Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,161,2480GT95.3% 147.5 / NA 44G61V (GGC→GTC) fabRDNA‑binding transcriptional repressor FabR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (28/13);  minor base A (2/0);  total (31/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTT  >  NC_000913/4161002‑4161483
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
cAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCCTTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCAtt                                                                                                                                                                                                                                         >  1:113188/1‑251 (MQ=255)
        tGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGAGGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCa                                                                                                                                                                                                                                  <  1:144864/250‑1 (MQ=255)
                                            acaatccgggAGCAATCCGGTTTGTTCATTTTCTGGTATAGTGCCAGCAGTACTTTTGGAAAGGATTCAGCCATCGTGAGGGGCGTAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCAGTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCATCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCTCCTCTTTTTATCGGCATTTCCGCGACGTATACGAACt                                                                                                                                                                                             <  1:308766/243‑1 (MQ=255)
                                                      aGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGAcc                                                                                                                                                                                   >  1:26739/1‑251 (MQ=255)
                                                              aGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGACGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtg                                                                                                                                                                            >  2:31184/1‑250 (MQ=255)
                                                                   ttccattcctgggTATAGTGCCAGCAGTTCTTTTGGCAAGGATTCAGACATGGTGAGGGGCGAAAGAGCACAAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATgag                                                                                                                                                                       <  2:61786/239‑1 (MQ=255)
                                                                    tctttttttgtatataGTGCAAGCAGTTCTTTTGGCAAGGATTAAGACAGCGTTGTGGGCTTAAGAGCCAAAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTGATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATgaga                                                                                                                                                                      <  1:31696/238‑1 (MQ=255)
                                                                                    gTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGc                                                                                                                                                     >  1:124574/1‑251 (MQ=255)
                                                                                      gCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGACATTGCTCCAACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGTTGAGAGCGGTTTAATGCTCCGccc                                                                                                                                                   >  2:188324/1‑250 (MQ=255)
                                                                                           cagTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGCAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTc                                                                                                                                               >  2:110608/1‑250 (MQ=255)
                                                                                                 ttttGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGcc                                                                                                                                        >  1:40550/1‑251 (MQ=255)
                                                                                                    tGGCAAGGATCCAGACAGCGGGTGGGGGGTAAAAGCGCAACAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAg                                                                                                                                      <  1:295886/250‑1 (MQ=255)
                                                                                                          ggATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTc                                                                                                                               >  2:228037/1‑251 (MQ=255)
                                                                                                                  aCATCGCGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATc                                                                                                                       >  1:94101/1‑251 (MQ=255)
                                                                                                                           tGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTATCCAATTAAGTGCTGAACGCCGATTAGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTAATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGACTGACCATGGTTGATGAGAGCGGTTTACTGCTACGCCACCTCATGCGCCCGGCGCGTCAGCGTATCGCCAAAGGc                                                                                                              >  1:288352/1‑251 (MQ=255)
                                                                                                                              gCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTAGATGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCggg                                                                                                           >  1:36527/1‑251 (MQ=255)
                                                                                                                                 tAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAgg                                                                                                        >  1:331415/1‑250 (MQ=255)
                                                                                                                                               aaaaaaaaacCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTc                                                                                          <  2:26739/247‑1 (MQ=255)
                                                                                                                                                  gAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTGGCTAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGt                                                                                       >  2:3461/1‑251 (MQ=255)
                                                                                                                                                      aaccccGCCGTTCGCTGGTGGCAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCCTTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGCCGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTCATGCTACGCCACCTCATGCGCCAGGCGCGTCCGCGTATCGCCAAAGGCGGGGGTGTGATcaccccccccgtctcc                                                                                   >  2:170686/4‑236 (MQ=255)
                                                                                                                                                      aaccccGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTcc                                                                                   <  1:188324/248‑1 (MQ=255)
                                                                                                                                                       aaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc                                                                                                                                                                                                  >  2:271315/1‑139 (MQ=255)
                                                                                                                                                       aaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc                                                                                                                                                                                                  <  1:271315/139‑1 (MQ=255)
                                                                                                                                                       aaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTccc                                                                                  >  1:299655/1‑250 (MQ=255)
                                                                                                                                                              cgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCCCCTCgggttcccaatttat                                                                           >  2:57127/1‑238 (MQ=255)
                                                                                                                                                                 tCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTCGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATgg                                                                         <  1:35916/250‑1 (MQ=255)
                                                                                                                                                                  cGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATgg                                                                         >  1:29528/1‑249 (MQ=255)
                                                                                                                                                                   gCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGCGTGTGATCCGCCCCTCGGTCTCCACCTTTATGGAGt                                                                      >  2:303168/1‑251 (MQ=255)
                                                                                                                                                                      ggtggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCa                                                                   <  1:228037/251‑1 (MQ=255)
                                                                                                                                                                               cGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGtaat                                                           >  2:217206/1‑250 (MQ=255)
                                                                                                                                                                                gCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGtaata                                                          >  1:206423/1‑250 (MQ=255)
                                                                                                                                                                                             tAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGTCTCCACCTTTATGGAGTTCATCGGTAATAATCCTAACGCCTTc                                            >  1:312093/1‑251 (MQ=255)
                                                                                                                                                                                                          cAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCCACTCTTTTTCTCGGCATTTCCGCGCCGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCACCTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCACCTCGGGCTCCACCTTTATGGCGTTCATCGGTCATAATCCTAACGACTTCCGGTTATTATTGc                               >  2:183271/1‑251 (MQ=255)
                                                                                                                                                                                                           aGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGGGATCCGCACCTCGGTCTCCAAATTTATGGAGTTCATCGGTAATAATACTAACGCCCTCCGGGTATTATTGCg                              >  2:79487/1‑251 (MQ=255)
                                                                                                                                                                                                           aGCTTCGCCAGCCAGTGTTTGCGTGAAGTGGCGCGTGAAGCGGACATTGCTCACACATCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGCATGAACATGATTGGTGAGAGCGGTTCAATGCAACGCAAACTCATGCGCCAGGAGCGTCCGCGTATCGCCAAAGGCGGGAGTGTGATCCGCGCACCGGGCTCCACCTTTATGGAGTTCATCGGACTTAATCCTAACGCCTTACGGTTATTATTGCg                              >  1:362822/1‑251 (MQ=255)
                                                                                                                                                                                                                   cAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCt                      >  1:132657/1‑251 (MQ=255)
                                                                                                                                                                                                                      ccTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCg                   <  2:299655/251‑1 (MQ=255)
                                                                                                                                                                                                                        tGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGATGATGCGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACCTTTATGGCGGTCATCGGGCATAATCCTAACGCCTTCCGGTTATTATTTCGGGAACGCTCCGGc                 >  2:150348/1‑251 (MQ=255)
                                                                                                                                                                                                                          ggTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCGCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCAc               <  2:286943/250‑1 (MQ=255)
                                                                                                                                                                                                                                 gTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCCCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGcc        >  1:171308/1‑250 (MQ=255)
                                                                                                                                                                                                                                 gTGAAGAGGCGCGTGAAGAGCGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGCACTGGGTCTGACCATGGCTGATGAGCGCGGTTTAATGCTACCCCCACTCCTGCGCCCGGCGCGTCAGCGTCTCGCCAAAGGCGGGAGTGTGCTCCGCCCCTCGGTCTCCCCCTTTATGGAGTTCCTCGGGAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGACACCTCCGCt        >  2:201023/1‑251 (MQ=255)
                                                                                                                                                                                                                                       tGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGttt  >  2:120252/1‑251 (MQ=255)
                                                                                                                                                                                                                                          cgcgTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATgg                                                                         >  1:473/1‑177 (MQ=255)
                                                                                                                                                                                                                                          cgcgTGAAGCGGTCATTGCTCCCACCTCTTTGTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATgg                                                                         <  2:473/177‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
CAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTT  >  NC_000913/4161002‑4161483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑