ=255)                                                                                                                                                     gTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAAc                                                                                       <  2:188295/251‑1 (MQ=255)                                                                                                                                                                         tATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAAc                                                                   >  1:140068/1‑251 (MQ=255)                                                                                                                                                                                ccGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTAt                                                            >  1:118011/1‑251 (MQ=255)                                                                                                                                                                                                                      cgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCgg                      >  2:283889/1‑251 (MQ=255)                                                                                                                                                                                                                            gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTtgt                >  1:85913/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCggctatggct  >  1:278773/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCT  >  NC_000913/4163882‑4164365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑